Region-specific regulation of stem cell-driven regeneration in tapeworms
Tapeworms grow at rates rivaling the fastest-growing metazoan tissues. To propagate they shed large parts of their body; to replace these lost tissues they regenerate proglottids (segments) as part of normal homeostasis. Their remarkable growth and regeneration are fueled by adult somatic stem cells...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2019-09-01
|
| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/48958 |
| _version_ | 1811235970294480896 |
|---|---|
| author | Tania Rozario Edward B Quinn Jianbin Wang Richard E Davis Phillip A Newmark |
| author_facet | Tania Rozario Edward B Quinn Jianbin Wang Richard E Davis Phillip A Newmark |
| author_sort | Tania Rozario |
| collection | DOAJ |
| description | Tapeworms grow at rates rivaling the fastest-growing metazoan tissues. To propagate they shed large parts of their body; to replace these lost tissues they regenerate proglottids (segments) as part of normal homeostasis. Their remarkable growth and regeneration are fueled by adult somatic stem cells that have yet to be characterized molecularly. Using the rat intestinal tapeworm, Hymenolepis diminuta, we find that regenerative potential is regionally limited to the neck, where head-dependent extrinsic signals create a permissive microenvironment for stem cell-driven regeneration. Using transcriptomic analyses and RNA interference, we characterize and functionally validate regulators of tapeworm growth and regeneration. We find no evidence that stem cells are restricted to the regeneration-competent neck. Instead, lethally irradiated tapeworms can be rescued when cells from either regeneration-competent or regeneration-incompetent regions are transplanted into the neck. Together, the head and neck tissues provide extrinsic cues that regulate stem cells, enabling region-specific regeneration in this parasite. |
| first_indexed | 2024-04-12T12:01:14Z |
| format | Article |
| id | doaj.art-4e42654e7ffe452ebabe5752e4b98af0 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T12:01:14Z |
| publishDate | 2019-09-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-4e42654e7ffe452ebabe5752e4b98af02022-12-22T03:33:51ZengeLife Sciences Publications LtdeLife2050-084X2019-09-01810.7554/eLife.48958Region-specific regulation of stem cell-driven regeneration in tapewormsTania Rozario0https://orcid.org/0000-0002-9971-5211Edward B Quinn1Jianbin Wang2https://orcid.org/0000-0003-3155-894XRichard E Davis3Phillip A Newmark4https://orcid.org/0000-0003-0793-022XMorgridge Institute for Research, Madison, United StatesMorgridge Institute for Research, Madison, United StatesRNA Bioscience Initiative, Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, United StatesRNA Bioscience Initiative, Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, United StatesMorgridge Institute for Research, Madison, United States; Howard Hughes Medical Institute, Chevy Chase, United States; Department of Integrative Biology, University of Wisconsin–Madison, Madison, United StatesTapeworms grow at rates rivaling the fastest-growing metazoan tissues. To propagate they shed large parts of their body; to replace these lost tissues they regenerate proglottids (segments) as part of normal homeostasis. Their remarkable growth and regeneration are fueled by adult somatic stem cells that have yet to be characterized molecularly. Using the rat intestinal tapeworm, Hymenolepis diminuta, we find that regenerative potential is regionally limited to the neck, where head-dependent extrinsic signals create a permissive microenvironment for stem cell-driven regeneration. Using transcriptomic analyses and RNA interference, we characterize and functionally validate regulators of tapeworm growth and regeneration. We find no evidence that stem cells are restricted to the regeneration-competent neck. Instead, lethally irradiated tapeworms can be rescued when cells from either regeneration-competent or regeneration-incompetent regions are transplanted into the neck. Together, the head and neck tissues provide extrinsic cues that regulate stem cells, enabling region-specific regeneration in this parasite.https://elifesciences.org/articles/48958tapewormHymenolepis diminutacestodeflatwormstem cells |
| spellingShingle | Tania Rozario Edward B Quinn Jianbin Wang Richard E Davis Phillip A Newmark Region-specific regulation of stem cell-driven regeneration in tapeworms eLife tapeworm Hymenolepis diminuta cestode flatworm stem cells |
| title | Region-specific regulation of stem cell-driven regeneration in tapeworms |
| title_full | Region-specific regulation of stem cell-driven regeneration in tapeworms |
| title_fullStr | Region-specific regulation of stem cell-driven regeneration in tapeworms |
| title_full_unstemmed | Region-specific regulation of stem cell-driven regeneration in tapeworms |
| title_short | Region-specific regulation of stem cell-driven regeneration in tapeworms |
| title_sort | region specific regulation of stem cell driven regeneration in tapeworms |
| topic | tapeworm Hymenolepis diminuta cestode flatworm stem cells |
| url | https://elifesciences.org/articles/48958 |
| work_keys_str_mv | AT taniarozario regionspecificregulationofstemcelldrivenregenerationintapeworms AT edwardbquinn regionspecificregulationofstemcelldrivenregenerationintapeworms AT jianbinwang regionspecificregulationofstemcelldrivenregenerationintapeworms AT richardedavis regionspecificregulationofstemcelldrivenregenerationintapeworms AT phillipanewmark regionspecificregulationofstemcelldrivenregenerationintapeworms |