Syringic acid induces cancer cell death in the presence of Cu (II) ions via pro-oxidant activity
Objective: To investigate the effects of syringic acid on HEK 293 and HepG2 cells in the absence and presence of exogenous Cu (II) and Fe (II) ions. Methods: The antiproliferative effects of syringic acid on HEK 293 and HepG2 cells in the absence and presence of exogenous Cu (II) and Fe (II) ions we...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2022-01-01
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| Series: | Asian Pacific Journal of Tropical Biomedicine |
| Subjects: | |
| Online Access: | http://www.apjtb.org/article.asp?issn=2221-1691;year=2022;volume=12;issue=6;spage=270;epage=278;aulast=Rashedinia |
| _version_ | 1818238037530771456 |
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| author | Marzieh Rashedinia Azita Nasrollahi Marzieh Shafaghat Shahrzad Momeni Forough Iranpak Jamileh Saberzadeh Rita Arabsolghar Zahra Sabahi |
| author_facet | Marzieh Rashedinia Azita Nasrollahi Marzieh Shafaghat Shahrzad Momeni Forough Iranpak Jamileh Saberzadeh Rita Arabsolghar Zahra Sabahi |
| author_sort | Marzieh Rashedinia |
| collection | DOAJ |
| description | Objective: To investigate the effects of syringic acid on HEK 293 and HepG2 cells in the absence and presence of exogenous Cu (II) and Fe (II) ions.
Methods: The antiproliferative effects of syringic acid on HEK 293 and HepG2 cells in the absence and presence of exogenous Cu (II) and Fe (II) ions were examined by MTT assay. Additionally, colony-forming, reactive oxidative species (ROS) generation, apoptosis induction, autophagy, mitochondrial membrane potential, and mitochondrial mass were investigated.
Results: At 24 and 72 h, no significant differences were observed in the viability of HepG2 cells between the control and syringic acid + Fe (II) groups. However, exposure of HepG2 cells to syringic acid + Cu (II) for 72 h reduced the cell viability significantly. Furthermore, ROS formation, induction of apoptosis, and autophagic vacuoles were significantly increased in HepG2 cells without marked changes in mitochondrial membrane potential and mitochondrial mass. Moreover, syringic acid + Cu (II) reduced the plating efficiency and surviving fraction significantly.
Conclusions: The combination of syringic acid with Cu (II) was toxic to cancer cells and showed pro-oxidant activity. In addition, this combination induced autophagy in cancer cells with less cytotoxic effects on normal cells, which is a potential candidate for the development of novel therapeutics towards cancer. |
| first_indexed | 2024-12-12T12:35:17Z |
| format | Article |
| id | doaj.art-4e5d8928a4dd4d148a6346f2323a37ac |
| institution | Directory Open Access Journal |
| issn | 2221-1691 2588-9222 |
| language | English |
| last_indexed | 2024-12-12T12:35:17Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj.art-4e5d8928a4dd4d148a6346f2323a37ac2022-12-22T00:24:20ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912588-92222022-01-0112627027810.4103/2221-1691.345519Syringic acid induces cancer cell death in the presence of Cu (II) ions via pro-oxidant activityMarzieh RashediniaAzita NasrollahiMarzieh ShafaghatShahrzad MomeniForough IranpakJamileh SaberzadehRita ArabsolgharZahra SabahiObjective: To investigate the effects of syringic acid on HEK 293 and HepG2 cells in the absence and presence of exogenous Cu (II) and Fe (II) ions. Methods: The antiproliferative effects of syringic acid on HEK 293 and HepG2 cells in the absence and presence of exogenous Cu (II) and Fe (II) ions were examined by MTT assay. Additionally, colony-forming, reactive oxidative species (ROS) generation, apoptosis induction, autophagy, mitochondrial membrane potential, and mitochondrial mass were investigated. Results: At 24 and 72 h, no significant differences were observed in the viability of HepG2 cells between the control and syringic acid + Fe (II) groups. However, exposure of HepG2 cells to syringic acid + Cu (II) for 72 h reduced the cell viability significantly. Furthermore, ROS formation, induction of apoptosis, and autophagic vacuoles were significantly increased in HepG2 cells without marked changes in mitochondrial membrane potential and mitochondrial mass. Moreover, syringic acid + Cu (II) reduced the plating efficiency and surviving fraction significantly. Conclusions: The combination of syringic acid with Cu (II) was toxic to cancer cells and showed pro-oxidant activity. In addition, this combination induced autophagy in cancer cells with less cytotoxic effects on normal cells, which is a potential candidate for the development of novel therapeutics towards cancer.http://www.apjtb.org/article.asp?issn=2221-1691;year=2022;volume=12;issue=6;spage=270;epage=278;aulast=Rashediniasyringic acid; copper; iron; cancer cell; pro- oxidant; autophagy |
| spellingShingle | Marzieh Rashedinia Azita Nasrollahi Marzieh Shafaghat Shahrzad Momeni Forough Iranpak Jamileh Saberzadeh Rita Arabsolghar Zahra Sabahi Syringic acid induces cancer cell death in the presence of Cu (II) ions via pro-oxidant activity Asian Pacific Journal of Tropical Biomedicine syringic acid; copper; iron; cancer cell; pro- oxidant; autophagy |
| title | Syringic acid induces cancer cell death in the presence of Cu (II) ions via pro-oxidant activity |
| title_full | Syringic acid induces cancer cell death in the presence of Cu (II) ions via pro-oxidant activity |
| title_fullStr | Syringic acid induces cancer cell death in the presence of Cu (II) ions via pro-oxidant activity |
| title_full_unstemmed | Syringic acid induces cancer cell death in the presence of Cu (II) ions via pro-oxidant activity |
| title_short | Syringic acid induces cancer cell death in the presence of Cu (II) ions via pro-oxidant activity |
| title_sort | syringic acid induces cancer cell death in the presence of cu ii ions via pro oxidant activity |
| topic | syringic acid; copper; iron; cancer cell; pro- oxidant; autophagy |
| url | http://www.apjtb.org/article.asp?issn=2221-1691;year=2022;volume=12;issue=6;spage=270;epage=278;aulast=Rashedinia |
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