Members of the <i>Capsicum annuum</i> CaTrxh Family Respond to High Temperature and Exhibit Dynamic Hetero/Homo Interactions
Climate change adversely affects the water and temperature conditions required for plant growth, leading to a decrease in yield. In high temperatures, oxidative stress causes cellular damage in plant cells, which is a negative factor for crop production. Thioredoxin (Trx) is a small redox protein co...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/3/1729 |
| _version_ | 1797318656317718528 |
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| author | Suji Hong Sung Un Huh |
| author_facet | Suji Hong Sung Un Huh |
| author_sort | Suji Hong |
| collection | DOAJ |
| description | Climate change adversely affects the water and temperature conditions required for plant growth, leading to a decrease in yield. In high temperatures, oxidative stress causes cellular damage in plant cells, which is a negative factor for crop production. Thioredoxin (Trx) is a small redox protein containing a conserved WC(G/P)PC motif that catalyzes the exchange of disulfide bonds. It is known to play an important role in maintaining cellular redox homeostasis. Trx proteins are widely distributed across various subcellular locations, and they play a crucial role in responding to cellular stresses. In this study, seven <i>CaTrxh</i>-type genes present in pepper were identified and the <i>CaTrxh</i>-type family was classified into three subgroups. <i>CaTrxh</i> genes responded to heat stress. Moreover, subcellular locations of the CaTrxh family exhibited dynamic patterns in normal conditions, and we observed relocalizations in heat stress conditions. Each CaTrxh family protein member formed homo-/heteromeric protein complexes in BiFC assay. Unexpectedly, subgroup III CaTrxh9 and CaTrxh10 can recruit subgroup I and II CaTrxh proteins into the plasma membrane. Thus, the function of the CaTrxh-type family is expected to play a protective role in the cell in response to high-temperature stress via protein complex formations. CaTrxh may have potential applications in the development of crops with enhanced tolerance to oxidative stress. |
| first_indexed | 2024-03-08T03:55:30Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T03:55:30Z |
| publishDate | 2024-01-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-4e37dfc751704451a89f5f7ba2393cae2024-02-09T15:14:22ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-01-01253172910.3390/ijms25031729Members of the <i>Capsicum annuum</i> CaTrxh Family Respond to High Temperature and Exhibit Dynamic Hetero/Homo InteractionsSuji Hong0Sung Un Huh1Department of Biological Science, Kunsan National University, Gunsan 54150, Republic of KoreaDepartment of Biological Science, Kunsan National University, Gunsan 54150, Republic of KoreaClimate change adversely affects the water and temperature conditions required for plant growth, leading to a decrease in yield. In high temperatures, oxidative stress causes cellular damage in plant cells, which is a negative factor for crop production. Thioredoxin (Trx) is a small redox protein containing a conserved WC(G/P)PC motif that catalyzes the exchange of disulfide bonds. It is known to play an important role in maintaining cellular redox homeostasis. Trx proteins are widely distributed across various subcellular locations, and they play a crucial role in responding to cellular stresses. In this study, seven <i>CaTrxh</i>-type genes present in pepper were identified and the <i>CaTrxh</i>-type family was classified into three subgroups. <i>CaTrxh</i> genes responded to heat stress. Moreover, subcellular locations of the CaTrxh family exhibited dynamic patterns in normal conditions, and we observed relocalizations in heat stress conditions. Each CaTrxh family protein member formed homo-/heteromeric protein complexes in BiFC assay. Unexpectedly, subgroup III CaTrxh9 and CaTrxh10 can recruit subgroup I and II CaTrxh proteins into the plasma membrane. Thus, the function of the CaTrxh-type family is expected to play a protective role in the cell in response to high-temperature stress via protein complex formations. CaTrxh may have potential applications in the development of crops with enhanced tolerance to oxidative stress.https://www.mdpi.com/1422-0067/25/3/1729Thioredoxin (Trx)climate changeabiotic stress<i>Capsicum annuum</i> |
| spellingShingle | Suji Hong Sung Un Huh Members of the <i>Capsicum annuum</i> CaTrxh Family Respond to High Temperature and Exhibit Dynamic Hetero/Homo Interactions International Journal of Molecular Sciences Thioredoxin (Trx) climate change abiotic stress <i>Capsicum annuum</i> |
| title | Members of the <i>Capsicum annuum</i> CaTrxh Family Respond to High Temperature and Exhibit Dynamic Hetero/Homo Interactions |
| title_full | Members of the <i>Capsicum annuum</i> CaTrxh Family Respond to High Temperature and Exhibit Dynamic Hetero/Homo Interactions |
| title_fullStr | Members of the <i>Capsicum annuum</i> CaTrxh Family Respond to High Temperature and Exhibit Dynamic Hetero/Homo Interactions |
| title_full_unstemmed | Members of the <i>Capsicum annuum</i> CaTrxh Family Respond to High Temperature and Exhibit Dynamic Hetero/Homo Interactions |
| title_short | Members of the <i>Capsicum annuum</i> CaTrxh Family Respond to High Temperature and Exhibit Dynamic Hetero/Homo Interactions |
| title_sort | members of the i capsicum annuum i catrxh family respond to high temperature and exhibit dynamic hetero homo interactions |
| topic | Thioredoxin (Trx) climate change abiotic stress <i>Capsicum annuum</i> |
| url | https://www.mdpi.com/1422-0067/25/3/1729 |
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