Registered report: Interactions between cancer stem cells and their niche govern metastatic colonization
The Reproducibility Project: Cancer Biology seeks to address growing concerns about reproducibility in scientific research by replicating selected results from a substantial number of high-profile papers in the field of cancer biology published between 2010 and 2012. This Registered report describes...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2015-06-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/06938 |
| _version_ | 1811253365341945856 |
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| author | Francesca Incardona M Mehdi Doroudchi Nawfal Ismail Alberto Carreno Erin Griner Minyoung Anna Lim Reproducibility Project: Cancer Biology |
| author_facet | Francesca Incardona M Mehdi Doroudchi Nawfal Ismail Alberto Carreno Erin Griner Minyoung Anna Lim Reproducibility Project: Cancer Biology |
| author_sort | Francesca Incardona |
| collection | DOAJ |
| description | The Reproducibility Project: Cancer Biology seeks to address growing concerns about reproducibility in scientific research by replicating selected results from a substantial number of high-profile papers in the field of cancer biology published between 2010 and 2012. This Registered report describes the proposed replication plan of key experiments from ‘Interactions between cancer stem cells and their niche govern metastatic colonization’ by Malanchi and colleagues, published in Nature in 2012 (Malanchi et al., 2012). The key experiments that will be replicated are those reported in Figures 2H, 3A, 3B, and S13. In these experiments, Malanchi and colleagues analyze messenger RNA levels of periostin (POSTN) in pulmonary fibroblasts, endothelial cells, and immune cells isolated from mice with micrometastases to determine which cell type is producing POSTN in the metastatic niche (Figure 2H; Malanchi et al., 2012). Additionally, they examine MMTV-PyMT control or POSTN null mice to test the effect of POSTN on primary tumor growth and metastasis (Figures 3A, 3B, and S13; Malanchi et al., 2012). The Reproducibility Project: Cancer Biology is a collaboration between the Center for Open Science and Science Exchange, and the results of the replications will be published in eLife. |
| first_indexed | 2024-04-12T16:49:50Z |
| format | Article |
| id | doaj.art-4e5ac63719204601bc3f90b849e21ec8 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T16:49:50Z |
| publishDate | 2015-06-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-4e5ac63719204601bc3f90b849e21ec82022-12-22T03:24:26ZengeLife Sciences Publications LtdeLife2050-084X2015-06-01410.7554/eLife.06938Registered report: Interactions between cancer stem cells and their niche govern metastatic colonizationFrancesca Incardona0M Mehdi Doroudchi1Nawfal Ismail2Alberto Carreno3Erin Griner4Minyoung Anna Lim5Reproducibility Project: Cancer BiologyBTS Research, San Diego, CaliforniaBTS Research, San Diego, CaliforniaBTS Research, San Diego, CaliforniaBTS Research, San Diego, CaliforniaUniversity of Virginia, Charlottesville, VirginiaEMD Millipore, Temecula, CaliforniaThe Reproducibility Project: Cancer Biology seeks to address growing concerns about reproducibility in scientific research by replicating selected results from a substantial number of high-profile papers in the field of cancer biology published between 2010 and 2012. This Registered report describes the proposed replication plan of key experiments from ‘Interactions between cancer stem cells and their niche govern metastatic colonization’ by Malanchi and colleagues, published in Nature in 2012 (Malanchi et al., 2012). The key experiments that will be replicated are those reported in Figures 2H, 3A, 3B, and S13. In these experiments, Malanchi and colleagues analyze messenger RNA levels of periostin (POSTN) in pulmonary fibroblasts, endothelial cells, and immune cells isolated from mice with micrometastases to determine which cell type is producing POSTN in the metastatic niche (Figure 2H; Malanchi et al., 2012). Additionally, they examine MMTV-PyMT control or POSTN null mice to test the effect of POSTN on primary tumor growth and metastasis (Figures 3A, 3B, and S13; Malanchi et al., 2012). The Reproducibility Project: Cancer Biology is a collaboration between the Center for Open Science and Science Exchange, and the results of the replications will be published in eLife.https://elifesciences.org/articles/06938Reproducibility Project: Cancer BiologymethodologyPostnbreast cancermetastasis |
| spellingShingle | Francesca Incardona M Mehdi Doroudchi Nawfal Ismail Alberto Carreno Erin Griner Minyoung Anna Lim Reproducibility Project: Cancer Biology Registered report: Interactions between cancer stem cells and their niche govern metastatic colonization eLife Reproducibility Project: Cancer Biology methodology Postn breast cancer metastasis |
| title | Registered report: Interactions between cancer stem cells and their niche govern metastatic colonization |
| title_full | Registered report: Interactions between cancer stem cells and their niche govern metastatic colonization |
| title_fullStr | Registered report: Interactions between cancer stem cells and their niche govern metastatic colonization |
| title_full_unstemmed | Registered report: Interactions between cancer stem cells and their niche govern metastatic colonization |
| title_short | Registered report: Interactions between cancer stem cells and their niche govern metastatic colonization |
| title_sort | registered report interactions between cancer stem cells and their niche govern metastatic colonization |
| topic | Reproducibility Project: Cancer Biology methodology Postn breast cancer metastasis |
| url | https://elifesciences.org/articles/06938 |
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