Phytochemical Properties and Inhibitory and Antioxidant Effects of the Decoction, Infusion and Hydro-Alcoholic Extract of Nepeta Race-mosa on α-Amylase and α-Glucosidase
Background: As an important plant belonging to the Nepeta genus, Nepeta racemosa has been reported to be widely used in traditional medicine. The present study was conducted to investigate the phytochemical content and antioxidant and antidiabetic potential of the decoction, infusion and hydro-alcoh...
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| Format: | Article |
| Language: | English |
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Bushehr University of Medical Sciences
2019-06-01
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| Series: | Iranian South Medical Journal |
| Subjects: | |
| Online Access: | http://ismj.bpums.ac.ir/browse.php?a_code=A-10-1-91&slc_lang=en&sid=1 |
| _version_ | 1831767374093615104 |
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| author | MohammadMehdi Zarrabi Behvar Asghari Ammar Maryamabadi GholamHosseain Mohebbi Saeid Rashvand |
| author_facet | MohammadMehdi Zarrabi Behvar Asghari Ammar Maryamabadi GholamHosseain Mohebbi Saeid Rashvand |
| author_sort | MohammadMehdi Zarrabi |
| collection | DOAJ |
| description | Background: As an important plant belonging to the Nepeta genus, Nepeta racemosa has been reported to be widely used in traditional medicine. The present study was conducted to investigate the phytochemical content and antioxidant and antidiabetic potential of the decoction, infusion and hydro-alcoholic extract of this plant.
Materials and Methods: The total phenolic content of the samples was determined using a slightly- modified Folin–Ciocalteu method. The DPPH free radical scavenging method was used to evaluate the antioxidant activities, and the Vanillin-Sulphuric Acid Method for the saponin content of the extract. Moreover, their inhibitory effects on α-amylase and α-glucosidase were investigated based on standard methods.
Results: The phenolic content of the plant decoction was found to be 222.8 mg of gallic acid equivalent per gram of dried extract and its flavonoid content 87.8 mg of quercetin equivalent per gram of dried extract. The highest saponin content obtained was associated with the hydro-alcoholic extract of the plant with 41.9 mg of quillaja equivalent per gram of dried extract. The plant decoction also showed the highest potential compared to the other samples in terms of antioxidant properties investigated using the DPPH and FRAP methods. Furthermore, the plant decoction showed great potential in terms of its inhibitory effects on α-amylase with 9.45 µmol of acarbose equivalent per gram of dried extract, and α-glucosidase with 33.57 µmol of acarbose equivalent per gram of dried extract.
Conclusion: According to the results, Nepeta racemosa was found to exhibit high levels of antioxidant and antidiabetic abilities, which are directly proportional to its phenolic and flavonoid contents. |
| first_indexed | 2024-12-22T06:41:11Z |
| format | Article |
| id | doaj.art-4e1ff58a878244269c815eb939b19648 |
| institution | Directory Open Access Journal |
| issn | 1735-4374 1735-6954 |
| language | English |
| last_indexed | 2024-12-22T06:41:11Z |
| publishDate | 2019-06-01 |
| publisher | Bushehr University of Medical Sciences |
| record_format | Article |
| series | Iranian South Medical Journal |
| spelling | doaj.art-4e1ff58a878244269c815eb939b196482022-12-21T18:35:26ZengBushehr University of Medical SciencesIranian South Medical Journal1735-43741735-69542019-06-0122290105Phytochemical Properties and Inhibitory and Antioxidant Effects of the Decoction, Infusion and Hydro-Alcoholic Extract of Nepeta Race-mosa on α-Amylase and α-GlucosidaseMohammadMehdi Zarrabi0Behvar Asghari1Ammar Maryamabadi2GholamHosseain Mohebbi3Saeid Rashvand4 Department of Plant Production and Breeding Engineering, school of Engineering and Technology, Imam Khomeini International University, Qazvin, Iran Department of Plant Production and Breeding Engineering, school of Engineering and Technology, Imam Khomeini International University, Qazvin, Iran Research and development Department, Shakheh Zeytoon Lian Inspection Co, Bushehr, Iran The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran Department of Forests and Rangeland, Qazvin Agricultural and Natural Resources Research and Education Center, AREEO, Qazvin, Iran Background: As an important plant belonging to the Nepeta genus, Nepeta racemosa has been reported to be widely used in traditional medicine. The present study was conducted to investigate the phytochemical content and antioxidant and antidiabetic potential of the decoction, infusion and hydro-alcoholic extract of this plant. Materials and Methods: The total phenolic content of the samples was determined using a slightly- modified Folin–Ciocalteu method. The DPPH free radical scavenging method was used to evaluate the antioxidant activities, and the Vanillin-Sulphuric Acid Method for the saponin content of the extract. Moreover, their inhibitory effects on α-amylase and α-glucosidase were investigated based on standard methods. Results: The phenolic content of the plant decoction was found to be 222.8 mg of gallic acid equivalent per gram of dried extract and its flavonoid content 87.8 mg of quercetin equivalent per gram of dried extract. The highest saponin content obtained was associated with the hydro-alcoholic extract of the plant with 41.9 mg of quillaja equivalent per gram of dried extract. The plant decoction also showed the highest potential compared to the other samples in terms of antioxidant properties investigated using the DPPH and FRAP methods. Furthermore, the plant decoction showed great potential in terms of its inhibitory effects on α-amylase with 9.45 µmol of acarbose equivalent per gram of dried extract, and α-glucosidase with 33.57 µmol of acarbose equivalent per gram of dried extract. Conclusion: According to the results, Nepeta racemosa was found to exhibit high levels of antioxidant and antidiabetic abilities, which are directly proportional to its phenolic and flavonoid contents.http://ismj.bpums.ac.ir/browse.php?a_code=A-10-1-91&slc_lang=en&sid=1Nepeta racemosaphytochemical propertiesantioxidant effectsα-Amylase Inhibitorα-Glucosidase Inhibitor |
| spellingShingle | MohammadMehdi Zarrabi Behvar Asghari Ammar Maryamabadi GholamHosseain Mohebbi Saeid Rashvand Phytochemical Properties and Inhibitory and Antioxidant Effects of the Decoction, Infusion and Hydro-Alcoholic Extract of Nepeta Race-mosa on α-Amylase and α-Glucosidase Iranian South Medical Journal Nepeta racemosa phytochemical properties antioxidant effects α-Amylase Inhibitor α-Glucosidase Inhibitor |
| title | Phytochemical Properties and Inhibitory and Antioxidant Effects of the Decoction, Infusion and Hydro-Alcoholic Extract of Nepeta Race-mosa on α-Amylase and α-Glucosidase |
| title_full | Phytochemical Properties and Inhibitory and Antioxidant Effects of the Decoction, Infusion and Hydro-Alcoholic Extract of Nepeta Race-mosa on α-Amylase and α-Glucosidase |
| title_fullStr | Phytochemical Properties and Inhibitory and Antioxidant Effects of the Decoction, Infusion and Hydro-Alcoholic Extract of Nepeta Race-mosa on α-Amylase and α-Glucosidase |
| title_full_unstemmed | Phytochemical Properties and Inhibitory and Antioxidant Effects of the Decoction, Infusion and Hydro-Alcoholic Extract of Nepeta Race-mosa on α-Amylase and α-Glucosidase |
| title_short | Phytochemical Properties and Inhibitory and Antioxidant Effects of the Decoction, Infusion and Hydro-Alcoholic Extract of Nepeta Race-mosa on α-Amylase and α-Glucosidase |
| title_sort | phytochemical properties and inhibitory and antioxidant effects of the decoction infusion and hydro alcoholic extract of nepeta race mosa on α amylase and α glucosidase |
| topic | Nepeta racemosa phytochemical properties antioxidant effects α-Amylase Inhibitor α-Glucosidase Inhibitor |
| url | http://ismj.bpums.ac.ir/browse.php?a_code=A-10-1-91&slc_lang=en&sid=1 |
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