Discovery of a high-performance phage-derived promoter/repressor system for probiotic lactobacillus engineering
Abstract Background The Lactobacillaceae family comprises many species of great importance for the food and healthcare industries, with numerous strains identified as beneficial for humans and used as probiotics. Hence, there is a growing interest in engineering these probiotic bacteria as live biot...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-02-01
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| Series: | Microbial Cell Factories |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12934-024-02302-7 |
| _version_ | 1797272211942277120 |
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| author | Marc Blanch-Asensio Varun Sai Tadimarri Alina Wilk Shrikrishnan Sankaran |
| author_facet | Marc Blanch-Asensio Varun Sai Tadimarri Alina Wilk Shrikrishnan Sankaran |
| author_sort | Marc Blanch-Asensio |
| collection | DOAJ |
| description | Abstract Background The Lactobacillaceae family comprises many species of great importance for the food and healthcare industries, with numerous strains identified as beneficial for humans and used as probiotics. Hence, there is a growing interest in engineering these probiotic bacteria as live biotherapeutics for animals and humans. However, the genetic parts needed to regulate gene expression in these bacteria remain limited compared to model bacteria like E. coli or B. subtilis. To address this deficit, in this study, we selected and tested several bacteriophage-derived genetic parts with the potential to regulate transcription in lactobacilli. Results We screened genetic parts from 6 different lactobacilli-infecting phages and identified one promoter/repressor system with unprecedented functionality in Lactiplantibacillus plantarum WCFS1. The phage-derived promoter was found to achieve expression levels nearly 9-fold higher than the previously reported strongest promoter in this strain and the repressor was able to almost completely repress this expression by reducing it nearly 500-fold. Conclusions The new parts and insights gained from their engineering will enhance the genetic programmability of lactobacilli for healthcare and industrial applications. |
| first_indexed | 2024-03-07T14:25:08Z |
| format | Article |
| id | doaj.art-4e4f0bb172624f55b968c0b9534d0e69 |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-03-07T14:25:08Z |
| publishDate | 2024-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-4e4f0bb172624f55b968c0b9534d0e692024-03-06T08:06:27ZengBMCMicrobial Cell Factories1475-28592024-02-0123111310.1186/s12934-024-02302-7Discovery of a high-performance phage-derived promoter/repressor system for probiotic lactobacillus engineeringMarc Blanch-Asensio0Varun Sai Tadimarri1Alina Wilk2Shrikrishnan Sankaran3Bioprogrammable Materials, INM - Leibniz Institute for New MaterialsBioprogrammable Materials, INM - Leibniz Institute for New MaterialsBioprogrammable Materials, INM - Leibniz Institute for New MaterialsBioprogrammable Materials, INM - Leibniz Institute for New MaterialsAbstract Background The Lactobacillaceae family comprises many species of great importance for the food and healthcare industries, with numerous strains identified as beneficial for humans and used as probiotics. Hence, there is a growing interest in engineering these probiotic bacteria as live biotherapeutics for animals and humans. However, the genetic parts needed to regulate gene expression in these bacteria remain limited compared to model bacteria like E. coli or B. subtilis. To address this deficit, in this study, we selected and tested several bacteriophage-derived genetic parts with the potential to regulate transcription in lactobacilli. Results We screened genetic parts from 6 different lactobacilli-infecting phages and identified one promoter/repressor system with unprecedented functionality in Lactiplantibacillus plantarum WCFS1. The phage-derived promoter was found to achieve expression levels nearly 9-fold higher than the previously reported strongest promoter in this strain and the repressor was able to almost completely repress this expression by reducing it nearly 500-fold. Conclusions The new parts and insights gained from their engineering will enhance the genetic programmability of lactobacilli for healthcare and industrial applications.https://doi.org/10.1186/s12934-024-02302-7LactobacilliL. PlantarumProbiotic bacteriaBacteriophagePromoterRepressor. |
| spellingShingle | Marc Blanch-Asensio Varun Sai Tadimarri Alina Wilk Shrikrishnan Sankaran Discovery of a high-performance phage-derived promoter/repressor system for probiotic lactobacillus engineering Microbial Cell Factories Lactobacilli L. Plantarum Probiotic bacteria Bacteriophage Promoter Repressor. |
| title | Discovery of a high-performance phage-derived promoter/repressor system for probiotic lactobacillus engineering |
| title_full | Discovery of a high-performance phage-derived promoter/repressor system for probiotic lactobacillus engineering |
| title_fullStr | Discovery of a high-performance phage-derived promoter/repressor system for probiotic lactobacillus engineering |
| title_full_unstemmed | Discovery of a high-performance phage-derived promoter/repressor system for probiotic lactobacillus engineering |
| title_short | Discovery of a high-performance phage-derived promoter/repressor system for probiotic lactobacillus engineering |
| title_sort | discovery of a high performance phage derived promoter repressor system for probiotic lactobacillus engineering |
| topic | Lactobacilli L. Plantarum Probiotic bacteria Bacteriophage Promoter Repressor. |
| url | https://doi.org/10.1186/s12934-024-02302-7 |
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