Spectrophotometric Determination of Chlordiazepoxide in Pharmaceutical Formulations via Oxidative Coupling Reaction with Phenothiazine
Abstract A sensitive, precise and reliable indirect spectrophotometric method for the determination of chlordiazepoxide (CDE) in pure and pharmaceutical dosage forms is described. The method is based on oxidative coupling reaction between amino group resulting from acidic decomposition of CDE wi...
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| Format: | Article |
| Language: | English |
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College of Pharmacy University of Baghdad
2018-12-01
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| Series: | Iraqi Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | https://bijps.uobaghdad.edu.iq/index.php/bijps/article/view/761 |
| _version_ | 1811208978899664896 |
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| author | Mariam J. Ahmed hind H. Abdullah |
| author_facet | Mariam J. Ahmed hind H. Abdullah |
| author_sort | Mariam J. Ahmed |
| collection | DOAJ |
| description |
Abstract
A sensitive, precise and reliable indirect spectrophotometric method for the determination of chlordiazepoxide (CDE) in pure and pharmaceutical dosage forms is described. The method is based on oxidative coupling reaction between amino group resulting from acidic decomposition of CDE with phenothiazine in the presence of sodium periodate to produce an intense green soluble dye that is stable and shows a maximum absorption at 602 nm. The calibration plot indicates that Beer’s law is obeyed over the concentration range of 0.1?50 µg/mL, with a molar absorptivity of 1×104 L/mol cm and correlation coefficient of 0.9994.All the conditions that affecting on the stability and sensitivity of the formed product were studied and optimized and the suggested method was effectively applied for the determination of CDE in commercial dosage forms.
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| first_indexed | 2024-04-12T04:30:59Z |
| format | Article |
| id | doaj.art-4e4bf3191a704beb87d5bf45ac259cee |
| institution | Directory Open Access Journal |
| issn | 2521-3512 1683-3597 |
| language | English |
| last_indexed | 2024-04-12T04:30:59Z |
| publishDate | 2018-12-01 |
| publisher | College of Pharmacy University of Baghdad |
| record_format | Article |
| series | Iraqi Journal of Pharmaceutical Sciences |
| spelling | doaj.art-4e4bf3191a704beb87d5bf45ac259cee2022-12-22T03:47:55ZengCollege of Pharmacy University of BaghdadIraqi Journal of Pharmaceutical Sciences2521-35121683-35972018-12-0127210.31351/vol27iss2pp7-14761Spectrophotometric Determination of Chlordiazepoxide in Pharmaceutical Formulations via Oxidative Coupling Reaction with PhenothiazineMariam J. Ahmedhind H. Abdullah0university of Baghdad, college of science, chemistry departmewnt Abstract A sensitive, precise and reliable indirect spectrophotometric method for the determination of chlordiazepoxide (CDE) in pure and pharmaceutical dosage forms is described. The method is based on oxidative coupling reaction between amino group resulting from acidic decomposition of CDE with phenothiazine in the presence of sodium periodate to produce an intense green soluble dye that is stable and shows a maximum absorption at 602 nm. The calibration plot indicates that Beer’s law is obeyed over the concentration range of 0.1?50 µg/mL, with a molar absorptivity of 1×104 L/mol cm and correlation coefficient of 0.9994.All the conditions that affecting on the stability and sensitivity of the formed product were studied and optimized and the suggested method was effectively applied for the determination of CDE in commercial dosage forms. https://bijps.uobaghdad.edu.iq/index.php/bijps/article/view/761Chlordiazepoxide, phenothiazine, oxidative coupling reaction. |
| spellingShingle | Mariam J. Ahmed hind H. Abdullah Spectrophotometric Determination of Chlordiazepoxide in Pharmaceutical Formulations via Oxidative Coupling Reaction with Phenothiazine Iraqi Journal of Pharmaceutical Sciences Chlordiazepoxide, phenothiazine, oxidative coupling reaction. |
| title | Spectrophotometric Determination of Chlordiazepoxide in Pharmaceutical Formulations via Oxidative Coupling Reaction with Phenothiazine |
| title_full | Spectrophotometric Determination of Chlordiazepoxide in Pharmaceutical Formulations via Oxidative Coupling Reaction with Phenothiazine |
| title_fullStr | Spectrophotometric Determination of Chlordiazepoxide in Pharmaceutical Formulations via Oxidative Coupling Reaction with Phenothiazine |
| title_full_unstemmed | Spectrophotometric Determination of Chlordiazepoxide in Pharmaceutical Formulations via Oxidative Coupling Reaction with Phenothiazine |
| title_short | Spectrophotometric Determination of Chlordiazepoxide in Pharmaceutical Formulations via Oxidative Coupling Reaction with Phenothiazine |
| title_sort | spectrophotometric determination of chlordiazepoxide in pharmaceutical formulations via oxidative coupling reaction with phenothiazine |
| topic | Chlordiazepoxide, phenothiazine, oxidative coupling reaction. |
| url | https://bijps.uobaghdad.edu.iq/index.php/bijps/article/view/761 |
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