Evaluation of the Coagulant Effect of Zanjani and Latifi Viper Snake Venom Endemic in Iran
The venom of the viper is very important in pharmaceutical usage, such as in the process of coagulation during medical care. This study aims to evaluate the coagulant factor of Latifi and Zanjani viper venom. In the current study, after electrophoresis of proteins found in viper venom, all of the th...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Shahid Beheshti University of Medical Sciences
2016-10-01
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| Series: | Trends in Peptide and Protein Sciences |
| Subjects: | |
| Online Access: | https://journals.sbmu.ac.ir/protein/article/view/13130 |
| _version_ | 1819290793228107776 |
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| author | Milad Taherian Hajar Yaghoobi Mojgan Bandehpour |
| author_facet | Milad Taherian Hajar Yaghoobi Mojgan Bandehpour |
| author_sort | Milad Taherian |
| collection | DOAJ |
| description | The venom of the viper is very important in pharmaceutical usage, such as in the process of coagulation during medical care. This study aims to evaluate the coagulant factor of Latifi and Zanjani viper venom. In the current study, after electrophoresis of proteins found in viper venom, all of the thick and strong bands of proteins were isolated and prepared for examination of coagulation characteristics, like pro-thrombin time (PTT) and active partial thromboplastic time (APTT), followed by further study by mass spectroscopy. In this way, 11 bands of proteins were recognized in sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). After PT and APTT tests, one common band in 26 KDa could lead to coagulation of blood plasma in less than one second. Mass spectroscopy identified this band as serine protease isoform4. The results confirmed the coagulation effect of a 26 KDa protein fraction of venom from Latifi and Zanjani vipers.
Highlights
• The viper snake venom is mostly blood-oriented and leads to blood coagulation.
• The snakes venom, including vipers venom endemic of Iran could be applied for pharmaceutical purposes.
• A 26KD protein was the most effective component of viper venom showed plasma coagulation.
• The results showed this protein was related to serine protease enzyme of snake venom. |
| first_indexed | 2024-12-24T03:28:23Z |
| format | Article |
| id | doaj.art-4e2583bf46e446bc9f1e007b8c6eca3e |
| institution | Directory Open Access Journal |
| issn | 2538-2446 |
| language | English |
| last_indexed | 2024-12-24T03:28:23Z |
| publishDate | 2016-10-01 |
| publisher | Shahid Beheshti University of Medical Sciences |
| record_format | Article |
| series | Trends in Peptide and Protein Sciences |
| spelling | doaj.art-4e2583bf46e446bc9f1e007b8c6eca3e2022-12-21T17:17:16ZengShahid Beheshti University of Medical SciencesTrends in Peptide and Protein Sciences2538-24462016-10-0111273010.22037/tpps.v1i1.1313013130Evaluation of the Coagulant Effect of Zanjani and Latifi Viper Snake Venom Endemic in IranMilad TaherianHajar YaghoobiMojgan BandehpourThe venom of the viper is very important in pharmaceutical usage, such as in the process of coagulation during medical care. This study aims to evaluate the coagulant factor of Latifi and Zanjani viper venom. In the current study, after electrophoresis of proteins found in viper venom, all of the thick and strong bands of proteins were isolated and prepared for examination of coagulation characteristics, like pro-thrombin time (PTT) and active partial thromboplastic time (APTT), followed by further study by mass spectroscopy. In this way, 11 bands of proteins were recognized in sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). After PT and APTT tests, one common band in 26 KDa could lead to coagulation of blood plasma in less than one second. Mass spectroscopy identified this band as serine protease isoform4. The results confirmed the coagulation effect of a 26 KDa protein fraction of venom from Latifi and Zanjani vipers. Highlights • The viper snake venom is mostly blood-oriented and leads to blood coagulation. • The snakes venom, including vipers venom endemic of Iran could be applied for pharmaceutical purposes. • A 26KD protein was the most effective component of viper venom showed plasma coagulation. • The results showed this protein was related to serine protease enzyme of snake venom.https://journals.sbmu.ac.ir/protein/article/view/13130latifi viperzanjani vipersds pagepttaptt |
| spellingShingle | Milad Taherian Hajar Yaghoobi Mojgan Bandehpour Evaluation of the Coagulant Effect of Zanjani and Latifi Viper Snake Venom Endemic in Iran Trends in Peptide and Protein Sciences latifi viper zanjani viper sds page ptt aptt |
| title | Evaluation of the Coagulant Effect of Zanjani and Latifi Viper Snake Venom Endemic in Iran |
| title_full | Evaluation of the Coagulant Effect of Zanjani and Latifi Viper Snake Venom Endemic in Iran |
| title_fullStr | Evaluation of the Coagulant Effect of Zanjani and Latifi Viper Snake Venom Endemic in Iran |
| title_full_unstemmed | Evaluation of the Coagulant Effect of Zanjani and Latifi Viper Snake Venom Endemic in Iran |
| title_short | Evaluation of the Coagulant Effect of Zanjani and Latifi Viper Snake Venom Endemic in Iran |
| title_sort | evaluation of the coagulant effect of zanjani and latifi viper snake venom endemic in iran |
| topic | latifi viper zanjani viper sds page ptt aptt |
| url | https://journals.sbmu.ac.ir/protein/article/view/13130 |
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