Analysis of Bacteriophages with Insulator-Based Dielectrophoresis
Bacterial viruses or phages have great potential in the medical and agricultural fields as alternatives to antibiotics to control nuisance populations of pathogenic bacteria. However, current analysis and purification protocols for phages tend to be resource intensive and have numbers of limitations...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-07-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/10/7/450 |
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| author | Adriana Coll De Peña Nurul Humaira Mohd Redzuan Milky K. Abajorga Nicole Hill Julie A. Thomas Blanca H. Lapizco-Encinas |
| author_facet | Adriana Coll De Peña Nurul Humaira Mohd Redzuan Milky K. Abajorga Nicole Hill Julie A. Thomas Blanca H. Lapizco-Encinas |
| author_sort | Adriana Coll De Peña |
| collection | DOAJ |
| description | Bacterial viruses or phages have great potential in the medical and agricultural fields as alternatives to antibiotics to control nuisance populations of pathogenic bacteria. However, current analysis and purification protocols for phages tend to be resource intensive and have numbers of limitations, such as impacting phage viability. The present study explores the potential of employing the electrokinetic technique of insulator-based dielectrophoresis (iDEP) for virus assessment, separation and enrichment. In particular, the application of the parameter “trapping value” (<i>Tv</i>) is explored as a standardized iDEP signature for each phage species. The present study includes mathematical modeling with COMSOL Multiphysics and extensive experimentation. Three related, but genetically and structurally distinct, phages were studied: <i>Salmonella enterica</i> phage SPN3US, <i>Pseudomonas aeruginosa</i> phage ϕKZ and <i>P. chlororaphis</i> phage 201ϕ2-1. This is the first iDEP study on bacteriophages with large and complex virions and the results illustrate their virions can be successfully enriched with iDEP systems and still retain infectivity. In addition, our results indicate that characterization of the negative dielectrophoretic response of a phage in terms of <i>Tv</i> could be used for predicting individual virus behavior in iDEP systems. The findings reported here can contribute to the establishment of protocols to analyze, purify and/or enrich samples of known and unknown phages. |
| first_indexed | 2024-04-12T00:37:18Z |
| format | Article |
| id | doaj.art-4e396cb6ad2e490ab0b59f0540cdcbe1 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-04-12T00:37:18Z |
| publishDate | 2019-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-4e396cb6ad2e490ab0b59f0540cdcbe12022-12-22T03:55:08ZengMDPI AGMicromachines2072-666X2019-07-0110745010.3390/mi10070450mi10070450Analysis of Bacteriophages with Insulator-Based DielectrophoresisAdriana Coll De Peña0Nurul Humaira Mohd Redzuan1Milky K. Abajorga2Nicole Hill3Julie A. Thomas4Blanca H. Lapizco-Encinas5Microscale Bioseparations Laboratory and Biomedical Engineering Department, Rochester Institute of Technology, Rochester, NY 14623, USAThomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester, NY 14623, USAThomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester, NY 14623, USAMicroscale Bioseparations Laboratory and Biomedical Engineering Department, Rochester Institute of Technology, Rochester, NY 14623, USAThomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester, NY 14623, USAMicroscale Bioseparations Laboratory and Biomedical Engineering Department, Rochester Institute of Technology, Rochester, NY 14623, USABacterial viruses or phages have great potential in the medical and agricultural fields as alternatives to antibiotics to control nuisance populations of pathogenic bacteria. However, current analysis and purification protocols for phages tend to be resource intensive and have numbers of limitations, such as impacting phage viability. The present study explores the potential of employing the electrokinetic technique of insulator-based dielectrophoresis (iDEP) for virus assessment, separation and enrichment. In particular, the application of the parameter “trapping value” (<i>Tv</i>) is explored as a standardized iDEP signature for each phage species. The present study includes mathematical modeling with COMSOL Multiphysics and extensive experimentation. Three related, but genetically and structurally distinct, phages were studied: <i>Salmonella enterica</i> phage SPN3US, <i>Pseudomonas aeruginosa</i> phage ϕKZ and <i>P. chlororaphis</i> phage 201ϕ2-1. This is the first iDEP study on bacteriophages with large and complex virions and the results illustrate their virions can be successfully enriched with iDEP systems and still retain infectivity. In addition, our results indicate that characterization of the negative dielectrophoretic response of a phage in terms of <i>Tv</i> could be used for predicting individual virus behavior in iDEP systems. The findings reported here can contribute to the establishment of protocols to analyze, purify and/or enrich samples of known and unknown phages.https://www.mdpi.com/2072-666X/10/7/450bacteriophagedielectrophoresiselectric fieldelectrophoresiselectrokineticsvirus |
| spellingShingle | Adriana Coll De Peña Nurul Humaira Mohd Redzuan Milky K. Abajorga Nicole Hill Julie A. Thomas Blanca H. Lapizco-Encinas Analysis of Bacteriophages with Insulator-Based Dielectrophoresis Micromachines bacteriophage dielectrophoresis electric field electrophoresis electrokinetics virus |
| title | Analysis of Bacteriophages with Insulator-Based Dielectrophoresis |
| title_full | Analysis of Bacteriophages with Insulator-Based Dielectrophoresis |
| title_fullStr | Analysis of Bacteriophages with Insulator-Based Dielectrophoresis |
| title_full_unstemmed | Analysis of Bacteriophages with Insulator-Based Dielectrophoresis |
| title_short | Analysis of Bacteriophages with Insulator-Based Dielectrophoresis |
| title_sort | analysis of bacteriophages with insulator based dielectrophoresis |
| topic | bacteriophage dielectrophoresis electric field electrophoresis electrokinetics virus |
| url | https://www.mdpi.com/2072-666X/10/7/450 |
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