Differentiation of Phytopathogenic Agrobacterium spp.
Due to the difficulties in differentiation of phytopathogenic Agrobacterium spp. and lack of a standardized protocol, we carried out selection and evaluation of suitable methods based on the bacterial physiological, genetic and pathogenic properties. Strains of Agrobacterium tumefaciens, A. rhizogen...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Institute of Pesticides and Environmental Protection
2011-01-01
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| Series: | Pesticidi i Fitomedicina |
| Subjects: | |
| Online Access: | http://www.pesting.org.rs/download.php/documents/26-3/26-3%20245-253.pdf |
| _version_ | 1819028160244613120 |
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| author | Nemanja Kuzmanović Milan Ivanović Anđelka Ćalić Katarina Gašić Aleksa Obradović |
| author_facet | Nemanja Kuzmanović Milan Ivanović Anđelka Ćalić Katarina Gašić Aleksa Obradović |
| author_sort | Nemanja Kuzmanović |
| collection | DOAJ |
| description | Due to the difficulties in differentiation of phytopathogenic Agrobacterium spp. and lack of a standardized protocol, we carried out selection and evaluation of suitable methods based on the bacterial physiological, genetic and pathogenic properties. Strains of Agrobacterium tumefaciens, A. rhizogenes and A. vitis were differentiated using standard bacteriological and molecular methods. The biochemical and physiological tests confirmed authenticity of the strains. Two duplex PCR methods were conducted with four different primer pairs. In all strains, presence of plasmid virD2 and virC pathogenicity genes was detected. Chromosomal pehA gene was determined in A. vitis strain. Pathogenicity was confirmed on carrot slices and young plants of tomato and sunflower. Strains of A. tumefaciens and A. vitis were pathogenic on all test plants, while strain of A. rhizogenes induced characteristic symptoms only on carrot slices. The tests used in this study provided reliable discrimination between the three species and confirmed their identity as tumorigenic (Ti)Agrobacterium tumefaciens and A. vitis, and rhizogenic (Ri) A. rhizogenes. |
| first_indexed | 2024-12-21T05:53:57Z |
| format | Article |
| id | doaj.art-4e4ef8f958544104816a03d9cdf628f5 |
| institution | Directory Open Access Journal |
| issn | 1820-3949 |
| language | English |
| last_indexed | 2024-12-21T05:53:57Z |
| publishDate | 2011-01-01 |
| publisher | Institute of Pesticides and Environmental Protection |
| record_format | Article |
| series | Pesticidi i Fitomedicina |
| spelling | doaj.art-4e4ef8f958544104816a03d9cdf628f52022-12-21T19:13:54ZengInstitute of Pesticides and Environmental ProtectionPesticidi i Fitomedicina1820-39492011-01-01263245253Differentiation of Phytopathogenic Agrobacterium spp.Nemanja KuzmanovićMilan IvanovićAnđelka ĆalićKatarina GašićAleksa ObradovićDue to the difficulties in differentiation of phytopathogenic Agrobacterium spp. and lack of a standardized protocol, we carried out selection and evaluation of suitable methods based on the bacterial physiological, genetic and pathogenic properties. Strains of Agrobacterium tumefaciens, A. rhizogenes and A. vitis were differentiated using standard bacteriological and molecular methods. The biochemical and physiological tests confirmed authenticity of the strains. Two duplex PCR methods were conducted with four different primer pairs. In all strains, presence of plasmid virD2 and virC pathogenicity genes was detected. Chromosomal pehA gene was determined in A. vitis strain. Pathogenicity was confirmed on carrot slices and young plants of tomato and sunflower. Strains of A. tumefaciens and A. vitis were pathogenic on all test plants, while strain of A. rhizogenes induced characteristic symptoms only on carrot slices. The tests used in this study provided reliable discrimination between the three species and confirmed their identity as tumorigenic (Ti)Agrobacterium tumefaciens and A. vitis, and rhizogenic (Ri) A. rhizogenes.http://www.pesting.org.rs/download.php/documents/26-3/26-3%20245-253.pdfCrown gallPlasmidAgrobacterium„Duplex“ PCRPathogenicity |
| spellingShingle | Nemanja Kuzmanović Milan Ivanović Anđelka Ćalić Katarina Gašić Aleksa Obradović Differentiation of Phytopathogenic Agrobacterium spp. Pesticidi i Fitomedicina Crown gall Plasmid Agrobacterium „Duplex“ PCR Pathogenicity |
| title | Differentiation of Phytopathogenic Agrobacterium spp. |
| title_full | Differentiation of Phytopathogenic Agrobacterium spp. |
| title_fullStr | Differentiation of Phytopathogenic Agrobacterium spp. |
| title_full_unstemmed | Differentiation of Phytopathogenic Agrobacterium spp. |
| title_short | Differentiation of Phytopathogenic Agrobacterium spp. |
| title_sort | differentiation of phytopathogenic agrobacterium spp |
| topic | Crown gall Plasmid Agrobacterium „Duplex“ PCR Pathogenicity |
| url | http://www.pesting.org.rs/download.php/documents/26-3/26-3%20245-253.pdf |
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