Mesencephalic basolateral domain specification is dependent on Sonic Hedgehog
In the study of central nervous system morphogenesis, the identification of new molecular markers allows us to identify domains along the antero-posterior and dorso-ventral axes. In the past years, the alar and basal plates of the midbrain have been divided into different domains. The precise locati...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2015-02-01
|
| Series: | Frontiers in Neuroanatomy |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fnana.2015.00012/full |
| _version_ | 1811303620039147520 |
|---|---|
| author | Jesus E. Martinez-Lopez Juan A Moreno-Bravo M. Pilar eMadrigal Salvador eMartinez Salvador eMartinez Eduardo ePuelles |
| author_facet | Jesus E. Martinez-Lopez Juan A Moreno-Bravo M. Pilar eMadrigal Salvador eMartinez Salvador eMartinez Eduardo ePuelles |
| author_sort | Jesus E. Martinez-Lopez |
| collection | DOAJ |
| description | In the study of central nervous system morphogenesis, the identification of new molecular markers allows us to identify domains along the antero-posterior and dorso-ventral axes. In the past years, the alar and basal plates of the midbrain have been divided into different domains. The precise location of the alar-basal boundary is still under discussion. We have identified Barhl1, Nhlh1 and Six3 as appropriate molecular markers to the adjacent domains of this transition. The description of their expression patterns and the contribution to the different mesencephalic populations corroborated their role in the specification of these domains. We studied the influence of Sonic Hedgehog on these markers and therefore on the specification of these territories. The lack of this morphogen produced severe alterations in the expression pattern of Barhl1 and Nhlh1 with consequent misspecification of the basolateral domain. Six3 expression was apparently unaffected, however its distribution changed leading to altered basal domains. In this study we confirmed the localization of the alar-basal boundary dorsal to the basolateral domain and demonstrated that the development of the basolateral domain highly depends on Shh. |
| first_indexed | 2024-04-13T07:51:40Z |
| format | Article |
| id | doaj.art-4e319496a4be4ad9a1037b6f0587a9c7 |
| institution | Directory Open Access Journal |
| issn | 1662-5129 |
| language | English |
| last_indexed | 2024-04-13T07:51:40Z |
| publishDate | 2015-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroanatomy |
| spelling | doaj.art-4e319496a4be4ad9a1037b6f0587a9c72022-12-22T02:55:31ZengFrontiers Media S.A.Frontiers in Neuroanatomy1662-51292015-02-01910.3389/fnana.2015.00012127523Mesencephalic basolateral domain specification is dependent on Sonic HedgehogJesus E. Martinez-Lopez0Juan A Moreno-Bravo1M. Pilar eMadrigal2Salvador eMartinez3Salvador eMartinez4Eduardo ePuelles5Universidad Miguel Hernández de ElcheUniversidad Miguel Hernández de ElcheUniversidad Miguel Hernández de ElcheUniversidad Miguel Hernández de ElcheInstituto Murciano de Investigación Biomédica (CIBERSAM)Universidad Miguel Hernández de ElcheIn the study of central nervous system morphogenesis, the identification of new molecular markers allows us to identify domains along the antero-posterior and dorso-ventral axes. In the past years, the alar and basal plates of the midbrain have been divided into different domains. The precise location of the alar-basal boundary is still under discussion. We have identified Barhl1, Nhlh1 and Six3 as appropriate molecular markers to the adjacent domains of this transition. The description of their expression patterns and the contribution to the different mesencephalic populations corroborated their role in the specification of these domains. We studied the influence of Sonic Hedgehog on these markers and therefore on the specification of these territories. The lack of this morphogen produced severe alterations in the expression pattern of Barhl1 and Nhlh1 with consequent misspecification of the basolateral domain. Six3 expression was apparently unaffected, however its distribution changed leading to altered basal domains. In this study we confirmed the localization of the alar-basal boundary dorsal to the basolateral domain and demonstrated that the development of the basolateral domain highly depends on Shh.http://journal.frontiersin.org/Journal/10.3389/fnana.2015.00012/fullShhmidbrainSix3Barhl1Nhlh1basolateral domain |
| spellingShingle | Jesus E. Martinez-Lopez Juan A Moreno-Bravo M. Pilar eMadrigal Salvador eMartinez Salvador eMartinez Eduardo ePuelles Mesencephalic basolateral domain specification is dependent on Sonic Hedgehog Frontiers in Neuroanatomy Shh midbrain Six3 Barhl1 Nhlh1 basolateral domain |
| title | Mesencephalic basolateral domain specification is dependent on Sonic Hedgehog |
| title_full | Mesencephalic basolateral domain specification is dependent on Sonic Hedgehog |
| title_fullStr | Mesencephalic basolateral domain specification is dependent on Sonic Hedgehog |
| title_full_unstemmed | Mesencephalic basolateral domain specification is dependent on Sonic Hedgehog |
| title_short | Mesencephalic basolateral domain specification is dependent on Sonic Hedgehog |
| title_sort | mesencephalic basolateral domain specification is dependent on sonic hedgehog |
| topic | Shh midbrain Six3 Barhl1 Nhlh1 basolateral domain |
| url | http://journal.frontiersin.org/Journal/10.3389/fnana.2015.00012/full |
| work_keys_str_mv | AT jesusemartinezlopez mesencephalicbasolateraldomainspecificationisdependentonsonichedgehog AT juanamorenobravo mesencephalicbasolateraldomainspecificationisdependentonsonichedgehog AT mpilaremadrigal mesencephalicbasolateraldomainspecificationisdependentonsonichedgehog AT salvadoremartinez mesencephalicbasolateraldomainspecificationisdependentonsonichedgehog AT salvadoremartinez mesencephalicbasolateraldomainspecificationisdependentonsonichedgehog AT eduardoepuelles mesencephalicbasolateraldomainspecificationisdependentonsonichedgehog |