Evidence of hydrocarbon nanodrops in highly ordered stratum corneum model membranes[S]
The stratum corneum (SC), the top layer of skin, dictates the rate of both water loss through the skin and absorption of exogenous molecules into the body. The crystalline organization of the lipids in the SC is believed to be a key feature associated with the very limited permeability of the skin....
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-01-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520339390 |
| _version_ | 1818898514952847360 |
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| author | Adrian Paz Ramos Gert Gooris Joke Bouwstra Michel Lafleur |
| author_facet | Adrian Paz Ramos Gert Gooris Joke Bouwstra Michel Lafleur |
| author_sort | Adrian Paz Ramos |
| collection | DOAJ |
| description | The stratum corneum (SC), the top layer of skin, dictates the rate of both water loss through the skin and absorption of exogenous molecules into the body. The crystalline organization of the lipids in the SC is believed to be a key feature associated with the very limited permeability of the skin. In this work, we characterized the organization of SC lipid models that include, as in native SC, cholesterol, a series of FFAs (saturated with C16-C24 chains), as well as a ceramide bearing an oleate chain-linked to a very long saturated acyl chain [N-melissoyl-oleoyloxy hexacosanoyl-D-erythro-sphingosine (Cer EOS)]. The latter is reported to be essential for the native SC lipid organization. Our 2H-NMR, infrared, and Raman spectroscopy data reveal that Cer EOS leads to the formation of highly disordered liquid domains in a solid/crystalline matrix. The lipid organization imposes steric constraint on Cer EOS oleate chains in such a way that these hydrocarbon nanodroplets remain in the liquid state down to −30°C. These findings modify the structural description of the SC substantially and propose a novel role of Cer EOS, as this lipid is a strong modulator of SC solid/liquid balance. |
| first_indexed | 2024-12-19T19:33:17Z |
| format | Article |
| id | doaj.art-4e5df26affc049c487f0a33e9c677f6d |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-19T19:33:17Z |
| publishDate | 2018-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-4e5df26affc049c487f0a33e9c677f6d2022-12-21T20:08:32ZengElsevierJournal of Lipid Research0022-22752018-01-01591137143Evidence of hydrocarbon nanodrops in highly ordered stratum corneum model membranes[S]Adrian Paz Ramos0Gert Gooris1Joke Bouwstra2Michel Lafleur3Department of Chemistry, Université de Montréal, Montréal, Québec H3C 3J7, CanadaDepartment of Drug Delivery Technology, Leiden Academic Centre for Drug Research, 2333 CC Leiden, The NetherlandsDepartment of Drug Delivery Technology, Leiden Academic Centre for Drug Research, 2333 CC Leiden, The NetherlandsDepartment of Chemistry, Université de Montréal, Montréal, Québec H3C 3J7, Canada; To whom correspondence should be addressed.The stratum corneum (SC), the top layer of skin, dictates the rate of both water loss through the skin and absorption of exogenous molecules into the body. The crystalline organization of the lipids in the SC is believed to be a key feature associated with the very limited permeability of the skin. In this work, we characterized the organization of SC lipid models that include, as in native SC, cholesterol, a series of FFAs (saturated with C16-C24 chains), as well as a ceramide bearing an oleate chain-linked to a very long saturated acyl chain [N-melissoyl-oleoyloxy hexacosanoyl-D-erythro-sphingosine (Cer EOS)]. The latter is reported to be essential for the native SC lipid organization. Our 2H-NMR, infrared, and Raman spectroscopy data reveal that Cer EOS leads to the formation of highly disordered liquid domains in a solid/crystalline matrix. The lipid organization imposes steric constraint on Cer EOS oleate chains in such a way that these hydrocarbon nanodroplets remain in the liquid state down to −30°C. These findings modify the structural description of the SC substantially and propose a novel role of Cer EOS, as this lipid is a strong modulator of SC solid/liquid balance.http://www.sciencedirect.com/science/article/pii/S0022227520339390ceramide2H-nuclear magnetic resonanceIR spectroscopyphase behaviorlipidsskin |
| spellingShingle | Adrian Paz Ramos Gert Gooris Joke Bouwstra Michel Lafleur Evidence of hydrocarbon nanodrops in highly ordered stratum corneum model membranes[S] Journal of Lipid Research ceramide 2H-nuclear magnetic resonance IR spectroscopy phase behavior lipids skin |
| title | Evidence of hydrocarbon nanodrops in highly ordered stratum corneum model membranes[S] |
| title_full | Evidence of hydrocarbon nanodrops in highly ordered stratum corneum model membranes[S] |
| title_fullStr | Evidence of hydrocarbon nanodrops in highly ordered stratum corneum model membranes[S] |
| title_full_unstemmed | Evidence of hydrocarbon nanodrops in highly ordered stratum corneum model membranes[S] |
| title_short | Evidence of hydrocarbon nanodrops in highly ordered stratum corneum model membranes[S] |
| title_sort | evidence of hydrocarbon nanodrops in highly ordered stratum corneum model membranes s |
| topic | ceramide 2H-nuclear magnetic resonance IR spectroscopy phase behavior lipids skin |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520339390 |
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