The effect of Arginine, Lysine and Histidine in the myosin secondary structure by circular dichroism and Raman spectroscopy
Amino acids containing L-Arginine (Arg), L-Lysine (Lys) and L-Histidine (His) are recognized to increase the solubility of myosin at low ionic strength, but there is a difference in the researchers’ views on their effects on the secondary structure of myosin. To elucidate which method is relatively...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-01-01
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| Series: | CyTA - Journal of Food |
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| Online Access: | http://dx.doi.org/10.1080/19476337.2019.1635648 |
| _version_ | 1818849962823254016 |
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| author | Tong Shi Li Yuan Jianlou Mu Ruichang Gao |
| author_facet | Tong Shi Li Yuan Jianlou Mu Ruichang Gao |
| author_sort | Tong Shi |
| collection | DOAJ |
| description | Amino acids containing L-Arginine (Arg), L-Lysine (Lys) and L-Histidine (His) are recognized to increase the solubility of myosin at low ionic strength, but there is a difference in the researchers’ views on their effects on the secondary structure of myosin. To elucidate which method is relatively appropriate for the determination of secondary structures of myosin added with Arg, Lys or His, Circular dichroism (CD) spectra and Raman spectroscopy were carried out respectively. Raman spectroscopy was more suitable than CD method for determination of the secondary structures of myosin added with His, while CD spectra was more accurate for the myosin samples added with Lys and the adequate concentration of Lys should be under 10 mM. Both CD and Raman methods could be applied to myosin added with Arg, however, the latter was recommended and the adequate additive content of Arg in CD method should be less than 10 mM. |
| first_indexed | 2024-12-19T06:41:34Z |
| format | Article |
| id | doaj.art-4e5704dfed874d9f9f8f992910ebb2e6 |
| institution | Directory Open Access Journal |
| issn | 1947-6337 1947-6345 |
| language | English |
| last_indexed | 2024-12-19T06:41:34Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | CyTA - Journal of Food |
| spelling | doaj.art-4e5704dfed874d9f9f8f992910ebb2e62022-12-21T20:32:03ZengTaylor & Francis GroupCyTA - Journal of Food1947-63371947-63452019-01-0117165666010.1080/19476337.2019.16356481635648The effect of Arginine, Lysine and Histidine in the myosin secondary structure by circular dichroism and Raman spectroscopyTong Shi0Li Yuan1Jianlou Mu2Ruichang Gao3Jiangsu UniversityJiangsu UniversityAgriculture University of Hebei ProvinceJiangsu UniversityAmino acids containing L-Arginine (Arg), L-Lysine (Lys) and L-Histidine (His) are recognized to increase the solubility of myosin at low ionic strength, but there is a difference in the researchers’ views on their effects on the secondary structure of myosin. To elucidate which method is relatively appropriate for the determination of secondary structures of myosin added with Arg, Lys or His, Circular dichroism (CD) spectra and Raman spectroscopy were carried out respectively. Raman spectroscopy was more suitable than CD method for determination of the secondary structures of myosin added with His, while CD spectra was more accurate for the myosin samples added with Lys and the adequate concentration of Lys should be under 10 mM. Both CD and Raman methods could be applied to myosin added with Arg, however, the latter was recommended and the adequate additive content of Arg in CD method should be less than 10 mM.http://dx.doi.org/10.1080/19476337.2019.1635648circular dichroismraman spectroscopymyosinsecondary structuresamino acids |
| spellingShingle | Tong Shi Li Yuan Jianlou Mu Ruichang Gao The effect of Arginine, Lysine and Histidine in the myosin secondary structure by circular dichroism and Raman spectroscopy CyTA - Journal of Food circular dichroism raman spectroscopy myosin secondary structures amino acids |
| title | The effect of Arginine, Lysine and Histidine in the myosin secondary structure by circular dichroism and Raman spectroscopy |
| title_full | The effect of Arginine, Lysine and Histidine in the myosin secondary structure by circular dichroism and Raman spectroscopy |
| title_fullStr | The effect of Arginine, Lysine and Histidine in the myosin secondary structure by circular dichroism and Raman spectroscopy |
| title_full_unstemmed | The effect of Arginine, Lysine and Histidine in the myosin secondary structure by circular dichroism and Raman spectroscopy |
| title_short | The effect of Arginine, Lysine and Histidine in the myosin secondary structure by circular dichroism and Raman spectroscopy |
| title_sort | effect of arginine lysine and histidine in the myosin secondary structure by circular dichroism and raman spectroscopy |
| topic | circular dichroism raman spectroscopy myosin secondary structures amino acids |
| url | http://dx.doi.org/10.1080/19476337.2019.1635648 |
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