Significance of survivin immunoreactivity and morphometric analysis of HPV-induced cervical dysplasia
Genomic integration of high-risk human papilloma virus in the nucleus of cervical epithelial mucosal cells leads to epithelial dysplasia. The aim of this study was to determine the relevance of correlation between epithelial survivin expression and the degree of human papilloma virus (HPV)-...
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2014-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2014/0354-46641401429V.pdf |
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| author | Vukomanović-Đurđević Biserka Basta-Jovanović Gordana Baletić N. Berisavac Milica Nenadić D. Perić A. |
| author_facet | Vukomanović-Đurđević Biserka Basta-Jovanović Gordana Baletić N. Berisavac Milica Nenadić D. Perić A. |
| author_sort | Vukomanović-Đurđević Biserka |
| collection | DOAJ |
| description | Genomic integration of high-risk human papilloma virus in the nucleus of
cervical epithelial mucosal cells leads to epithelial dysplasia. The aim of
this study was to determine the relevance of correlation between epithelial
survivin expression and the degree of human papilloma virus (HPV)-induced
cervical epithelial dysplasia, and to establish the significance of
morphometric analysis of the nuclear area in the assessment of the degree of
cervical dysplasia. This retrospective study included 99 women with primary,
previously untreated lesions, and colposcopic findings indicating dysplasia,
in whom a cytological test by Papanicolaou method was interpreted according
to the Bethesda criteria as lowgrade squamous intraepithelial lesion (LSIL),
high-grade squamous intraepithelial lesion (HSIL), and atypical squamous
cells of undetermined significance (ASCUS). We performed human papilloma
virus (HPV) typing by PCR for evidence of viruse types 16, 18, 31, 33. After
biopsy of the cervical mucosa, we performed hematoxylin-eosin (H-E) and
Periodic Acid Schiff (PAS) staining, and immunohistochemical and morphometric
analysis of tissue samples. The control group consisted of 12 women without
dysplasia and without a verified infection of cervical high-risk HPV. A high
statistical correlation between the degree of dysplasia and expression of
survivin was found in patients with different types of cervical dysplasia (p
= 0.003). We observed a high statistical difference between the area of
nuclei at different degrees of cervical dysplasias (p = 0.000). The
high-grade cervical dysplasia had a more than 2-fold higher level of ranking
in comparison to low-grade dysplasia, and a more than 10-fold higher ranking
than the control group without cervical dysplasia. |
| first_indexed | 2024-04-12T23:06:54Z |
| format | Article |
| id | doaj.art-4e3b408f6dd64b9d979a7fc4c346e124 |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-04-12T23:06:54Z |
| publishDate | 2014-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-4e3b408f6dd64b9d979a7fc4c346e1242022-12-22T03:12:53ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392014-01-0166142943610.2298/ABS1401429V0354-46641401429VSignificance of survivin immunoreactivity and morphometric analysis of HPV-induced cervical dysplasiaVukomanović-Đurđević Biserka0Basta-Jovanović Gordana1Baletić N.2Berisavac Milica3Nenadić D.4Perić A.5Institute of Pathology, Faculty of Medicine, Military Medical Academy, BelgradeFaculty of Medicine, BelgradeDepartment of Otorhinolaryngology, Faculty of Medicine, Military Medical Academy, BelgradeFaculty of Medicine, BelgradeDepartment of Gynecology, Faculty of Medicine, Military Medical Academy, BelgradeDepartment of Otorhinolaryngology, Faculty of Medicine, Military Medical Academy, BelgradeGenomic integration of high-risk human papilloma virus in the nucleus of cervical epithelial mucosal cells leads to epithelial dysplasia. The aim of this study was to determine the relevance of correlation between epithelial survivin expression and the degree of human papilloma virus (HPV)-induced cervical epithelial dysplasia, and to establish the significance of morphometric analysis of the nuclear area in the assessment of the degree of cervical dysplasia. This retrospective study included 99 women with primary, previously untreated lesions, and colposcopic findings indicating dysplasia, in whom a cytological test by Papanicolaou method was interpreted according to the Bethesda criteria as lowgrade squamous intraepithelial lesion (LSIL), high-grade squamous intraepithelial lesion (HSIL), and atypical squamous cells of undetermined significance (ASCUS). We performed human papilloma virus (HPV) typing by PCR for evidence of viruse types 16, 18, 31, 33. After biopsy of the cervical mucosa, we performed hematoxylin-eosin (H-E) and Periodic Acid Schiff (PAS) staining, and immunohistochemical and morphometric analysis of tissue samples. The control group consisted of 12 women without dysplasia and without a verified infection of cervical high-risk HPV. A high statistical correlation between the degree of dysplasia and expression of survivin was found in patients with different types of cervical dysplasia (p = 0.003). We observed a high statistical difference between the area of nuclei at different degrees of cervical dysplasias (p = 0.000). The high-grade cervical dysplasia had a more than 2-fold higher level of ranking in comparison to low-grade dysplasia, and a more than 10-fold higher ranking than the control group without cervical dysplasia.http://www.doiserbia.nb.rs/img/doi/0354-4664/2014/0354-46641401429V.pdfCervical dysplasiahuman papilloma viruses (HPV)morphometric analysissurvivin |
| spellingShingle | Vukomanović-Đurđević Biserka Basta-Jovanović Gordana Baletić N. Berisavac Milica Nenadić D. Perić A. Significance of survivin immunoreactivity and morphometric analysis of HPV-induced cervical dysplasia Archives of Biological Sciences Cervical dysplasia human papilloma viruses (HPV) morphometric analysis survivin |
| title | Significance of survivin immunoreactivity and morphometric analysis of HPV-induced cervical dysplasia |
| title_full | Significance of survivin immunoreactivity and morphometric analysis of HPV-induced cervical dysplasia |
| title_fullStr | Significance of survivin immunoreactivity and morphometric analysis of HPV-induced cervical dysplasia |
| title_full_unstemmed | Significance of survivin immunoreactivity and morphometric analysis of HPV-induced cervical dysplasia |
| title_short | Significance of survivin immunoreactivity and morphometric analysis of HPV-induced cervical dysplasia |
| title_sort | significance of survivin immunoreactivity and morphometric analysis of hpv induced cervical dysplasia |
| topic | Cervical dysplasia human papilloma viruses (HPV) morphometric analysis survivin |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2014/0354-46641401429V.pdf |
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