Generation of an induced pluripotent stem cell JTUi005-A from a patient with neuronal intranuclear inclusion disease
Neuronal intranuclear inclusion disease is a neurodegenerative disease caused by expansion of GGC repeats in the 5′ untranslated region (5′ UTR) of NOTCH2NLC. An induced pluripotent stem cell (iPSC) line was generated from peripheral blood mononuclear cells of a 55-year-old male patient by expressin...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506122002872 |
| _version_ | 1811190524703408128 |
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| author | Yuwen Cao Wotu Tian Li Cao WenLu Lv Lan Zheng Xinghua Luan |
| author_facet | Yuwen Cao Wotu Tian Li Cao WenLu Lv Lan Zheng Xinghua Luan |
| author_sort | Yuwen Cao |
| collection | DOAJ |
| description | Neuronal intranuclear inclusion disease is a neurodegenerative disease caused by expansion of GGC repeats in the 5′ untranslated region (5′ UTR) of NOTCH2NLC. An induced pluripotent stem cell (iPSC) line was generated from peripheral blood mononuclear cells of a 55-year-old male patient by expressing a defined set of reprogramming factors (OCT4, SOX2, NANOG, LIN28, c-MYC and KLF4) carried on episomal vectors, and was validated for stem cell-like pluripotency, normal karyotype and capability of in vivo differentiation into three germ layers. The NIID-iPSC line serves as a promising tool for further research into pathogenic mechanism and potential therapeutic targets. |
| first_indexed | 2024-04-11T14:52:43Z |
| format | Article |
| id | doaj.art-4e4ff45434e24e11b5c2d75a64881211 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-04-11T14:52:43Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-4e4ff45434e24e11b5c2d75a648812112022-12-22T04:17:22ZengElsevierStem Cell Research1873-50612022-12-0165102938Generation of an induced pluripotent stem cell JTUi005-A from a patient with neuronal intranuclear inclusion diseaseYuwen Cao0Wotu Tian1Li Cao2WenLu Lv3Lan Zheng4Xinghua Luan5Department of Neurology, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai 200233, ChinaDepartment of Neurology, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai 200233, ChinaDepartment of Neurology, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai 200233, ChinaDepartment of Neurology, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai 200233, ChinaDepartment of Neurology, Minhang Hospital, Fudan University, Shanghai, China; Corresponding authors at: Department of Neurology, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai 200233, China (X. Luan).Department of Neurology, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai 200233, China; Department of Neurology, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; Corresponding authors at: Department of Neurology, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai 200233, China (X. Luan).Neuronal intranuclear inclusion disease is a neurodegenerative disease caused by expansion of GGC repeats in the 5′ untranslated region (5′ UTR) of NOTCH2NLC. An induced pluripotent stem cell (iPSC) line was generated from peripheral blood mononuclear cells of a 55-year-old male patient by expressing a defined set of reprogramming factors (OCT4, SOX2, NANOG, LIN28, c-MYC and KLF4) carried on episomal vectors, and was validated for stem cell-like pluripotency, normal karyotype and capability of in vivo differentiation into three germ layers. The NIID-iPSC line serves as a promising tool for further research into pathogenic mechanism and potential therapeutic targets.http://www.sciencedirect.com/science/article/pii/S1873506122002872 |
| spellingShingle | Yuwen Cao Wotu Tian Li Cao WenLu Lv Lan Zheng Xinghua Luan Generation of an induced pluripotent stem cell JTUi005-A from a patient with neuronal intranuclear inclusion disease Stem Cell Research |
| title | Generation of an induced pluripotent stem cell JTUi005-A from a patient with neuronal intranuclear inclusion disease |
| title_full | Generation of an induced pluripotent stem cell JTUi005-A from a patient with neuronal intranuclear inclusion disease |
| title_fullStr | Generation of an induced pluripotent stem cell JTUi005-A from a patient with neuronal intranuclear inclusion disease |
| title_full_unstemmed | Generation of an induced pluripotent stem cell JTUi005-A from a patient with neuronal intranuclear inclusion disease |
| title_short | Generation of an induced pluripotent stem cell JTUi005-A from a patient with neuronal intranuclear inclusion disease |
| title_sort | generation of an induced pluripotent stem cell jtui005 a from a patient with neuronal intranuclear inclusion disease |
| url | http://www.sciencedirect.com/science/article/pii/S1873506122002872 |
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