Investigation on the Influence of Production and Incubation Temperature on the Growth, Virulence, Germination, and Conidial Size of <i>Metarhizium brunneum</i> for Granule Development
Important for the infection of an insect with an entomopathogenic fungus and its use as a plant protection agent are its growth, conidiation, germination, and virulence, which all depend on the environmental temperature. We investigated not only the effect of environmental temperature but also that...
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MDPI AG
2023-06-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/9/6/668 |
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| author | Tanja Seib Katharina Fischer Anna Maria Sturm Dietrich Stephan |
| author_facet | Tanja Seib Katharina Fischer Anna Maria Sturm Dietrich Stephan |
| author_sort | Tanja Seib |
| collection | DOAJ |
| description | Important for the infection of an insect with an entomopathogenic fungus and its use as a plant protection agent are its growth, conidiation, germination, and virulence, which all depend on the environmental temperature. We investigated not only the effect of environmental temperature but also that of production temperature of the fungus. For this purpose, <i>Metarhizium brunneum</i> JKI-BI-1450 was produced and incubated at different temperatures, and the factors mentioned as well as conidial size were determined. The temperature at which the fungus was produced affects its subsequent growth and conidiation on granule formulation, the speed of germination, and the conidial width, but not its final germination or virulence. The growth and conidiation was at its highest when the fungus was produced at 25 °C, whereas when the germination was faster, the warmer the fungus was produced. The incubation temperature optimum of JKI-BI-1450 in relation to growth, speed of germination, and survival time was 25–30 °C and for conidiation 20–25 °C. Conidial length decreased with increasing incubation temperature. Although the fungus could not be adapted to unfavorable conditions by the production temperature, it was found that the quality of a biological control agent based on entomopathogenic fungi can be positively influenced by its production temperature. |
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| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-11T02:16:17Z |
| publishDate | 2023-06-01 |
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| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-4e4dfe454870464e83e297c2d11663d12023-11-18T11:09:11ZengMDPI AGJournal of Fungi2309-608X2023-06-019666810.3390/jof9060668Investigation on the Influence of Production and Incubation Temperature on the Growth, Virulence, Germination, and Conidial Size of <i>Metarhizium brunneum</i> for Granule DevelopmentTanja Seib0Katharina Fischer1Anna Maria Sturm2Dietrich Stephan3Julius Kühn-Institute, Federal Research Centre for Cultivated Plants, Institute for Biological Control, Schwabenheimerstraße 101, 69221 Dossenheim, GermanyJulius Kühn-Institute, Federal Research Centre for Cultivated Plants, Institute for Biological Control, Schwabenheimerstraße 101, 69221 Dossenheim, GermanyTechnical University Darmstadt, Department Biologie, Schnittspahnstraße 4, 64287 Darmstadt, GermanyJulius Kühn-Institute, Federal Research Centre for Cultivated Plants, Institute for Biological Control, Schwabenheimerstraße 101, 69221 Dossenheim, GermanyImportant for the infection of an insect with an entomopathogenic fungus and its use as a plant protection agent are its growth, conidiation, germination, and virulence, which all depend on the environmental temperature. We investigated not only the effect of environmental temperature but also that of production temperature of the fungus. For this purpose, <i>Metarhizium brunneum</i> JKI-BI-1450 was produced and incubated at different temperatures, and the factors mentioned as well as conidial size were determined. The temperature at which the fungus was produced affects its subsequent growth and conidiation on granule formulation, the speed of germination, and the conidial width, but not its final germination or virulence. The growth and conidiation was at its highest when the fungus was produced at 25 °C, whereas when the germination was faster, the warmer the fungus was produced. The incubation temperature optimum of JKI-BI-1450 in relation to growth, speed of germination, and survival time was 25–30 °C and for conidiation 20–25 °C. Conidial length decreased with increasing incubation temperature. Although the fungus could not be adapted to unfavorable conditions by the production temperature, it was found that the quality of a biological control agent based on entomopathogenic fungi can be positively influenced by its production temperature.https://www.mdpi.com/2309-608X/9/6/668conidial sizegerminationgranulegrowth<i>Metarhizium brunneum</i>temperature |
| spellingShingle | Tanja Seib Katharina Fischer Anna Maria Sturm Dietrich Stephan Investigation on the Influence of Production and Incubation Temperature on the Growth, Virulence, Germination, and Conidial Size of <i>Metarhizium brunneum</i> for Granule Development Journal of Fungi conidial size germination granule growth <i>Metarhizium brunneum</i> temperature |
| title | Investigation on the Influence of Production and Incubation Temperature on the Growth, Virulence, Germination, and Conidial Size of <i>Metarhizium brunneum</i> for Granule Development |
| title_full | Investigation on the Influence of Production and Incubation Temperature on the Growth, Virulence, Germination, and Conidial Size of <i>Metarhizium brunneum</i> for Granule Development |
| title_fullStr | Investigation on the Influence of Production and Incubation Temperature on the Growth, Virulence, Germination, and Conidial Size of <i>Metarhizium brunneum</i> for Granule Development |
| title_full_unstemmed | Investigation on the Influence of Production and Incubation Temperature on the Growth, Virulence, Germination, and Conidial Size of <i>Metarhizium brunneum</i> for Granule Development |
| title_short | Investigation on the Influence of Production and Incubation Temperature on the Growth, Virulence, Germination, and Conidial Size of <i>Metarhizium brunneum</i> for Granule Development |
| title_sort | investigation on the influence of production and incubation temperature on the growth virulence germination and conidial size of i metarhizium brunneum i for granule development |
| topic | conidial size germination granule growth <i>Metarhizium brunneum</i> temperature |
| url | https://www.mdpi.com/2309-608X/9/6/668 |
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