Auxin and carbohydrate control flower bud development in Anthurium andraeanum during early stage of sexual reproduction
Abstract Background Flower buds of Anthurium andraeanum frequently cease to grow and abort during the early flowering stage, resulting in prolonged planting times and increased commercialization costs. Nevertheless, limited knowledge exists of the mechanism of flower development after initiation in...
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| Format: | Article |
| Language: | English |
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BMC
2024-03-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-024-04869-0 |
| _version_ | 1797275277027442688 |
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| author | Xiao Wan Long-Hai Zou Xiaoyun Pan Yaying Ge Liang Jin Qunyang Cao Jiewei Shi Danqing Tian |
| author_facet | Xiao Wan Long-Hai Zou Xiaoyun Pan Yaying Ge Liang Jin Qunyang Cao Jiewei Shi Danqing Tian |
| author_sort | Xiao Wan |
| collection | DOAJ |
| description | Abstract Background Flower buds of Anthurium andraeanum frequently cease to grow and abort during the early flowering stage, resulting in prolonged planting times and increased commercialization costs. Nevertheless, limited knowledge exists of the mechanism of flower development after initiation in A. andraeanum. Results In this study, the measurement of carbohydrate flow and intensity between leaves and flowers during different growth stages showed that tender leaves are strong sinks and their concomitant flowers are weak ones. This suggested that the tender leaves compete with their concomitant flower buds for carbohydrates during the early growth stages, potentially causing the abortion of the flower buds. The analysis of transcriptomic differentially expressed genes suggested that genes related to sucrose metabolism and auxin response play an important role during flower bud development. Particularly, co-expression network analysis found that AaSPL12 is a hub gene engaged in flower development by collaborating carbohydrate and auxin signals. Yeast Two Hybrid assays revealed that AaSPL12 can interact with AaARP, a protein that serves as an indicator of dormancy. Additionally, the application of exogenous IAA and sucrose can suppress the expression of AaARP, augment the transcriptional abundance of AaSPL12, and consequently expedite flower development in Anthurium andraeanum. Conclusions Collectively, our findings indicated that the combination of auxin and sugar signals could potentially suppress the repression of AaARP protein to AaSPL12, thus advancing the development of flower buds in Anthurium andraeanum. |
| first_indexed | 2024-03-07T15:10:59Z |
| format | Article |
| id | doaj.art-4e4965ad57544893bd0c87fb8db9346e |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-03-07T15:10:59Z |
| publishDate | 2024-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-4e4965ad57544893bd0c87fb8db9346e2024-03-05T18:37:32ZengBMCBMC Plant Biology1471-22292024-03-0124111310.1186/s12870-024-04869-0Auxin and carbohydrate control flower bud development in Anthurium andraeanum during early stage of sexual reproductionXiao Wan0Long-Hai Zou1Xiaoyun Pan2Yaying Ge3Liang Jin4Qunyang Cao5Jiewei Shi6Danqing Tian7Zhejiang Institute of Landscape Plants and Flowers, Zhejiang Academy of Agricultural SciencesState Key Laboratory of Subtropical Silviculture, Bamboo Industry Institute, Zhejiang A&F UniversityZhejiang Institute of Landscape Plants and Flowers, Zhejiang Academy of Agricultural SciencesZhejiang Institute of Landscape Plants and Flowers, Zhejiang Academy of Agricultural SciencesZhejiang Institute of Landscape Plants and Flowers, Zhejiang Academy of Agricultural SciencesZhejiang Institute of Landscape Plants and Flowers, Zhejiang Academy of Agricultural SciencesZhejiang Institute of Landscape Plants and Flowers, Zhejiang Academy of Agricultural SciencesZhejiang Institute of Landscape Plants and Flowers, Zhejiang Academy of Agricultural SciencesAbstract Background Flower buds of Anthurium andraeanum frequently cease to grow and abort during the early flowering stage, resulting in prolonged planting times and increased commercialization costs. Nevertheless, limited knowledge exists of the mechanism of flower development after initiation in A. andraeanum. Results In this study, the measurement of carbohydrate flow and intensity between leaves and flowers during different growth stages showed that tender leaves are strong sinks and their concomitant flowers are weak ones. This suggested that the tender leaves compete with their concomitant flower buds for carbohydrates during the early growth stages, potentially causing the abortion of the flower buds. The analysis of transcriptomic differentially expressed genes suggested that genes related to sucrose metabolism and auxin response play an important role during flower bud development. Particularly, co-expression network analysis found that AaSPL12 is a hub gene engaged in flower development by collaborating carbohydrate and auxin signals. Yeast Two Hybrid assays revealed that AaSPL12 can interact with AaARP, a protein that serves as an indicator of dormancy. Additionally, the application of exogenous IAA and sucrose can suppress the expression of AaARP, augment the transcriptional abundance of AaSPL12, and consequently expedite flower development in Anthurium andraeanum. Conclusions Collectively, our findings indicated that the combination of auxin and sugar signals could potentially suppress the repression of AaARP protein to AaSPL12, thus advancing the development of flower buds in Anthurium andraeanum.https://doi.org/10.1186/s12870-024-04869-0Anthurium andraeanumARPAuxinCarbohydrateFloral bud developmentSPL12 |
| spellingShingle | Xiao Wan Long-Hai Zou Xiaoyun Pan Yaying Ge Liang Jin Qunyang Cao Jiewei Shi Danqing Tian Auxin and carbohydrate control flower bud development in Anthurium andraeanum during early stage of sexual reproduction BMC Plant Biology Anthurium andraeanum ARP Auxin Carbohydrate Floral bud development SPL12 |
| title | Auxin and carbohydrate control flower bud development in Anthurium andraeanum during early stage of sexual reproduction |
| title_full | Auxin and carbohydrate control flower bud development in Anthurium andraeanum during early stage of sexual reproduction |
| title_fullStr | Auxin and carbohydrate control flower bud development in Anthurium andraeanum during early stage of sexual reproduction |
| title_full_unstemmed | Auxin and carbohydrate control flower bud development in Anthurium andraeanum during early stage of sexual reproduction |
| title_short | Auxin and carbohydrate control flower bud development in Anthurium andraeanum during early stage of sexual reproduction |
| title_sort | auxin and carbohydrate control flower bud development in anthurium andraeanum during early stage of sexual reproduction |
| topic | Anthurium andraeanum ARP Auxin Carbohydrate Floral bud development SPL12 |
| url | https://doi.org/10.1186/s12870-024-04869-0 |
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