The Cellular Proteins Grb2 and DDX3 Are Increased upon Human Cytomegalovirus Infection and Act in a Proviral Fashion.
While it is well established that human cytomegalovirus (HCMV) upregulates many cellular proteins and incorporates several of them into its virion, little is known about the functional relevance of such virus-host interactions. Two cellular proteins, Grb2 and DDX3, gained our interest as they appear...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4509573?pdf=render |
| _version_ | 1818311167053922304 |
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| author | Yolaine Cavignac Diana Lieber Kerstin Laib Sampaio Johannes Madlung Tobias Lamkemeyer Gerhard Jahn Alfred Nordheim Christian Sinzger |
| author_facet | Yolaine Cavignac Diana Lieber Kerstin Laib Sampaio Johannes Madlung Tobias Lamkemeyer Gerhard Jahn Alfred Nordheim Christian Sinzger |
| author_sort | Yolaine Cavignac |
| collection | DOAJ |
| description | While it is well established that human cytomegalovirus (HCMV) upregulates many cellular proteins and incorporates several of them into its virion, little is known about the functional relevance of such virus-host interactions. Two cellular proteins, Grb2 and DDX3, gained our interest as they appeared enriched in virion particles and this incorporation depended on the viral tegument protein pp65, suggesting a functional relevance. We therefore tested whether the level of these proteins is altered upon HCMV infection and whether they support viral replication. Immunoblotting analyses of cellular fractions showed increased levels of both proteins in infected cells with a maximum at 2 d p.i. and a reduction of the soluble Grb2 fraction. Knockdown of either gene by transfection of siRNAs reduced viral spread not only of the cell culture adapted HCMV strain TB40/E but also of recent clinical isolates. Apparently, Grb2 and DDX3 are proviral cellular factors that are upregulated in infected cells. |
| first_indexed | 2024-12-13T07:57:39Z |
| format | Article |
| id | doaj.art-4e4287a5a0b84174a4b2b20959f14b11 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T07:57:39Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-4e4287a5a0b84174a4b2b20959f14b112022-12-21T23:54:30ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01106e013161410.1371/journal.pone.0131614The Cellular Proteins Grb2 and DDX3 Are Increased upon Human Cytomegalovirus Infection and Act in a Proviral Fashion.Yolaine CavignacDiana LieberKerstin Laib SampaioJohannes MadlungTobias LamkemeyerGerhard JahnAlfred NordheimChristian SinzgerWhile it is well established that human cytomegalovirus (HCMV) upregulates many cellular proteins and incorporates several of them into its virion, little is known about the functional relevance of such virus-host interactions. Two cellular proteins, Grb2 and DDX3, gained our interest as they appeared enriched in virion particles and this incorporation depended on the viral tegument protein pp65, suggesting a functional relevance. We therefore tested whether the level of these proteins is altered upon HCMV infection and whether they support viral replication. Immunoblotting analyses of cellular fractions showed increased levels of both proteins in infected cells with a maximum at 2 d p.i. and a reduction of the soluble Grb2 fraction. Knockdown of either gene by transfection of siRNAs reduced viral spread not only of the cell culture adapted HCMV strain TB40/E but also of recent clinical isolates. Apparently, Grb2 and DDX3 are proviral cellular factors that are upregulated in infected cells.http://europepmc.org/articles/PMC4509573?pdf=render |
| spellingShingle | Yolaine Cavignac Diana Lieber Kerstin Laib Sampaio Johannes Madlung Tobias Lamkemeyer Gerhard Jahn Alfred Nordheim Christian Sinzger The Cellular Proteins Grb2 and DDX3 Are Increased upon Human Cytomegalovirus Infection and Act in a Proviral Fashion. PLoS ONE |
| title | The Cellular Proteins Grb2 and DDX3 Are Increased upon Human Cytomegalovirus Infection and Act in a Proviral Fashion. |
| title_full | The Cellular Proteins Grb2 and DDX3 Are Increased upon Human Cytomegalovirus Infection and Act in a Proviral Fashion. |
| title_fullStr | The Cellular Proteins Grb2 and DDX3 Are Increased upon Human Cytomegalovirus Infection and Act in a Proviral Fashion. |
| title_full_unstemmed | The Cellular Proteins Grb2 and DDX3 Are Increased upon Human Cytomegalovirus Infection and Act in a Proviral Fashion. |
| title_short | The Cellular Proteins Grb2 and DDX3 Are Increased upon Human Cytomegalovirus Infection and Act in a Proviral Fashion. |
| title_sort | cellular proteins grb2 and ddx3 are increased upon human cytomegalovirus infection and act in a proviral fashion |
| url | http://europepmc.org/articles/PMC4509573?pdf=render |
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