Using non–invasive genetic techniques to assist in maned wolf conservation in a remnant fragment of the Brazilian Cerrado

The maned wolf is a South American canid considered a keystone species of the Cerrado. We performed a genetic assessment of maned wolves that inhabit a small remnant fragment of the Cerrado in Brazil. We collected 84 fecal samples over a year and also included two tissue samples from road–killed ani...

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Main Authors: Mannise, N., Trovati, R. G., Duarte, J. M. B., Maldonado, J. E., González, S.
Format: Article
Language:English
Published: Museu de Ciències Naturals de Barcelona 2018-08-01
Series:Animal Biodiversity and Conservation
Subjects:
Online Access:http://abc.museucienciesjournals.cat/files/ABC_41-2_pp_315-319-color.pdf
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author Mannise, N.
Trovati, R. G.
Duarte, J. M. B.
Maldonado, J. E.
González, S.
author_facet Mannise, N.
Trovati, R. G.
Duarte, J. M. B.
Maldonado, J. E.
González, S.
author_sort Mannise, N.
collection DOAJ
description The maned wolf is a South American canid considered a keystone species of the Cerrado. We performed a genetic assessment of maned wolves that inhabit a small remnant fragment of the Cerrado in Brazil. We collected 84 fecal samples over a year and also included two tissue samples from road–killed animals. We successfully identified the species, sex, and individuals using molecular markers. Using microsatellite loci analysis we identified 13 different individuals, eight females and five males. The genetic variability level found and the high number of individuals detected indicates the presence of an open population.
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spelling doaj.art-4e3886c775bc4dd8a180291459b796042022-12-21T22:37:41ZengMuseu de Ciències Naturals de BarcelonaAnimal Biodiversity and Conservation1578-665X2018-08-01412315319Using non–invasive genetic techniques to assist in maned wolf conservation in a remnant fragment of the Brazilian CerradoMannise, N.Trovati, R. G.Duarte, J. M. B.Maldonado, J. E. González, S.The maned wolf is a South American canid considered a keystone species of the Cerrado. We performed a genetic assessment of maned wolves that inhabit a small remnant fragment of the Cerrado in Brazil. We collected 84 fecal samples over a year and also included two tissue samples from road–killed animals. We successfully identified the species, sex, and individuals using molecular markers. Using microsatellite loci analysis we identified 13 different individuals, eight females and five males. The genetic variability level found and the high number of individuals detected indicates the presence of an open population.http://abc.museucienciesjournals.cat/files/ABC_41-2_pp_315-319-color.pdfFecal DNAMicrosatellite lociZFX–ZFYReal time PCRNeotropical canid
spellingShingle Mannise, N.
Trovati, R. G.
Duarte, J. M. B.
Maldonado, J. E.
González, S.
Using non–invasive genetic techniques to assist in maned wolf conservation in a remnant fragment of the Brazilian Cerrado
Animal Biodiversity and Conservation
Fecal DNA
Microsatellite loci
ZFX–ZFY
Real time PCR
Neotropical canid
title Using non–invasive genetic techniques to assist in maned wolf conservation in a remnant fragment of the Brazilian Cerrado
title_full Using non–invasive genetic techniques to assist in maned wolf conservation in a remnant fragment of the Brazilian Cerrado
title_fullStr Using non–invasive genetic techniques to assist in maned wolf conservation in a remnant fragment of the Brazilian Cerrado
title_full_unstemmed Using non–invasive genetic techniques to assist in maned wolf conservation in a remnant fragment of the Brazilian Cerrado
title_short Using non–invasive genetic techniques to assist in maned wolf conservation in a remnant fragment of the Brazilian Cerrado
title_sort using non invasive genetic techniques to assist in maned wolf conservation in a remnant fragment of the brazilian cerrado
topic Fecal DNA
Microsatellite loci
ZFX–ZFY
Real time PCR
Neotropical canid
url http://abc.museucienciesjournals.cat/files/ABC_41-2_pp_315-319-color.pdf
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