Influence of Pine Bark Tannin on Bacterial Pathogens Growth and Nitrogen Compounds on Changes in Composted Poultry Litter
ABSTRACT To study the antimicrobial and uric acid-preserving activity of pine bark tannin on poultry litter composting, antibiotic-free wood chip-based poultry litter was distributed (11 g) to 50-mL conical centrifuge tubes and immediately amended with 1.3 mL0.4 M sodium phosphate buffer (control) o...
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| Format: | Article |
| Language: | English |
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Fundação APINCO de Ciência e Tecnologia Avícolas
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| Series: | Brazilian Journal of Poultry Science |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000100305&lng=en&tlng=en |
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| author | C Arzola-Alavarez Y Castillo-Castillo RC Anderson ME Hume O Ruiz-Barrera BR Min A Arzola-Rubio RC Beier J Salinas-Chavira |
| author_facet | C Arzola-Alavarez Y Castillo-Castillo RC Anderson ME Hume O Ruiz-Barrera BR Min A Arzola-Rubio RC Beier J Salinas-Chavira |
| author_sort | C Arzola-Alavarez |
| collection | DOAJ |
| description | ABSTRACT To study the antimicrobial and uric acid-preserving activity of pine bark tannin on poultry litter composting, antibiotic-free wood chip-based poultry litter was distributed (11 g) to 50-mL conical centrifuge tubes and immediately amended with 1.3 mL0.4 M sodium phosphate buffer (control) or with 1.3 mL condensed tannins from pine bark (Pinus palustris; 9 % tannin wt/vol in water). All tubes (n = 3 tubes/treatment) were inoculated with a novobiocin and naladixic acid-resistant Salmo-nella typhimurium (STNN) to achieve 3.0 log10 CFU/g and incubated at 37oC for 3 days to simulate an initial compost period. Wildtype E. coli and the challenge STNN strain as well as concentrations of ammonia, uric acid and urea were measured on days 0 and 3. Pine bark tannin treatment decreased (p<0.01) STNN populations in the litter by 0.6 log units compared to the controls. Wildtype E. coli populations were unaffected by tannin treatment (p>0.05). Ammonia accumulation decreased (p<0.01) 23% in tannin-treated litter compared to the control (2.8 ± 0.1 µmol/g). Conversely, the residual uric acid concentration was 1.6-fold higher (p=0.02) in litter treated with the pine bark tannin than in the control litter. Urea concentrations were unaffected by tannin treatment (p>0.05). Results suggest that pine bark tannin treatment may preserve uric acid and reduce ammonia volatilization in composted litter while aiding Salmonella control. |
| first_indexed | 2024-12-20T21:43:24Z |
| format | Article |
| id | doaj.art-4e5caedf997b4eeca6d113c1d611edc3 |
| institution | Directory Open Access Journal |
| issn | 1516-635X 1806-9061 |
| language | English |
| last_indexed | 2024-12-20T21:43:24Z |
| publisher | Fundação APINCO de Ciência e Tecnologia Avícolas |
| record_format | Article |
| series | Brazilian Journal of Poultry Science |
| spelling | doaj.art-4e5caedf997b4eeca6d113c1d611edc32022-12-21T19:25:44ZengFundação APINCO de Ciência e Tecnologia AvícolasBrazilian Journal of Poultry Science1516-635X1806-906122110.1590/1806-9061-2018-0911S1516-635X2020000100305Influence of Pine Bark Tannin on Bacterial Pathogens Growth and Nitrogen Compounds on Changes in Composted Poultry LitterC Arzola-AlavarezY Castillo-CastilloRC AndersonME HumeO Ruiz-BarreraBR MinA Arzola-RubioRC BeierJ Salinas-ChaviraABSTRACT To study the antimicrobial and uric acid-preserving activity of pine bark tannin on poultry litter composting, antibiotic-free wood chip-based poultry litter was distributed (11 g) to 50-mL conical centrifuge tubes and immediately amended with 1.3 mL0.4 M sodium phosphate buffer (control) or with 1.3 mL condensed tannins from pine bark (Pinus palustris; 9 % tannin wt/vol in water). All tubes (n = 3 tubes/treatment) were inoculated with a novobiocin and naladixic acid-resistant Salmo-nella typhimurium (STNN) to achieve 3.0 log10 CFU/g and incubated at 37oC for 3 days to simulate an initial compost period. Wildtype E. coli and the challenge STNN strain as well as concentrations of ammonia, uric acid and urea were measured on days 0 and 3. Pine bark tannin treatment decreased (p<0.01) STNN populations in the litter by 0.6 log units compared to the controls. Wildtype E. coli populations were unaffected by tannin treatment (p>0.05). Ammonia accumulation decreased (p<0.01) 23% in tannin-treated litter compared to the control (2.8 ± 0.1 µmol/g). Conversely, the residual uric acid concentration was 1.6-fold higher (p=0.02) in litter treated with the pine bark tannin than in the control litter. Urea concentrations were unaffected by tannin treatment (p>0.05). Results suggest that pine bark tannin treatment may preserve uric acid and reduce ammonia volatilization in composted litter while aiding Salmonella control.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000100305&lng=en&tlng=enpoultry littercondensed tanninsuric acidbacterial pathogens |
| spellingShingle | C Arzola-Alavarez Y Castillo-Castillo RC Anderson ME Hume O Ruiz-Barrera BR Min A Arzola-Rubio RC Beier J Salinas-Chavira Influence of Pine Bark Tannin on Bacterial Pathogens Growth and Nitrogen Compounds on Changes in Composted Poultry Litter Brazilian Journal of Poultry Science poultry litter condensed tannins uric acid bacterial pathogens |
| title | Influence of Pine Bark Tannin on Bacterial Pathogens Growth and Nitrogen Compounds on Changes in Composted Poultry Litter |
| title_full | Influence of Pine Bark Tannin on Bacterial Pathogens Growth and Nitrogen Compounds on Changes in Composted Poultry Litter |
| title_fullStr | Influence of Pine Bark Tannin on Bacterial Pathogens Growth and Nitrogen Compounds on Changes in Composted Poultry Litter |
| title_full_unstemmed | Influence of Pine Bark Tannin on Bacterial Pathogens Growth and Nitrogen Compounds on Changes in Composted Poultry Litter |
| title_short | Influence of Pine Bark Tannin on Bacterial Pathogens Growth and Nitrogen Compounds on Changes in Composted Poultry Litter |
| title_sort | influence of pine bark tannin on bacterial pathogens growth and nitrogen compounds on changes in composted poultry litter |
| topic | poultry litter condensed tannins uric acid bacterial pathogens |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000100305&lng=en&tlng=en |
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