The Role of <i>Allium subhirsutum</i> L. in the Attenuation of Dermal Wounds by Modulating Oxidative Stress and Inflammation in <i>Wistar</i> Albino Rats
In our study, <i>Allium subhirsutum</i> L. (AS) was investigated to assess its phenolic profile and bioactive molecules including flavonoids and organosulfur compounds. The antioxidant potential of AS and wound healing activity were addressed using skin wound healing and oxidative stress...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-08-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/26/16/4875 |
| _version_ | 1827684569994756096 |
|---|---|
| author | Mongi Saoudi Riadh Badraoui Ahlem Chira Mohd Saeed Nouha Bouali Salem Elkahoui Jahoor M. Alam Choumous Kallel Abdelfattah El Feki |
| author_facet | Mongi Saoudi Riadh Badraoui Ahlem Chira Mohd Saeed Nouha Bouali Salem Elkahoui Jahoor M. Alam Choumous Kallel Abdelfattah El Feki |
| author_sort | Mongi Saoudi |
| collection | DOAJ |
| description | In our study, <i>Allium subhirsutum</i> L. (AS) was investigated to assess its phenolic profile and bioactive molecules including flavonoids and organosulfur compounds. The antioxidant potential of AS and wound healing activity were addressed using skin wound healing and oxidative stress and inflammation marker estimation in rat models. Phytochemical and antiradical activities of AS extract (ASE) and oil (ASO) were studied. The rats were randomly assigned to four groups: group I served as a control and was treated with simple ointment base, group II was treated with ASE ointment, group III was treated with ASO ointment and group IV (reference group; Ref) was treated with a reference drug “Cytolcentella<sup>®</sup> cream”. Phytochemical screening showed that total phenols (215 ± 3.5 mg GAE/g) and flavonoids (172.4 ± 3.1 mg QE/g) were higher in the ASO than the ASE group. The results of the antioxidant properties showed that ASO exhibited the highest DPPH free radical scavenging potential (IC50 = 0.136 ± 0.07 mg/mL), FRAP test (IC50 = 0.013 ± 0.006 mg/mL), ABTS test (IC50 = 0.52 ± 0.03 mg/mL) and total antioxidant capacity (IC50 = 0.34 ± 0.06 mg/mL). In the wound healing study, topical application of ASO performed the fastest wound-repairing process estimated by a chromatic study, percentage wound closure, fibrinogen level and oxidative damage status, as compared to ASE, the Cytolcentella reference drug and the untreated rats. The use of AS extract and oil were also associated with the attenuation of oxidative stress damage in the wound-healing treated rats. Overall, the results provided that AS, particularly ASO, has a potential medicinal value to act as effective skin wound healing agent. |
| first_indexed | 2024-03-10T08:33:05Z |
| format | Article |
| id | doaj.art-4e5ba913d8bc4a76b9cf72e991cfdd9f |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T08:33:05Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-4e5ba913d8bc4a76b9cf72e991cfdd9f2023-11-22T08:53:16ZengMDPI AGMolecules1420-30492021-08-012616487510.3390/molecules26164875The Role of <i>Allium subhirsutum</i> L. in the Attenuation of Dermal Wounds by Modulating Oxidative Stress and Inflammation in <i>Wistar</i> Albino RatsMongi Saoudi0Riadh Badraoui1Ahlem Chira2Mohd Saeed3Nouha Bouali4Salem Elkahoui5Jahoor M. Alam6Choumous Kallel7Abdelfattah El Feki8Animal Ecophysiology Laboratory, Sciences Faculty of Sfax, University of Sfax, Sfax 3054, TunisiaLaboratory of General Biology, Department of Biology, University of Ha’il, Ha’il 81451, Saudi ArabiaAnimal Ecophysiology Laboratory, Sciences Faculty of Sfax, University of Sfax, Sfax 3054, TunisiaLaboratory of General Biology, Department of Biology, University of Ha’il, Ha’il 81451, Saudi ArabiaLaboratory of General Biology, Department of Biology, University of Ha’il, Ha’il 81451, Saudi ArabiaLaboratory of General Biology, Department of Biology, University of Ha’il, Ha’il 81451, Saudi ArabiaLaboratory of General Biology, Department of Biology, University of Ha’il, Ha’il 81451, Saudi ArabiaHematology Laboratory, Hospital Habib Bourguiba, Sfax 3029, TunisiaAnimal Ecophysiology Laboratory, Sciences Faculty of Sfax, University of Sfax, Sfax 3054, TunisiaIn our study, <i>Allium subhirsutum</i> L. (AS) was investigated to assess its phenolic profile and bioactive molecules including flavonoids and organosulfur compounds. The antioxidant potential of AS and wound healing activity were addressed using skin wound healing and oxidative stress and inflammation marker estimation in rat models. Phytochemical and antiradical activities of AS extract (ASE) and oil (ASO) were studied. The rats were randomly assigned to four groups: group I served as a control and was treated with simple ointment base, group II was treated with ASE ointment, group III was treated with ASO ointment and group IV (reference group; Ref) was treated with a reference drug “Cytolcentella<sup>®</sup> cream”. Phytochemical screening showed that total phenols (215 ± 3.5 mg GAE/g) and flavonoids (172.4 ± 3.1 mg QE/g) were higher in the ASO than the ASE group. The results of the antioxidant properties showed that ASO exhibited the highest DPPH free radical scavenging potential (IC50 = 0.136 ± 0.07 mg/mL), FRAP test (IC50 = 0.013 ± 0.006 mg/mL), ABTS test (IC50 = 0.52 ± 0.03 mg/mL) and total antioxidant capacity (IC50 = 0.34 ± 0.06 mg/mL). In the wound healing study, topical application of ASO performed the fastest wound-repairing process estimated by a chromatic study, percentage wound closure, fibrinogen level and oxidative damage status, as compared to ASE, the Cytolcentella reference drug and the untreated rats. The use of AS extract and oil were also associated with the attenuation of oxidative stress damage in the wound-healing treated rats. Overall, the results provided that AS, particularly ASO, has a potential medicinal value to act as effective skin wound healing agent.https://www.mdpi.com/1420-3049/26/16/4875<i>Allium subhirsutum</i> L.wound-healing activityantioxidant potentialinflammatory markeroxidative stress |
| spellingShingle | Mongi Saoudi Riadh Badraoui Ahlem Chira Mohd Saeed Nouha Bouali Salem Elkahoui Jahoor M. Alam Choumous Kallel Abdelfattah El Feki The Role of <i>Allium subhirsutum</i> L. in the Attenuation of Dermal Wounds by Modulating Oxidative Stress and Inflammation in <i>Wistar</i> Albino Rats Molecules <i>Allium subhirsutum</i> L. wound-healing activity antioxidant potential inflammatory marker oxidative stress |
| title | The Role of <i>Allium subhirsutum</i> L. in the Attenuation of Dermal Wounds by Modulating Oxidative Stress and Inflammation in <i>Wistar</i> Albino Rats |
| title_full | The Role of <i>Allium subhirsutum</i> L. in the Attenuation of Dermal Wounds by Modulating Oxidative Stress and Inflammation in <i>Wistar</i> Albino Rats |
| title_fullStr | The Role of <i>Allium subhirsutum</i> L. in the Attenuation of Dermal Wounds by Modulating Oxidative Stress and Inflammation in <i>Wistar</i> Albino Rats |
| title_full_unstemmed | The Role of <i>Allium subhirsutum</i> L. in the Attenuation of Dermal Wounds by Modulating Oxidative Stress and Inflammation in <i>Wistar</i> Albino Rats |
| title_short | The Role of <i>Allium subhirsutum</i> L. in the Attenuation of Dermal Wounds by Modulating Oxidative Stress and Inflammation in <i>Wistar</i> Albino Rats |
| title_sort | role of i allium subhirsutum i l in the attenuation of dermal wounds by modulating oxidative stress and inflammation in i wistar i albino rats |
| topic | <i>Allium subhirsutum</i> L. wound-healing activity antioxidant potential inflammatory marker oxidative stress |
| url | https://www.mdpi.com/1420-3049/26/16/4875 |
| work_keys_str_mv | AT mongisaoudi theroleofialliumsubhirsutumilintheattenuationofdermalwoundsbymodulatingoxidativestressandinflammationiniwistarialbinorats AT riadhbadraoui theroleofialliumsubhirsutumilintheattenuationofdermalwoundsbymodulatingoxidativestressandinflammationiniwistarialbinorats AT ahlemchira theroleofialliumsubhirsutumilintheattenuationofdermalwoundsbymodulatingoxidativestressandinflammationiniwistarialbinorats AT mohdsaeed theroleofialliumsubhirsutumilintheattenuationofdermalwoundsbymodulatingoxidativestressandinflammationiniwistarialbinorats AT nouhabouali theroleofialliumsubhirsutumilintheattenuationofdermalwoundsbymodulatingoxidativestressandinflammationiniwistarialbinorats AT salemelkahoui theroleofialliumsubhirsutumilintheattenuationofdermalwoundsbymodulatingoxidativestressandinflammationiniwistarialbinorats AT jahoormalam theroleofialliumsubhirsutumilintheattenuationofdermalwoundsbymodulatingoxidativestressandinflammationiniwistarialbinorats AT choumouskallel theroleofialliumsubhirsutumilintheattenuationofdermalwoundsbymodulatingoxidativestressandinflammationiniwistarialbinorats AT abdelfattahelfeki theroleofialliumsubhirsutumilintheattenuationofdermalwoundsbymodulatingoxidativestressandinflammationiniwistarialbinorats AT mongisaoudi roleofialliumsubhirsutumilintheattenuationofdermalwoundsbymodulatingoxidativestressandinflammationiniwistarialbinorats AT riadhbadraoui roleofialliumsubhirsutumilintheattenuationofdermalwoundsbymodulatingoxidativestressandinflammationiniwistarialbinorats AT ahlemchira roleofialliumsubhirsutumilintheattenuationofdermalwoundsbymodulatingoxidativestressandinflammationiniwistarialbinorats AT mohdsaeed roleofialliumsubhirsutumilintheattenuationofdermalwoundsbymodulatingoxidativestressandinflammationiniwistarialbinorats AT nouhabouali roleofialliumsubhirsutumilintheattenuationofdermalwoundsbymodulatingoxidativestressandinflammationiniwistarialbinorats AT salemelkahoui roleofialliumsubhirsutumilintheattenuationofdermalwoundsbymodulatingoxidativestressandinflammationiniwistarialbinorats AT jahoormalam roleofialliumsubhirsutumilintheattenuationofdermalwoundsbymodulatingoxidativestressandinflammationiniwistarialbinorats AT choumouskallel roleofialliumsubhirsutumilintheattenuationofdermalwoundsbymodulatingoxidativestressandinflammationiniwistarialbinorats AT abdelfattahelfeki roleofialliumsubhirsutumilintheattenuationofdermalwoundsbymodulatingoxidativestressandinflammationiniwistarialbinorats |