Orange Juice Attenuates Circulating miR-150-5p, miR-25-3p, and miR-451a in Healthy Smokers: A Randomized Crossover Study
Introduction: Tobacco smoke is associated with oxidative and inflammatory pathways, increasing the risk of chronic-degenerative diseases. Our goal was to evaluate the effects of acute “Pera” and “Moro” orange juice consumption on inflammatory processes and oxidative stress in microRNA (miRNA) expres...
| Main Authors: | , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-12-01
|
| Series: | Frontiers in Nutrition |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2021.775515/full |
| _version_ | 1818966239556403200 |
|---|---|
| author | Mariana S. Dorna Elizabete M. S. Barbosa Matheus A. Callegari Suzana E. Tanni Fernanda Chiuso-Minicucci Tainara F. Felix Ana L. Seneda Camila R. Correa Ana A. H. Fernandes Paula S. Azevedo Bertha F. Polegato Marcelo M. Rogero Sergio A. R. Paiva Leonardo A. M. Zornoff Patricia P. Reis Patricia P. Reis Marcos F. Minicucci |
| author_facet | Mariana S. Dorna Elizabete M. S. Barbosa Matheus A. Callegari Suzana E. Tanni Fernanda Chiuso-Minicucci Tainara F. Felix Ana L. Seneda Camila R. Correa Ana A. H. Fernandes Paula S. Azevedo Bertha F. Polegato Marcelo M. Rogero Sergio A. R. Paiva Leonardo A. M. Zornoff Patricia P. Reis Patricia P. Reis Marcos F. Minicucci |
| author_sort | Mariana S. Dorna |
| collection | DOAJ |
| description | Introduction: Tobacco smoke is associated with oxidative and inflammatory pathways, increasing the risk of chronic-degenerative diseases. Our goal was to evaluate the effects of acute “Pera” and “Moro” orange juice consumption on inflammatory processes and oxidative stress in microRNA (miRNA) expression in plasma from healthy smokers.Methods: This was a randomized crossover study that included healthy smokers over 18 years old. Blood samples were collected before and 11 h after beverage ingestion. Participants were instructed to drink 400 mL of Pera orange juice (Citrus sinensis), Moro orange juice (Citrus sinensis L. Osbeck), or water. Each subject drank the beverages in a 3-way crossover study design. Inflammatory and oxidative stress biomarkers and circulating miRNA expression profiles were determined. The subjects maintained their usual tobacco exposure during the experiment.Results: We included 18 individuals (12 men and 6 women), with 37.0 ± 12.0 years old. All subjects received the 3 interventions. Increased expression of circulating miRNAs (miR-150-5p, miR-25-3p, and miR-451a) was verified after cigarette smoking, which were attenuated after intake of both types of orange juice. There was no difference regarding serum levels of TNF-α, IL-6, MMP-9, and C-reactive protein. Despite the increased activity of serum superoxide dismutase and glutathione peroxidase after “Pera” or “Moro” orange juice intake, respectively, no changes in lipid hydroperoxide levels were detected.Conclusion: Tobaccos smokers showed increased expression of miR-150-5p, miR-25-3p, and miR-451a was noted, and attenuated by orange juice intake. miRNAs were predicted to regulate 244 target genes with roles in oxidative stress, PI3K-Akt, and MAPK signaling, which are pathways frequently involved in smoking-related cardiovascular diseases and cancer. |
| first_indexed | 2024-12-20T13:29:45Z |
| format | Article |
| id | doaj.art-4e568ee3555b4a34a4330d6a243195e6 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-12-20T13:29:45Z |
| publishDate | 2021-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-4e568ee3555b4a34a4330d6a243195e62022-12-21T19:39:09ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2021-12-01810.3389/fnut.2021.775515775515Orange Juice Attenuates Circulating miR-150-5p, miR-25-3p, and miR-451a in Healthy Smokers: A Randomized Crossover StudyMariana S. Dorna0Elizabete M. S. Barbosa1Matheus A. Callegari2Suzana E. Tanni3Fernanda Chiuso-Minicucci4Tainara F. Felix5Ana L. Seneda6Camila R. Correa7Ana A. H. Fernandes8Paula S. Azevedo9Bertha F. Polegato10Marcelo M. Rogero11Sergio A. R. Paiva12Leonardo A. M. Zornoff13Patricia P. Reis14Patricia P. Reis15Marcos F. Minicucci16Internal Medicine Department, Botucatu Medical School, São Paulo State University, UNESP, Botucatu, BrazilInternal Medicine Department, Botucatu Medical School, São Paulo State University, UNESP, Botucatu, BrazilInternal Medicine Department, Botucatu Medical School, São Paulo State University, UNESP, Botucatu, BrazilInternal Medicine Department, Botucatu Medical School, São Paulo State University, UNESP, Botucatu, BrazilInternal Medicine Department, Botucatu Medical School, São Paulo State University, UNESP, Botucatu, BrazilExperimental Research Unit, São Paulo State University, UNESP, Botucatu, BrazilExperimental Research Unit, São Paulo State University, UNESP, Botucatu, BrazilBotucatu Medical School, São Paulo State University, UNESP, Botucatu, BrazilChemistry and Biochemistry Department, Institute of Biosciences, São Paulo State University, UNESP, Botucatu, BrazilInternal Medicine Department, Botucatu Medical School, São Paulo State University, UNESP, Botucatu, BrazilInternal Medicine Department, Botucatu Medical School, São Paulo State University, UNESP, Botucatu, BrazilDepartment of Nutrition, School of Public Health, USP - University of São Paulo, São Paulo, BrazilInternal Medicine Department, Botucatu Medical School, São Paulo State University, UNESP, Botucatu, BrazilInternal Medicine Department, Botucatu Medical School, São Paulo State University, UNESP, Botucatu, BrazilExperimental Research Unit, São Paulo State University, UNESP, Botucatu, BrazilDepartment of Surgery and Orthopedics, São Paulo State University, UNESP, Botucatu, BrazilInternal Medicine Department, Botucatu Medical School, São Paulo State University, UNESP, Botucatu, BrazilIntroduction: Tobacco smoke is associated with oxidative and inflammatory pathways, increasing the risk of chronic-degenerative diseases. Our goal was to evaluate the effects of acute “Pera” and “Moro” orange juice consumption on inflammatory processes and oxidative stress in microRNA (miRNA) expression in plasma from healthy smokers.Methods: This was a randomized crossover study that included healthy smokers over 18 years old. Blood samples were collected before and 11 h after beverage ingestion. Participants were instructed to drink 400 mL of Pera orange juice (Citrus sinensis), Moro orange juice (Citrus sinensis L. Osbeck), or water. Each subject drank the beverages in a 3-way crossover study design. Inflammatory and oxidative stress biomarkers and circulating miRNA expression profiles were determined. The subjects maintained their usual tobacco exposure during the experiment.Results: We included 18 individuals (12 men and 6 women), with 37.0 ± 12.0 years old. All subjects received the 3 interventions. Increased expression of circulating miRNAs (miR-150-5p, miR-25-3p, and miR-451a) was verified after cigarette smoking, which were attenuated after intake of both types of orange juice. There was no difference regarding serum levels of TNF-α, IL-6, MMP-9, and C-reactive protein. Despite the increased activity of serum superoxide dismutase and glutathione peroxidase after “Pera” or “Moro” orange juice intake, respectively, no changes in lipid hydroperoxide levels were detected.Conclusion: Tobaccos smokers showed increased expression of miR-150-5p, miR-25-3p, and miR-451a was noted, and attenuated by orange juice intake. miRNAs were predicted to regulate 244 target genes with roles in oxidative stress, PI3K-Akt, and MAPK signaling, which are pathways frequently involved in smoking-related cardiovascular diseases and cancer.https://www.frontiersin.org/articles/10.3389/fnut.2021.775515/fullmicroRNAorange juicesmokersoxidative stressmolecular pathways |
| spellingShingle | Mariana S. Dorna Elizabete M. S. Barbosa Matheus A. Callegari Suzana E. Tanni Fernanda Chiuso-Minicucci Tainara F. Felix Ana L. Seneda Camila R. Correa Ana A. H. Fernandes Paula S. Azevedo Bertha F. Polegato Marcelo M. Rogero Sergio A. R. Paiva Leonardo A. M. Zornoff Patricia P. Reis Patricia P. Reis Marcos F. Minicucci Orange Juice Attenuates Circulating miR-150-5p, miR-25-3p, and miR-451a in Healthy Smokers: A Randomized Crossover Study Frontiers in Nutrition microRNA orange juice smokers oxidative stress molecular pathways |
| title | Orange Juice Attenuates Circulating miR-150-5p, miR-25-3p, and miR-451a in Healthy Smokers: A Randomized Crossover Study |
| title_full | Orange Juice Attenuates Circulating miR-150-5p, miR-25-3p, and miR-451a in Healthy Smokers: A Randomized Crossover Study |
| title_fullStr | Orange Juice Attenuates Circulating miR-150-5p, miR-25-3p, and miR-451a in Healthy Smokers: A Randomized Crossover Study |
| title_full_unstemmed | Orange Juice Attenuates Circulating miR-150-5p, miR-25-3p, and miR-451a in Healthy Smokers: A Randomized Crossover Study |
| title_short | Orange Juice Attenuates Circulating miR-150-5p, miR-25-3p, and miR-451a in Healthy Smokers: A Randomized Crossover Study |
| title_sort | orange juice attenuates circulating mir 150 5p mir 25 3p and mir 451a in healthy smokers a randomized crossover study |
| topic | microRNA orange juice smokers oxidative stress molecular pathways |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2021.775515/full |
| work_keys_str_mv | AT marianasdorna orangejuiceattenuatescirculatingmir1505pmir253pandmir451ainhealthysmokersarandomizedcrossoverstudy AT elizabetemsbarbosa orangejuiceattenuatescirculatingmir1505pmir253pandmir451ainhealthysmokersarandomizedcrossoverstudy AT matheusacallegari orangejuiceattenuatescirculatingmir1505pmir253pandmir451ainhealthysmokersarandomizedcrossoverstudy AT suzanaetanni orangejuiceattenuatescirculatingmir1505pmir253pandmir451ainhealthysmokersarandomizedcrossoverstudy AT fernandachiusominicucci orangejuiceattenuatescirculatingmir1505pmir253pandmir451ainhealthysmokersarandomizedcrossoverstudy AT tainaraffelix orangejuiceattenuatescirculatingmir1505pmir253pandmir451ainhealthysmokersarandomizedcrossoverstudy AT analseneda orangejuiceattenuatescirculatingmir1505pmir253pandmir451ainhealthysmokersarandomizedcrossoverstudy AT camilarcorrea orangejuiceattenuatescirculatingmir1505pmir253pandmir451ainhealthysmokersarandomizedcrossoverstudy AT anaahfernandes orangejuiceattenuatescirculatingmir1505pmir253pandmir451ainhealthysmokersarandomizedcrossoverstudy AT paulasazevedo orangejuiceattenuatescirculatingmir1505pmir253pandmir451ainhealthysmokersarandomizedcrossoverstudy AT berthafpolegato orangejuiceattenuatescirculatingmir1505pmir253pandmir451ainhealthysmokersarandomizedcrossoverstudy AT marcelomrogero orangejuiceattenuatescirculatingmir1505pmir253pandmir451ainhealthysmokersarandomizedcrossoverstudy AT sergioarpaiva orangejuiceattenuatescirculatingmir1505pmir253pandmir451ainhealthysmokersarandomizedcrossoverstudy AT leonardoamzornoff orangejuiceattenuatescirculatingmir1505pmir253pandmir451ainhealthysmokersarandomizedcrossoverstudy AT patriciapreis orangejuiceattenuatescirculatingmir1505pmir253pandmir451ainhealthysmokersarandomizedcrossoverstudy AT patriciapreis orangejuiceattenuatescirculatingmir1505pmir253pandmir451ainhealthysmokersarandomizedcrossoverstudy AT marcosfminicucci orangejuiceattenuatescirculatingmir1505pmir253pandmir451ainhealthysmokersarandomizedcrossoverstudy |