Antigen induced production of υ-interferon ex vivo, in the peripheral blood of patients with active pulmonary tuberculosis
Tuberculosis (TB) is one of the most significant problems in the Russian Health Care. Russia remains on the list of the 22 countries with a high TB incidence and on the third place in the world with a high prevalence of Drug Resistant TB [1]. It is urgently needed to develop new TB diagnostic method...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | Russian |
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Journal Infectology
2014-09-01
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| Series: | Журнал инфектологии |
| Subjects: | |
| Online Access: | https://journal.niidi.ru/jofin/article/view/164 |
| _version_ | 1797695561361522688 |
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| author | Z. M. Zagdyn A. A. Lutskiy A. A. Zhirkov O. A. Makarenko D. Ferrara R. Axelsson-Robertson I. Magalhaes N. A. Skrynnik V. N. Shabalin H. N. Dzhumaeva M. Maeurer S. V. Sidorenko Yu. V. Lobzin |
| author_facet | Z. M. Zagdyn A. A. Lutskiy A. A. Zhirkov O. A. Makarenko D. Ferrara R. Axelsson-Robertson I. Magalhaes N. A. Skrynnik V. N. Shabalin H. N. Dzhumaeva M. Maeurer S. V. Sidorenko Yu. V. Lobzin |
| author_sort | Z. M. Zagdyn |
| collection | DOAJ |
| description | Tuberculosis (TB) is one of the most significant problems in the Russian Health Care. Russia remains on the list of the 22 countries with a high TB incidence and on the third place in the world with a high prevalence of Drug Resistant TB [1]. It is urgently needed to develop new TB diagnostic methods as well as effective measures of the specific TB prevention, including a development of the novel vaccines, so we have to know better about the most immunogenic antigens of Mycobacterium Tuberculosis. We studied the Interferon-Q production in the whole blood after stimulating immune response with different proteins of Mycobacterium Tuberculosis in patients with active TB. The study results permitted us to evaluate the immunogenicity of the previously known proteins (Ag85a и ESAT-6) in comparison to the recently identified ones (Rv2957, Rv2958c и Rv0447), analyzing simultaneously their relation to tuberculin, as well as to antigens of the different viruses (Human Immunodeficiency Virus, Cytomegalovirus, Epstein-Barr Virus, Influenza Virus). Protein Rv2958c, unlike protein ESAT-6, showed the high immunogenicity in comparison to tuberculin. The expressed immunogenicity of protein Rv2958c might be indicated a possible greatest specificity of immune response to this antigen in TB patients. Meanwhile, bacillary tuberculosis was strongly associated with low immune response to this protein. Also we were found statistical differences in immune responses of patients to the different Mycobacterium Tuberculosis antigens depending on the drug sensitivity. In addition it was interesting to know about a significantly low immune response of patients with Drug Resistant TB to protein pp65 CMV. |
| first_indexed | 2024-03-12T03:13:07Z |
| format | Article |
| id | doaj.art-4e4e48e892344d4cbf59ba8f3524b2af |
| institution | Directory Open Access Journal |
| issn | 2072-6732 |
| language | Russian |
| last_indexed | 2024-03-12T03:13:07Z |
| publishDate | 2014-09-01 |
| publisher | Journal Infectology |
| record_format | Article |
| series | Журнал инфектологии |
| spelling | doaj.art-4e4e48e892344d4cbf59ba8f3524b2af2023-09-03T14:15:28ZrusJournal InfectologyЖурнал инфектологии2072-67322014-09-0152223110.22625/2072-6732-2013-5-2-22-31178Antigen induced production of υ-interferon ex vivo, in the peripheral blood of patients with active pulmonary tuberculosisZ. M. Zagdyn0A. A. Lutskiy1A. A. Zhirkov2O. A. Makarenko3D. Ferrara4R. Axelsson-Robertson5I. Magalhaes6N. A. Skrynnik7V. N. Shabalin8H. N. Dzhumaeva9M. Maeurer10S. V. Sidorenko11Yu. V. Lobzin12Санкт-Петербургский научно-исследовательский институт фтизиопульмонологии, Санкт-ПетербургНаучно-исследовательский институт детских инфекций ФМБА России, Санкт-ПетербургНаучно-исследовательский институт детских инфекций ФМБА России, Санкт-ПетербургНаучно-исследовательский институт детских инфекций ФМБА России, Санкт-ПетербургЦентр трансплантации аллогенных стволовых клеток, Каролинский университетский госпиталь, Стокгольм, Швеция Департамент микробиологии, опухолевой и клеточной биологии, Каролинский университет, Стокгольм, Швеция Университет Перуджии, Перуджия, Италия Отделение терапевтической иммунологии, департамента лабораторной медицины, Каролинский университет, Стокгольм, ШвецияЦентр трансплантации аллогенных стволовых клеток, Каролинский университетский госпиталь, Стокгольм, ШвецияДепартамент микробиологии, опухолевой и клеточной биологии, Каролинский университет, Стокгольм, ШвецияПротивотуберкулезный диспансер № 12, Санкт-ПетербургЗеленохолмская туберкулезная больница, Зеленый холм, Ленинградская областьЗеленохолмская туберкулезная больница, Зеленый холм, Ленинградская областьЦентр трансплантации аллогенных стволовых клеток, Каролинский университетский госпиталь, Стокгольм, Швеция Департамент микробиологии, опухолевой и клеточной биологии, Каролинский университет, Стокгольм, Швеция Отделение терапевтической иммунологии, департамента лабораторной медицины, Каролинский университет, Стокгольм, ШвецияНаучно-исследовательский институт детских инфекций ФМБА России, Санкт-ПетербургНаучно-исследовательский институт детских инфекций ФМБА России, Санкт-ПетербургTuberculosis (TB) is one of the most significant problems in the Russian Health Care. Russia remains on the list of the 22 countries with a high TB incidence and on the third place in the world with a high prevalence of Drug Resistant TB [1]. It is urgently needed to develop new TB diagnostic methods as well as effective measures of the specific TB prevention, including a development of the novel vaccines, so we have to know better about the most immunogenic antigens of Mycobacterium Tuberculosis. We studied the Interferon-Q production in the whole blood after stimulating immune response with different proteins of Mycobacterium Tuberculosis in patients with active TB. The study results permitted us to evaluate the immunogenicity of the previously known proteins (Ag85a и ESAT-6) in comparison to the recently identified ones (Rv2957, Rv2958c и Rv0447), analyzing simultaneously their relation to tuberculin, as well as to antigens of the different viruses (Human Immunodeficiency Virus, Cytomegalovirus, Epstein-Barr Virus, Influenza Virus). Protein Rv2958c, unlike protein ESAT-6, showed the high immunogenicity in comparison to tuberculin. The expressed immunogenicity of protein Rv2958c might be indicated a possible greatest specificity of immune response to this antigen in TB patients. Meanwhile, bacillary tuberculosis was strongly associated with low immune response to this protein. Also we were found statistical differences in immune responses of patients to the different Mycobacterium Tuberculosis antigens depending on the drug sensitivity. In addition it was interesting to know about a significantly low immune response of patients with Drug Resistant TB to protein pp65 CMV.https://journal.niidi.ru/jofin/article/view/164туберкулезлекарственно-устойчивый туберкулезпродукция интерферона гаммаспецифический иммунный ответтуберкулез и вирусные инфекции |
| spellingShingle | Z. M. Zagdyn A. A. Lutskiy A. A. Zhirkov O. A. Makarenko D. Ferrara R. Axelsson-Robertson I. Magalhaes N. A. Skrynnik V. N. Shabalin H. N. Dzhumaeva M. Maeurer S. V. Sidorenko Yu. V. Lobzin Antigen induced production of υ-interferon ex vivo, in the peripheral blood of patients with active pulmonary tuberculosis Журнал инфектологии туберкулез лекарственно-устойчивый туберкулез продукция интерферона гамма специфический иммунный ответ туберкулез и вирусные инфекции |
| title | Antigen induced production of υ-interferon ex vivo, in the peripheral blood of patients with active pulmonary tuberculosis |
| title_full | Antigen induced production of υ-interferon ex vivo, in the peripheral blood of patients with active pulmonary tuberculosis |
| title_fullStr | Antigen induced production of υ-interferon ex vivo, in the peripheral blood of patients with active pulmonary tuberculosis |
| title_full_unstemmed | Antigen induced production of υ-interferon ex vivo, in the peripheral blood of patients with active pulmonary tuberculosis |
| title_short | Antigen induced production of υ-interferon ex vivo, in the peripheral blood of patients with active pulmonary tuberculosis |
| title_sort | antigen induced production of υ interferon ex vivo in the peripheral blood of patients with active pulmonary tuberculosis |
| topic | туберкулез лекарственно-устойчивый туберкулез продукция интерферона гамма специфический иммунный ответ туберкулез и вирусные инфекции |
| url | https://journal.niidi.ru/jofin/article/view/164 |
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