Antiproliferative and apoptotic effects of pinocembrin in human prostate cancer cells
Pinocembrin, (5, 7-dihydroxyflavanone), has been shown to possess anticancer activity against various cancer cells. However, its effect against prostate cancer cells remained enigmatic. In this study, for the first time, we investigated whether pinocembrin could inhibit growth of human prostate canc...
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| Format: | Article |
| Language: | English |
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Bangladesh Pharmacological Society
2013-05-01
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| Series: | Bangladesh Journal of Pharmacology |
| Subjects: | |
| Online Access: | https://www.banglajol.info/index.php/BJP/article/view/14795 |
| _version_ | 1818236995526197248 |
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| author | Zhenyu Chen Azhar Rasul Chaoyue Zhao Faya Martin Millimouno Ichiro Tsuji Takaki Yamamura Rehana Iqbal Mahadev Malhi Xiaomeng Li Jiang Li |
| author_facet | Zhenyu Chen Azhar Rasul Chaoyue Zhao Faya Martin Millimouno Ichiro Tsuji Takaki Yamamura Rehana Iqbal Mahadev Malhi Xiaomeng Li Jiang Li |
| author_sort | Zhenyu Chen |
| collection | DOAJ |
| description | Pinocembrin, (5, 7-dihydroxyflavanone), has been shown to possess anticancer activity against various cancer cells. However, its effect against prostate cancer cells remained enigmatic. In this study, for the first time, we investigated whether pinocembrin could inhibit growth of human prostate cancer cells. MTT assay and flow cytometric analysis were performed to examine the effects of pinocembrin on cell proliferation, cell cycle, and apoptosis. The results revealed that pinocembrin attenuated the cell viability of both androgen-sensitive (LNCaP) as well as androgen-independent (PC3 and DU-145) prostate cancer cell lines, with different p53 status. Further characterization showed that pinocembrin markedly induced apoptosis of LNCaP cells and arrested cell cycle at S and G2/M phase and involved in the dissipation of mitochondrial membrane potential before culminating in apoptosis in pinocembrin-treated LNCaP cells. These in vitro results suggested that pinocembrin should be further examined for in vivo activity in human prostate cancer. |
| first_indexed | 2024-12-12T12:18:43Z |
| format | Article |
| id | doaj.art-4e575c519da0463b8c7e9224a1d3b2c7 |
| institution | Directory Open Access Journal |
| issn | 1991-0088 |
| language | English |
| last_indexed | 2024-12-12T12:18:43Z |
| publishDate | 2013-05-01 |
| publisher | Bangladesh Pharmacological Society |
| record_format | Article |
| series | Bangladesh Journal of Pharmacology |
| spelling | doaj.art-4e575c519da0463b8c7e9224a1d3b2c72022-12-22T00:24:43ZengBangladesh Pharmacological SocietyBangladesh Journal of Pharmacology1991-00882013-05-0183Antiproliferative and apoptotic effects of pinocembrin in human prostate cancer cellsZhenyu Chen0Azhar Rasul1Chaoyue Zhao2Faya Martin Millimouno3Ichiro Tsuji4Takaki Yamamura5Rehana Iqbal6Mahadev Malhi7Xiaomeng Li8Jiang Li9Dental Hospital, Jilin University, ChangchunThe Key Laboratory of Molecular Epigenetics of MOE, Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, Changchun 130024, China; Dental Hospital, Jilin University, Changchun 130041,Dental Hospital, Jilin University, ChangchunThe Key Laboratory of Molecular Epigenetics of MOE, Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, ChangchunDepartment of Public Health, Tohoku University, Sendai 980-8576Food and Nutrition, Morioka College, Iwate,Institute of Pure and Applied Biology, Bahauddin Zakariya University, Multan 60800,The Key Laboratory of Molecular Epigenetics of MOE, Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, Changchun 130024The Key Laboratory of Molecular Epigenetics of MOE, Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, Changchun 130024Dental Hospital, Jilin University, Changchun 130041Pinocembrin, (5, 7-dihydroxyflavanone), has been shown to possess anticancer activity against various cancer cells. However, its effect against prostate cancer cells remained enigmatic. In this study, for the first time, we investigated whether pinocembrin could inhibit growth of human prostate cancer cells. MTT assay and flow cytometric analysis were performed to examine the effects of pinocembrin on cell proliferation, cell cycle, and apoptosis. The results revealed that pinocembrin attenuated the cell viability of both androgen-sensitive (LNCaP) as well as androgen-independent (PC3 and DU-145) prostate cancer cell lines, with different p53 status. Further characterization showed that pinocembrin markedly induced apoptosis of LNCaP cells and arrested cell cycle at S and G2/M phase and involved in the dissipation of mitochondrial membrane potential before culminating in apoptosis in pinocembrin-treated LNCaP cells. These in vitro results suggested that pinocembrin should be further examined for in vivo activity in human prostate cancer.https://www.banglajol.info/index.php/BJP/article/view/14795ApoptosisLNCaP cellPinocembrinProstate cancer |
| spellingShingle | Zhenyu Chen Azhar Rasul Chaoyue Zhao Faya Martin Millimouno Ichiro Tsuji Takaki Yamamura Rehana Iqbal Mahadev Malhi Xiaomeng Li Jiang Li Antiproliferative and apoptotic effects of pinocembrin in human prostate cancer cells Bangladesh Journal of Pharmacology Apoptosis LNCaP cell Pinocembrin Prostate cancer |
| title | Antiproliferative and apoptotic effects of pinocembrin in human prostate cancer cells |
| title_full | Antiproliferative and apoptotic effects of pinocembrin in human prostate cancer cells |
| title_fullStr | Antiproliferative and apoptotic effects of pinocembrin in human prostate cancer cells |
| title_full_unstemmed | Antiproliferative and apoptotic effects of pinocembrin in human prostate cancer cells |
| title_short | Antiproliferative and apoptotic effects of pinocembrin in human prostate cancer cells |
| title_sort | antiproliferative and apoptotic effects of pinocembrin in human prostate cancer cells |
| topic | Apoptosis LNCaP cell Pinocembrin Prostate cancer |
| url | https://www.banglajol.info/index.php/BJP/article/view/14795 |
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