Foliar Application of Melatonin Positively Affects the Physio-Biochemical Characteristics of Cotton (<i>Gossypium hirsutum</i> L.) under the Combined Effects of Low Temperature and Salinity Stress
Low temperature and soil salinization during cotton sowing and seedling adversely affect cotton productivity. Exogenous melatonin (MT) can alleviate the damage caused to plants under non-biological stress; thus, applying MT is a means to improve the growth condition of crops under stress. However, a...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-10-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/12/21/3730 |
| _version_ | 1797631400802779136 |
|---|---|
| author | Yuanyuan Fu Lang Xin Abdoul Kader Mounkaila Hamani Weihao Sun Hongbo Wang Abubakar Sunusi Amin Xingpeng Wang Anzhen Qin Yang Gao |
| author_facet | Yuanyuan Fu Lang Xin Abdoul Kader Mounkaila Hamani Weihao Sun Hongbo Wang Abubakar Sunusi Amin Xingpeng Wang Anzhen Qin Yang Gao |
| author_sort | Yuanyuan Fu |
| collection | DOAJ |
| description | Low temperature and soil salinization during cotton sowing and seedling adversely affect cotton productivity. Exogenous melatonin (MT) can alleviate the damage caused to plants under non-biological stress; thus, applying MT is a means to improve the growth condition of crops under stress. However, achieving this goal requires a thorough understanding of the physiological regulatory mechanisms of MT on cotton seedlings under low temperature and salinity stress. This study could bring new knowledge on physio-biochemical mechanisms that improve the tolerance of cotton seedlings to combined effects of low temperature and salt stress using an exogenous foliar application of MT. The phytotron experiment comprised two temperature levels of cold stress and control and five MT treatments of 0, 50, 100, 150, and 200 μM and two salinity levels of 0 and 150 mM NaCl. Compared with the control treatments (non-salinity stress under cold stress and control), the combined stress of salt and low temperature reduced cotton seedlings’ biomass and net photosynthetic rate (<i>P<sub>n</sub></i>), aggravated the membrane damage, reduced the potassium (K<sup>+</sup>) content, and increased the sodium (Na<sup>+</sup>) accumulation in the leaves and roots. Under NaCl stress, exogenously sprayed 50–150 μM MT increased the biomass and gas exchange parameters of cotton seedlings under salt and low temperature combined with salt stress, reduced the degree of membrane damage, and regulated the antioxidant enzyme, ion homeostasis, transport, and absorption of cotton seedlings. The pairwise correlation analysis of each parameter using MT shows that the parameters with higher correlation with MT at cold stress are mainly malondialdehyde (MDA), peroxidase (POD), and catalase (CAT). The highest correlation coefficient at 25 °C is observed between the K<sup>+</sup> and Na<sup>+</sup> content in cotton seedlings. The conclusion indicates that under salt and low-temperature stress conditions, exogenous application of MT primarily regulates the levels of <i>P<sub>n</sub></i>, superoxide dismutase (SOD), andPOD in cotton seedlings, reduces Na<sup>+</sup> and MDA content, alleviates damage to cotton seedlings. Moreover, the most significant effect was observed when an exogenous application of 50–150 μM of MT was administered under these conditions. The current study’s findings could serve as a scientific foundation for salinity and low-temperature stress alleviation during the seedling stage of cotton growth. |
| first_indexed | 2024-03-11T11:23:19Z |
| format | Article |
| id | doaj.art-4e527f2b17a94f0abdc06b6577a39ac7 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T11:23:19Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-4e527f2b17a94f0abdc06b6577a39ac72023-11-10T15:10:20ZengMDPI AGPlants2223-77472023-10-011221373010.3390/plants12213730Foliar Application of Melatonin Positively Affects the Physio-Biochemical Characteristics of Cotton (<i>Gossypium hirsutum</i> L.) under the Combined Effects of Low Temperature and Salinity StressYuanyuan Fu0Lang Xin1Abdoul Kader Mounkaila Hamani2Weihao Sun3Hongbo Wang4Abubakar Sunusi Amin5Xingpeng Wang6Anzhen Qin7Yang Gao8College of Water Conservancy and Architecture Engineering, Tarim University, Alar 843300, ChinaCollege of Water Conservancy and Architecture Engineering, Tarim University, Alar 843300, ChinaCollege of Tropical Crops, Hainan University, Haikou 570100, China Key Laboratory of Crop Water Use and Regulation, Ministry of Agriculture and Rural Affairs, Institute of Farmland Irrigation, Chinese Academy of Agriculture Sciences, Xinxiang 453002, ChinaCollege of Water Conservancy and Architecture Engineering, Tarim University, Alar 843300, ChinaKey Laboratory of Crop Water Use and Regulation, Ministry of Agriculture and Rural Affairs, Institute of Farmland Irrigation, Chinese Academy of Agriculture Sciences, Xinxiang 453002, ChinaCollege of Water Conservancy and Architecture Engineering, Tarim University, Alar 843300, ChinaKey Laboratory of Crop Water Use and Regulation, Ministry of Agriculture and Rural Affairs, Institute of Farmland Irrigation, Chinese Academy of Agriculture Sciences, Xinxiang 453002, ChinaKey Laboratory of Crop Water Use and Regulation, Ministry of Agriculture and Rural Affairs, Institute of Farmland Irrigation, Chinese Academy of Agriculture Sciences, Xinxiang 453002, ChinaLow temperature and soil salinization during cotton sowing and seedling adversely affect cotton productivity. Exogenous melatonin (MT) can alleviate the damage caused to plants under non-biological stress; thus, applying MT is a means to improve the growth condition of crops under stress. However, achieving this goal requires a thorough understanding of the physiological regulatory mechanisms of MT on cotton seedlings under low temperature and salinity stress. This study could bring new knowledge on physio-biochemical mechanisms that improve the tolerance of cotton seedlings to combined effects of low temperature and salt stress using an exogenous foliar application of MT. The phytotron experiment comprised two temperature levels of cold stress and control and five MT treatments of 0, 50, 100, 150, and 200 μM and two salinity levels of 0 and 150 mM NaCl. Compared with the control treatments (non-salinity stress under cold stress and control), the combined stress of salt and low temperature reduced cotton seedlings’ biomass and net photosynthetic rate (<i>P<sub>n</sub></i>), aggravated the membrane damage, reduced the potassium (K<sup>+</sup>) content, and increased the sodium (Na<sup>+</sup>) accumulation in the leaves and roots. Under NaCl stress, exogenously sprayed 50–150 μM MT increased the biomass and gas exchange parameters of cotton seedlings under salt and low temperature combined with salt stress, reduced the degree of membrane damage, and regulated the antioxidant enzyme, ion homeostasis, transport, and absorption of cotton seedlings. The pairwise correlation analysis of each parameter using MT shows that the parameters with higher correlation with MT at cold stress are mainly malondialdehyde (MDA), peroxidase (POD), and catalase (CAT). The highest correlation coefficient at 25 °C is observed between the K<sup>+</sup> and Na<sup>+</sup> content in cotton seedlings. The conclusion indicates that under salt and low-temperature stress conditions, exogenous application of MT primarily regulates the levels of <i>P<sub>n</sub></i>, superoxide dismutase (SOD), andPOD in cotton seedlings, reduces Na<sup>+</sup> and MDA content, alleviates damage to cotton seedlings. Moreover, the most significant effect was observed when an exogenous application of 50–150 μM of MT was administered under these conditions. The current study’s findings could serve as a scientific foundation for salinity and low-temperature stress alleviation during the seedling stage of cotton growth.https://www.mdpi.com/2223-7747/12/21/3730cotton seedlingsion homeostasisfoliar melatoninmembrane damagelow temperaturesalt stress |
| spellingShingle | Yuanyuan Fu Lang Xin Abdoul Kader Mounkaila Hamani Weihao Sun Hongbo Wang Abubakar Sunusi Amin Xingpeng Wang Anzhen Qin Yang Gao Foliar Application of Melatonin Positively Affects the Physio-Biochemical Characteristics of Cotton (<i>Gossypium hirsutum</i> L.) under the Combined Effects of Low Temperature and Salinity Stress Plants cotton seedlings ion homeostasis foliar melatonin membrane damage low temperature salt stress |
| title | Foliar Application of Melatonin Positively Affects the Physio-Biochemical Characteristics of Cotton (<i>Gossypium hirsutum</i> L.) under the Combined Effects of Low Temperature and Salinity Stress |
| title_full | Foliar Application of Melatonin Positively Affects the Physio-Biochemical Characteristics of Cotton (<i>Gossypium hirsutum</i> L.) under the Combined Effects of Low Temperature and Salinity Stress |
| title_fullStr | Foliar Application of Melatonin Positively Affects the Physio-Biochemical Characteristics of Cotton (<i>Gossypium hirsutum</i> L.) under the Combined Effects of Low Temperature and Salinity Stress |
| title_full_unstemmed | Foliar Application of Melatonin Positively Affects the Physio-Biochemical Characteristics of Cotton (<i>Gossypium hirsutum</i> L.) under the Combined Effects of Low Temperature and Salinity Stress |
| title_short | Foliar Application of Melatonin Positively Affects the Physio-Biochemical Characteristics of Cotton (<i>Gossypium hirsutum</i> L.) under the Combined Effects of Low Temperature and Salinity Stress |
| title_sort | foliar application of melatonin positively affects the physio biochemical characteristics of cotton i gossypium hirsutum i l under the combined effects of low temperature and salinity stress |
| topic | cotton seedlings ion homeostasis foliar melatonin membrane damage low temperature salt stress |
| url | https://www.mdpi.com/2223-7747/12/21/3730 |
| work_keys_str_mv | AT yuanyuanfu foliarapplicationofmelatoninpositivelyaffectsthephysiobiochemicalcharacteristicsofcottonigossypiumhirsutumilunderthecombinedeffectsoflowtemperatureandsalinitystress AT langxin foliarapplicationofmelatoninpositivelyaffectsthephysiobiochemicalcharacteristicsofcottonigossypiumhirsutumilunderthecombinedeffectsoflowtemperatureandsalinitystress AT abdoulkadermounkailahamani foliarapplicationofmelatoninpositivelyaffectsthephysiobiochemicalcharacteristicsofcottonigossypiumhirsutumilunderthecombinedeffectsoflowtemperatureandsalinitystress AT weihaosun foliarapplicationofmelatoninpositivelyaffectsthephysiobiochemicalcharacteristicsofcottonigossypiumhirsutumilunderthecombinedeffectsoflowtemperatureandsalinitystress AT hongbowang foliarapplicationofmelatoninpositivelyaffectsthephysiobiochemicalcharacteristicsofcottonigossypiumhirsutumilunderthecombinedeffectsoflowtemperatureandsalinitystress AT abubakarsunusiamin foliarapplicationofmelatoninpositivelyaffectsthephysiobiochemicalcharacteristicsofcottonigossypiumhirsutumilunderthecombinedeffectsoflowtemperatureandsalinitystress AT xingpengwang foliarapplicationofmelatoninpositivelyaffectsthephysiobiochemicalcharacteristicsofcottonigossypiumhirsutumilunderthecombinedeffectsoflowtemperatureandsalinitystress AT anzhenqin foliarapplicationofmelatoninpositivelyaffectsthephysiobiochemicalcharacteristicsofcottonigossypiumhirsutumilunderthecombinedeffectsoflowtemperatureandsalinitystress AT yanggao foliarapplicationofmelatoninpositivelyaffectsthephysiobiochemicalcharacteristicsofcottonigossypiumhirsutumilunderthecombinedeffectsoflowtemperatureandsalinitystress |