Biological synthesis, characterization of three metal-based nanoparticles and their anticancer activities against hepatocellular carcinoma HepG2 cells
Treatment of liver cancer has always been a challenge for clinicians and development of appropriate drug against hepatocellular carcinoma is the major focus for researchers working in the field. The synthesis of metal-based nanoparticles (NPs) by green method for pharmacological uses has attained co...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-10-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651321006874 |
| _version_ | 1819086775062102016 |
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| author | Madiha Younas Muhammad Rizwan Muhammad Zubair Aqil Inam Shafaqat Ali |
| author_facet | Madiha Younas Muhammad Rizwan Muhammad Zubair Aqil Inam Shafaqat Ali |
| author_sort | Madiha Younas |
| collection | DOAJ |
| description | Treatment of liver cancer has always been a challenge for clinicians and development of appropriate drug against hepatocellular carcinoma is the major focus for researchers working in the field. The synthesis of metal-based nanoparticles (NPs) by green method for pharmacological uses has attained considerable attention recently. In current study three different NPs (AgO2, CeO2, CuO2) were synthesized by using Trianthima portulacastrum and Chinopodium quinoa leaf extracts. These biogenic NPs were analyzed by High-tech. approaches including Scanning Electron Microscopy (SEM) with Energy Dispersive X-ray (EDX) spectroscope, SEM-EDS spot analysis, elemental mapping and X-ray diffraction (XRD). The anticancer potential of these nanoparticles was estimated using MTT assay, against hepatic cancer cell line (HepG2). SEM secondary electron images presented the nano size of prepared particles in agglomerated form with few porous forms. Average size of Ag-, Ce-, and CuNPs was observed 19–24 nm, 8–12 nm, 13–15 nm respectively. Elemental mapping and EDS-spot analysis ratifies the formation of AgNPs, CeNPs, and CuNPs. These NPs have shown good anticancer activity at different concentrations against HepG2 cell line. Further studies are however needed to identify the molecular mechanisms of these anticancer activities. |
| first_indexed | 2024-12-21T21:25:36Z |
| format | Article |
| id | doaj.art-4e53014ad85645bf9bfc5e6c83c2658a |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-12-21T21:25:36Z |
| publishDate | 2021-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-4e53014ad85645bf9bfc5e6c83c2658a2022-12-21T18:49:46ZengElsevierEcotoxicology and Environmental Safety0147-65132021-10-01223112575Biological synthesis, characterization of three metal-based nanoparticles and their anticancer activities against hepatocellular carcinoma HepG2 cellsMadiha Younas0Muhammad Rizwan1Muhammad Zubair2Aqil Inam3Shafaqat Ali4Department of Bioinformatics and Biotechnology, Government College University Faisalabad, 38000 Faisalabad, PakistanDepartment of Environmental Sciences and Engineering, Government College University Faisalabad, 38000 Faisalabad, PakistanDepartment of Bioinformatics and Biotechnology, Government College University Faisalabad, 38000 Faisalabad, Pakistan; Corresponding author.Department of Metallurgy and Materials Engineering, University of Punjab, Lahore, PakistanDepartment of Environmental Sciences and Engineering, Government College University Faisalabad, 38000 Faisalabad, Pakistan; Department of Biological Sciences and Technology, China Medical University, Taichung 40402, Taiwan; Corresponding author at: Department of Environmental Sciences and Engineering, Government College University Faisalabad, 38000 Faisalabad, Pakistan.Treatment of liver cancer has always been a challenge for clinicians and development of appropriate drug against hepatocellular carcinoma is the major focus for researchers working in the field. The synthesis of metal-based nanoparticles (NPs) by green method for pharmacological uses has attained considerable attention recently. In current study three different NPs (AgO2, CeO2, CuO2) were synthesized by using Trianthima portulacastrum and Chinopodium quinoa leaf extracts. These biogenic NPs were analyzed by High-tech. approaches including Scanning Electron Microscopy (SEM) with Energy Dispersive X-ray (EDX) spectroscope, SEM-EDS spot analysis, elemental mapping and X-ray diffraction (XRD). The anticancer potential of these nanoparticles was estimated using MTT assay, against hepatic cancer cell line (HepG2). SEM secondary electron images presented the nano size of prepared particles in agglomerated form with few porous forms. Average size of Ag-, Ce-, and CuNPs was observed 19–24 nm, 8–12 nm, 13–15 nm respectively. Elemental mapping and EDS-spot analysis ratifies the formation of AgNPs, CeNPs, and CuNPs. These NPs have shown good anticancer activity at different concentrations against HepG2 cell line. Further studies are however needed to identify the molecular mechanisms of these anticancer activities.http://www.sciencedirect.com/science/article/pii/S0147651321006874Plant extractsNanoparticlesAnticancer activityCell line |
| spellingShingle | Madiha Younas Muhammad Rizwan Muhammad Zubair Aqil Inam Shafaqat Ali Biological synthesis, characterization of three metal-based nanoparticles and their anticancer activities against hepatocellular carcinoma HepG2 cells Ecotoxicology and Environmental Safety Plant extracts Nanoparticles Anticancer activity Cell line |
| title | Biological synthesis, characterization of three metal-based nanoparticles and their anticancer activities against hepatocellular carcinoma HepG2 cells |
| title_full | Biological synthesis, characterization of three metal-based nanoparticles and their anticancer activities against hepatocellular carcinoma HepG2 cells |
| title_fullStr | Biological synthesis, characterization of three metal-based nanoparticles and their anticancer activities against hepatocellular carcinoma HepG2 cells |
| title_full_unstemmed | Biological synthesis, characterization of three metal-based nanoparticles and their anticancer activities against hepatocellular carcinoma HepG2 cells |
| title_short | Biological synthesis, characterization of three metal-based nanoparticles and their anticancer activities against hepatocellular carcinoma HepG2 cells |
| title_sort | biological synthesis characterization of three metal based nanoparticles and their anticancer activities against hepatocellular carcinoma hepg2 cells |
| topic | Plant extracts Nanoparticles Anticancer activity Cell line |
| url | http://www.sciencedirect.com/science/article/pii/S0147651321006874 |
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