Sensorless Wavefront Correction in Two-Photon Microscopy Across Different Turbidity Scales
Adaptive optics (AO) is a powerful tool to increase the imaging depth of multiphoton scanning microscopes. For highly scattering tissues, sensorless wavefront correction techniques exhibit robust performance and present a straight-forward implementation of AO. However, for many applications such as...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Physics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphy.2022.884053/full |
| _version_ | 1797741679143288832 |
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| author | Maximilian Sohmen Molly A. May Nicolas Barré Monika Ritsch-Marte Alexander Jesacher |
| author_facet | Maximilian Sohmen Molly A. May Nicolas Barré Monika Ritsch-Marte Alexander Jesacher |
| author_sort | Maximilian Sohmen |
| collection | DOAJ |
| description | Adaptive optics (AO) is a powerful tool to increase the imaging depth of multiphoton scanning microscopes. For highly scattering tissues, sensorless wavefront correction techniques exhibit robust performance and present a straight-forward implementation of AO. However, for many applications such as live-tissue imaging, the speed of aberration correction remains a critical bottleneck. Dynamic Adaptive Scattering compensation Holography (DASH)—a fast-converging sensorless AO technique introduced recently for scatter compensation in nonlinear scanning microscopy—addresses this issue. DASH has been targeted at highly turbid media, but to-date it has remained an open question how it performs for mild turbidity, where limitations imposed by phase-only wavefront shaping are expected to impede its convergence. In this work, we study the performance of DASH across different turbidity regimes, in simulation as well as experiments. We further provide a direct comparison between DASH and a novel, modified version of the Continuous Sequential Algorithm (CSA) which we call Amplified CSA (a-CSA). |
| first_indexed | 2024-03-12T14:30:12Z |
| format | Article |
| id | doaj.art-4e432db4ca6745049927efda4477a635 |
| institution | Directory Open Access Journal |
| issn | 2296-424X |
| language | English |
| last_indexed | 2024-03-12T14:30:12Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physics |
| spelling | doaj.art-4e432db4ca6745049927efda4477a6352023-08-17T14:19:50ZengFrontiers Media S.A.Frontiers in Physics2296-424X2022-05-011010.3389/fphy.2022.884053884053Sensorless Wavefront Correction in Two-Photon Microscopy Across Different Turbidity ScalesMaximilian SohmenMolly A. MayNicolas BarréMonika Ritsch-MarteAlexander JesacherAdaptive optics (AO) is a powerful tool to increase the imaging depth of multiphoton scanning microscopes. For highly scattering tissues, sensorless wavefront correction techniques exhibit robust performance and present a straight-forward implementation of AO. However, for many applications such as live-tissue imaging, the speed of aberration correction remains a critical bottleneck. Dynamic Adaptive Scattering compensation Holography (DASH)—a fast-converging sensorless AO technique introduced recently for scatter compensation in nonlinear scanning microscopy—addresses this issue. DASH has been targeted at highly turbid media, but to-date it has remained an open question how it performs for mild turbidity, where limitations imposed by phase-only wavefront shaping are expected to impede its convergence. In this work, we study the performance of DASH across different turbidity regimes, in simulation as well as experiments. We further provide a direct comparison between DASH and a novel, modified version of the Continuous Sequential Algorithm (CSA) which we call Amplified CSA (a-CSA).https://www.frontiersin.org/articles/10.3389/fphy.2022.884053/fullmultiphoton microscopyadaptive opticsscatter compensationwavefront sensingbrain imagingaberration and wavefront analysis |
| spellingShingle | Maximilian Sohmen Molly A. May Nicolas Barré Monika Ritsch-Marte Alexander Jesacher Sensorless Wavefront Correction in Two-Photon Microscopy Across Different Turbidity Scales Frontiers in Physics multiphoton microscopy adaptive optics scatter compensation wavefront sensing brain imaging aberration and wavefront analysis |
| title | Sensorless Wavefront Correction in Two-Photon Microscopy Across Different Turbidity Scales |
| title_full | Sensorless Wavefront Correction in Two-Photon Microscopy Across Different Turbidity Scales |
| title_fullStr | Sensorless Wavefront Correction in Two-Photon Microscopy Across Different Turbidity Scales |
| title_full_unstemmed | Sensorless Wavefront Correction in Two-Photon Microscopy Across Different Turbidity Scales |
| title_short | Sensorless Wavefront Correction in Two-Photon Microscopy Across Different Turbidity Scales |
| title_sort | sensorless wavefront correction in two photon microscopy across different turbidity scales |
| topic | multiphoton microscopy adaptive optics scatter compensation wavefront sensing brain imaging aberration and wavefront analysis |
| url | https://www.frontiersin.org/articles/10.3389/fphy.2022.884053/full |
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