Preparation and Characterization of Extracellular Matrix Hydrogels Derived from Acellular Cartilage Tissue
Decellularized matrices can effectively reduce severe immune rejection with their cells and eliminated nucleic acid material and provide specific environments for tissue repair or tissue regeneration. In this study, we prepared acellular cartilage matrix (ACM) powder through the decellularization me...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/13/4/279 |
| _version_ | 1797456990656528384 |
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| author | Tsong-Hann Yu Tsu-Te Yeh Chen-Ying Su Ni-Yin Yu I-Cheng Chen Hsu-Wei Fang |
| author_facet | Tsong-Hann Yu Tsu-Te Yeh Chen-Ying Su Ni-Yin Yu I-Cheng Chen Hsu-Wei Fang |
| author_sort | Tsong-Hann Yu |
| collection | DOAJ |
| description | Decellularized matrices can effectively reduce severe immune rejection with their cells and eliminated nucleic acid material and provide specific environments for tissue repair or tissue regeneration. In this study, we prepared acellular cartilage matrix (ACM) powder through the decellularization method and developed ACM hydrogels by physical, chemical, and enzymatic digestion methods. The results demonstrated that the small size group of ACM hydrogels exhibited better gel conditions when the concentration of ACM hydrogels was 30 and 20 mg/mL in 1N HCl through parameter adjustment. The data also confirmed that the ACM hydrogels retained the main components of cartilage: 61.18% of glycosaminoglycan (GAG) and 78.29% of collagen, with 99.61% of its DNA removed compared to samples without the decellularization procedure (set as 100%). Through turbidimetric gelation kinetics, hydrogel rheological property analysis, and hydrogel tissue physical property testing, this study also revealed that increasing hydrogel concentration is helpful for gelation. Besides, the ex vivo test confirmed that a higher concentration of ACM hydrogels had good adhesive properties and could fill in cartilage defects adequately. This study offers useful information for developing and manufacturing ACM hydrogels to serve as potential alternative scaffolds for future cartilage defect treatment. |
| first_indexed | 2024-03-09T16:15:43Z |
| format | Article |
| id | doaj.art-4e2e2c6da24a48d595e921a71e5c3ad5 |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-03-09T16:15:43Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-4e2e2c6da24a48d595e921a71e5c3ad52023-11-24T15:50:43ZengMDPI AGJournal of Functional Biomaterials2079-49832022-12-0113427910.3390/jfb13040279Preparation and Characterization of Extracellular Matrix Hydrogels Derived from Acellular Cartilage TissueTsong-Hann Yu0Tsu-Te Yeh1Chen-Ying Su2Ni-Yin Yu3I-Cheng Chen4Hsu-Wei Fang5Department of Orthopedics, Tri-Service General Hospital, National Defense Medical Center, No. 325, Sec. 2, Chenggong Rd., Taipei 114202, TaiwanDepartment of Orthopedics, Tri-Service General Hospital, National Defense Medical Center, No. 325, Sec. 2, Chenggong Rd., Taipei 114202, TaiwanDepartment of Chemical Engineering and Biotechnology, National Taipei University of Technology, No. 1, Sec. 3, Zhongxiao E. Rd., Taipei 10608, TaiwanDepartment of Chemical Engineering and Biotechnology, National Taipei University of Technology, No. 1, Sec. 3, Zhongxiao E. Rd., Taipei 10608, TaiwanDepartment of Chemical Engineering and Biotechnology, National Taipei University of Technology, No. 1, Sec. 3, Zhongxiao E. Rd., Taipei 10608, TaiwanDepartment of Chemical Engineering and Biotechnology, National Taipei University of Technology, No. 1, Sec. 3, Zhongxiao E. Rd., Taipei 10608, TaiwanDecellularized matrices can effectively reduce severe immune rejection with their cells and eliminated nucleic acid material and provide specific environments for tissue repair or tissue regeneration. In this study, we prepared acellular cartilage matrix (ACM) powder through the decellularization method and developed ACM hydrogels by physical, chemical, and enzymatic digestion methods. The results demonstrated that the small size group of ACM hydrogels exhibited better gel conditions when the concentration of ACM hydrogels was 30 and 20 mg/mL in 1N HCl through parameter adjustment. The data also confirmed that the ACM hydrogels retained the main components of cartilage: 61.18% of glycosaminoglycan (GAG) and 78.29% of collagen, with 99.61% of its DNA removed compared to samples without the decellularization procedure (set as 100%). Through turbidimetric gelation kinetics, hydrogel rheological property analysis, and hydrogel tissue physical property testing, this study also revealed that increasing hydrogel concentration is helpful for gelation. Besides, the ex vivo test confirmed that a higher concentration of ACM hydrogels had good adhesive properties and could fill in cartilage defects adequately. This study offers useful information for developing and manufacturing ACM hydrogels to serve as potential alternative scaffolds for future cartilage defect treatment.https://www.mdpi.com/2079-4983/13/4/279acellular extracellular matrixhydrogeldecellularizationscaffoldtissue adhesiontissue engineering |
| spellingShingle | Tsong-Hann Yu Tsu-Te Yeh Chen-Ying Su Ni-Yin Yu I-Cheng Chen Hsu-Wei Fang Preparation and Characterization of Extracellular Matrix Hydrogels Derived from Acellular Cartilage Tissue Journal of Functional Biomaterials acellular extracellular matrix hydrogel decellularization scaffold tissue adhesion tissue engineering |
| title | Preparation and Characterization of Extracellular Matrix Hydrogels Derived from Acellular Cartilage Tissue |
| title_full | Preparation and Characterization of Extracellular Matrix Hydrogels Derived from Acellular Cartilage Tissue |
| title_fullStr | Preparation and Characterization of Extracellular Matrix Hydrogels Derived from Acellular Cartilage Tissue |
| title_full_unstemmed | Preparation and Characterization of Extracellular Matrix Hydrogels Derived from Acellular Cartilage Tissue |
| title_short | Preparation and Characterization of Extracellular Matrix Hydrogels Derived from Acellular Cartilage Tissue |
| title_sort | preparation and characterization of extracellular matrix hydrogels derived from acellular cartilage tissue |
| topic | acellular extracellular matrix hydrogel decellularization scaffold tissue adhesion tissue engineering |
| url | https://www.mdpi.com/2079-4983/13/4/279 |
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