Comparative transcriptomic analysis of Lactiplantibacillus plantarum RS66CD biofilm in high-salt conditions and planktonic cells
Background Lactiplantibacillus plantarum (L. plantarum), a dominant strain in traditional fermented foods, is widely used in fermentation industry because of its fast acid production. However, L. plantarum is easily inactivated due to acidity, high temperature and other factors. The formation of bio...
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PeerJ Inc.
2020-08-01
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| Online Access: | https://peerj.com/articles/9639.pdf |
| _version_ | 1797419694532067328 |
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| author | Xiaolin Ao Jiawei Zhao Junling Yan Shuliang Liu Ke Zhao |
| author_facet | Xiaolin Ao Jiawei Zhao Junling Yan Shuliang Liu Ke Zhao |
| author_sort | Xiaolin Ao |
| collection | DOAJ |
| description | Background Lactiplantibacillus plantarum (L. plantarum), a dominant strain in traditional fermented foods, is widely used in fermentation industry because of its fast acid production. However, L. plantarum is easily inactivated due to acidity, high temperature and other factors. The formation of biofilm by bacteria can effectively increase environmental tolerance. Therefore, it is important to improve the environmental tolerance of L. plantarum by studying its biofilm formation conditions and regulatory mechanisms. Methods After determining a suitable NaCl concentration for promoting biofilm formation, L. plantarum was grown with 48 g L−1 NaCl. Differential gene expressions in L. plantarum biofilm vs. planktonic cells were analyzed using RNA sequencing and validated using qPCR. Result L. plantarum RS66CD biofilm formation formed highest amount of when grown at 48 g L−1 NaCl. Altogether 447 genes were up-regulated and 426 genes were down-regulated in the biofilm. KEGG pathway analysis showed that genes coding for D-Alanine metabolism, peptidoglycan biosynthesis, two-component system, carbon metabolism, bacterial secretion system, lysine biosynthesis and fatty acid metabolism were crucial for biofilm formation. In addition, eight other genes related to biofilm formation were differentially expressed. Our results provide insights into the differential gene expression involved in biofilm formation, which can help to reveal gene regulation during L. plantarum biofilm formation. |
| first_indexed | 2024-03-09T06:52:00Z |
| format | Article |
| id | doaj.art-4e5bdf627d114160b88a2b83bebcfe72 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:52:00Z |
| publishDate | 2020-08-01 |
| publisher | PeerJ Inc. |
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| spelling | doaj.art-4e5bdf627d114160b88a2b83bebcfe722023-12-03T10:25:10ZengPeerJ Inc.PeerJ2167-83592020-08-018e963910.7717/peerj.9639Comparative transcriptomic analysis of Lactiplantibacillus plantarum RS66CD biofilm in high-salt conditions and planktonic cellsXiaolin Ao0Jiawei Zhao1Junling Yan2Shuliang Liu3Ke Zhao4College of Food Science, Sichuan Agricultural University, Ya’an, Sichuan, ChinaCollege of Food Science, Sichuan Agricultural University, Ya’an, Sichuan, ChinaCollege of Food Science, Sichuan Agricultural University, Ya’an, Sichuan, ChinaCollege of Food Science, Sichuan Agricultural University, Ya’an, Sichuan, ChinaColloge of Resources, Sichuan Agricultural University, Cheng‘du’, ChinaBackground Lactiplantibacillus plantarum (L. plantarum), a dominant strain in traditional fermented foods, is widely used in fermentation industry because of its fast acid production. However, L. plantarum is easily inactivated due to acidity, high temperature and other factors. The formation of biofilm by bacteria can effectively increase environmental tolerance. Therefore, it is important to improve the environmental tolerance of L. plantarum by studying its biofilm formation conditions and regulatory mechanisms. Methods After determining a suitable NaCl concentration for promoting biofilm formation, L. plantarum was grown with 48 g L−1 NaCl. Differential gene expressions in L. plantarum biofilm vs. planktonic cells were analyzed using RNA sequencing and validated using qPCR. Result L. plantarum RS66CD biofilm formation formed highest amount of when grown at 48 g L−1 NaCl. Altogether 447 genes were up-regulated and 426 genes were down-regulated in the biofilm. KEGG pathway analysis showed that genes coding for D-Alanine metabolism, peptidoglycan biosynthesis, two-component system, carbon metabolism, bacterial secretion system, lysine biosynthesis and fatty acid metabolism were crucial for biofilm formation. In addition, eight other genes related to biofilm formation were differentially expressed. Our results provide insights into the differential gene expression involved in biofilm formation, which can help to reveal gene regulation during L. plantarum biofilm formation.https://peerj.com/articles/9639.pdfBiofilmTranscriptomicsGene expressionLactiplantibacillus plantarum |
| spellingShingle | Xiaolin Ao Jiawei Zhao Junling Yan Shuliang Liu Ke Zhao Comparative transcriptomic analysis of Lactiplantibacillus plantarum RS66CD biofilm in high-salt conditions and planktonic cells PeerJ Biofilm Transcriptomics Gene expression Lactiplantibacillus plantarum |
| title | Comparative transcriptomic analysis of Lactiplantibacillus plantarum RS66CD biofilm in high-salt conditions and planktonic cells |
| title_full | Comparative transcriptomic analysis of Lactiplantibacillus plantarum RS66CD biofilm in high-salt conditions and planktonic cells |
| title_fullStr | Comparative transcriptomic analysis of Lactiplantibacillus plantarum RS66CD biofilm in high-salt conditions and planktonic cells |
| title_full_unstemmed | Comparative transcriptomic analysis of Lactiplantibacillus plantarum RS66CD biofilm in high-salt conditions and planktonic cells |
| title_short | Comparative transcriptomic analysis of Lactiplantibacillus plantarum RS66CD biofilm in high-salt conditions and planktonic cells |
| title_sort | comparative transcriptomic analysis of lactiplantibacillus plantarum rs66cd biofilm in high salt conditions and planktonic cells |
| topic | Biofilm Transcriptomics Gene expression Lactiplantibacillus plantarum |
| url | https://peerj.com/articles/9639.pdf |
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