Recruitment of Reverse Transcriptase-Cas1 Fusion Proteins by Type VI-A CRISPR-Cas Systems
Type VI CRISPR–Cas systems contain a single effector nuclease (Cas13) that exclusively targets single-stranded RNA. It remains unknown how these systems acquire spacers. It has been suggested that type VI systems with adaptation modules can acquire spacers from RNA bacteriophages, but sequence simil...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-09-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.02160/full |
| _version_ | 1818247471569043456 |
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| author | Nicolás Toro Mario Rodríguez Mestre Francisco Martínez-Abarca Alejandro González-Delgado |
| author_facet | Nicolás Toro Mario Rodríguez Mestre Francisco Martínez-Abarca Alejandro González-Delgado |
| author_sort | Nicolás Toro |
| collection | DOAJ |
| description | Type VI CRISPR–Cas systems contain a single effector nuclease (Cas13) that exclusively targets single-stranded RNA. It remains unknown how these systems acquire spacers. It has been suggested that type VI systems with adaptation modules can acquire spacers from RNA bacteriophages, but sequence similarities suggest that spacers may provide immunity to DNA phages. We searched databases for Cas13 proteins with linked RTs. We identified two different type VI-A systems with adaptation modules including an RT-Cas1 fusion and Cas2 proteins. Phylogenetic reconstruction analyses revealed that these adaptation modules were recruited by different effector Cas13a proteins, possibly from RT-associated type III-D systems within the bacterial classes Alphaproteobacteria and Clostridia. These type VI-A systems are predicted to acquire spacers from RNA molecules, paving the way for future studies investigating their role in bacterial adaptive immunity and biotechnological applications. |
| first_indexed | 2024-12-12T15:05:14Z |
| format | Article |
| id | doaj.art-4e479d69cb9c49e59aabe11011059a98 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-12T15:05:14Z |
| publishDate | 2019-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-4e479d69cb9c49e59aabe11011059a982022-12-22T00:20:45ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-09-011010.3389/fmicb.2019.02160484598Recruitment of Reverse Transcriptase-Cas1 Fusion Proteins by Type VI-A CRISPR-Cas SystemsNicolás ToroMario Rodríguez MestreFrancisco Martínez-AbarcaAlejandro González-DelgadoType VI CRISPR–Cas systems contain a single effector nuclease (Cas13) that exclusively targets single-stranded RNA. It remains unknown how these systems acquire spacers. It has been suggested that type VI systems with adaptation modules can acquire spacers from RNA bacteriophages, but sequence similarities suggest that spacers may provide immunity to DNA phages. We searched databases for Cas13 proteins with linked RTs. We identified two different type VI-A systems with adaptation modules including an RT-Cas1 fusion and Cas2 proteins. Phylogenetic reconstruction analyses revealed that these adaptation modules were recruited by different effector Cas13a proteins, possibly from RT-associated type III-D systems within the bacterial classes Alphaproteobacteria and Clostridia. These type VI-A systems are predicted to acquire spacers from RNA molecules, paving the way for future studies investigating their role in bacterial adaptive immunity and biotechnological applications.https://www.frontiersin.org/article/10.3389/fmicb.2019.02160/fullreverse transcriptaseCRISPR-Casphylogenytype VI CRISPRCas1 |
| spellingShingle | Nicolás Toro Mario Rodríguez Mestre Francisco Martínez-Abarca Alejandro González-Delgado Recruitment of Reverse Transcriptase-Cas1 Fusion Proteins by Type VI-A CRISPR-Cas Systems Frontiers in Microbiology reverse transcriptase CRISPR-Cas phylogeny type VI CRISPR Cas1 |
| title | Recruitment of Reverse Transcriptase-Cas1 Fusion Proteins by Type VI-A CRISPR-Cas Systems |
| title_full | Recruitment of Reverse Transcriptase-Cas1 Fusion Proteins by Type VI-A CRISPR-Cas Systems |
| title_fullStr | Recruitment of Reverse Transcriptase-Cas1 Fusion Proteins by Type VI-A CRISPR-Cas Systems |
| title_full_unstemmed | Recruitment of Reverse Transcriptase-Cas1 Fusion Proteins by Type VI-A CRISPR-Cas Systems |
| title_short | Recruitment of Reverse Transcriptase-Cas1 Fusion Proteins by Type VI-A CRISPR-Cas Systems |
| title_sort | recruitment of reverse transcriptase cas1 fusion proteins by type vi a crispr cas systems |
| topic | reverse transcriptase CRISPR-Cas phylogeny type VI CRISPR Cas1 |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.02160/full |
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