Third-Generation Cephalosporin Resistance in Intrinsic Colistin-Resistant Enterobacterales Isolated from Retail Meat
Consumption of retail meat contaminated with antimicrobial-resistant (AMR) bacteria is a common route for transmitting clinically relevant resistant bacteria to humans. Here, we investigated the genotypic and phenotypic resistance profiles of intrinsic colistin-resistant (ICR) Enterobacterales isola...
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MDPI AG
2021-11-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/10/12/1437 |
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| author | Justice Opare Odoi Sayo Takayanagi Montira Yossapol Michiyo Sugiyama Tetsuo Asai |
| author_facet | Justice Opare Odoi Sayo Takayanagi Montira Yossapol Michiyo Sugiyama Tetsuo Asai |
| author_sort | Justice Opare Odoi |
| collection | DOAJ |
| description | Consumption of retail meat contaminated with antimicrobial-resistant (AMR) bacteria is a common route for transmitting clinically relevant resistant bacteria to humans. Here, we investigated the genotypic and phenotypic resistance profiles of intrinsic colistin-resistant (ICR) Enterobacterales isolated from retail meats. ICR Enterobacterales were isolated from 103 samples of chicken, 103 samples of pork, and 104 samples of beef purchased from retail shops in Japan, using colistin-containing media, and their antimicrobial susceptibility was examined. <i>Serratia</i> spp. (440 isolates) showed resistance to cefotaxime (19 isolates, 4.3%), tetracycline (15 isolates, 3.4%), and other antimicrobials (<1%). <i>Hafnia</i> spp. (136) showed resistance to cefotaxime (12 isolates, 8.6%), ceftazidime (four isolates, 2.9%), and tetracycline (two isolates, 1.4%). <i>Proteus</i> spp. (39) showed resistance to chloramphenicol (four isolates, 10.3%), sulfamethoxazole-trimethoprim (four isolates, 10.3%), cefotaxime (two isolates, 5.1%), kanamycin (two isolates, 5.1%), and gentamicin (one isolate, 2.6%). <i>Cedecea</i> spp. (22) were resistant to tetracycline (two isolates, 9.1%) whereas <i>Morganella</i> spp. (11) were resistant to tetracycline (four isolates, 36.4%) and chloramphenicol (one isolate, 9.2%). The resistance genes <i>bla</i><sub>fonA</sub>, <i>bla</i><sub>ACC</sub>, and <i>bla</i><sub>DHA</sub> were detected in cefotaxime-resistant <i>Serratia</i> spp., <i>Hafnia</i> spp., and <i>Morganella</i> spp. isolates, respectively. This emergence of antimicrobial resistance in ICR Enterobacterales may pose a public health risk. |
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| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T04:39:28Z |
| publishDate | 2021-11-01 |
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| spelling | doaj.art-4e5b7795c97e4a64be82bcff0286e7832023-11-23T03:29:49ZengMDPI AGAntibiotics2079-63822021-11-011012143710.3390/antibiotics10121437Third-Generation Cephalosporin Resistance in Intrinsic Colistin-Resistant Enterobacterales Isolated from Retail MeatJustice Opare Odoi0Sayo Takayanagi1Montira Yossapol2Michiyo Sugiyama3Tetsuo Asai4Department of Applied Veterinary Science, United Graduate School of Veterinary Sciences, Gifu University, Gifu 501-1193, JapanFaculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, JapanBioveterinary Research Unit, Faculty of Veterinary Sciences, Mahasarakham University, Maha Sarakham 44000, ThailandDepartment of Applied Veterinary Science, United Graduate School of Veterinary Sciences, Gifu University, Gifu 501-1193, JapanDepartment of Applied Veterinary Science, United Graduate School of Veterinary Sciences, Gifu University, Gifu 501-1193, JapanConsumption of retail meat contaminated with antimicrobial-resistant (AMR) bacteria is a common route for transmitting clinically relevant resistant bacteria to humans. Here, we investigated the genotypic and phenotypic resistance profiles of intrinsic colistin-resistant (ICR) Enterobacterales isolated from retail meats. ICR Enterobacterales were isolated from 103 samples of chicken, 103 samples of pork, and 104 samples of beef purchased from retail shops in Japan, using colistin-containing media, and their antimicrobial susceptibility was examined. <i>Serratia</i> spp. (440 isolates) showed resistance to cefotaxime (19 isolates, 4.3%), tetracycline (15 isolates, 3.4%), and other antimicrobials (<1%). <i>Hafnia</i> spp. (136) showed resistance to cefotaxime (12 isolates, 8.6%), ceftazidime (four isolates, 2.9%), and tetracycline (two isolates, 1.4%). <i>Proteus</i> spp. (39) showed resistance to chloramphenicol (four isolates, 10.3%), sulfamethoxazole-trimethoprim (four isolates, 10.3%), cefotaxime (two isolates, 5.1%), kanamycin (two isolates, 5.1%), and gentamicin (one isolate, 2.6%). <i>Cedecea</i> spp. (22) were resistant to tetracycline (two isolates, 9.1%) whereas <i>Morganella</i> spp. (11) were resistant to tetracycline (four isolates, 36.4%) and chloramphenicol (one isolate, 9.2%). The resistance genes <i>bla</i><sub>fonA</sub>, <i>bla</i><sub>ACC</sub>, and <i>bla</i><sub>DHA</sub> were detected in cefotaxime-resistant <i>Serratia</i> spp., <i>Hafnia</i> spp., and <i>Morganella</i> spp. isolates, respectively. This emergence of antimicrobial resistance in ICR Enterobacterales may pose a public health risk.https://www.mdpi.com/2079-6382/10/12/1437Enterobacteralesintrinsic colistin resistanceretail meat |
| spellingShingle | Justice Opare Odoi Sayo Takayanagi Montira Yossapol Michiyo Sugiyama Tetsuo Asai Third-Generation Cephalosporin Resistance in Intrinsic Colistin-Resistant Enterobacterales Isolated from Retail Meat Antibiotics Enterobacterales intrinsic colistin resistance retail meat |
| title | Third-Generation Cephalosporin Resistance in Intrinsic Colistin-Resistant Enterobacterales Isolated from Retail Meat |
| title_full | Third-Generation Cephalosporin Resistance in Intrinsic Colistin-Resistant Enterobacterales Isolated from Retail Meat |
| title_fullStr | Third-Generation Cephalosporin Resistance in Intrinsic Colistin-Resistant Enterobacterales Isolated from Retail Meat |
| title_full_unstemmed | Third-Generation Cephalosporin Resistance in Intrinsic Colistin-Resistant Enterobacterales Isolated from Retail Meat |
| title_short | Third-Generation Cephalosporin Resistance in Intrinsic Colistin-Resistant Enterobacterales Isolated from Retail Meat |
| title_sort | third generation cephalosporin resistance in intrinsic colistin resistant enterobacterales isolated from retail meat |
| topic | Enterobacterales intrinsic colistin resistance retail meat |
| url | https://www.mdpi.com/2079-6382/10/12/1437 |
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