In vitro re-hardening of artificial enamel caries lesions using enamel matrix proteins or self-assembling peptides
ABSTRACT Objectives To assess the re-hardening potential of enamel matrix derivatives (EMD) and self-assembling peptides in vitro, hypothesizing that these materials may increase the mineralization of artificial carious lesions and improve hardness profiles. Material and Methods Forty-eight enam...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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University of São Paulo
2016-02-01
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| Series: | Journal of Applied Oral Science |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572016000100031&lng=en&tlng=en |
| _version_ | 1819239301921112064 |
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| author | Patrick Schmidlin Katja Zobrist Thomas Attin Florian Wegehaupt |
| author_facet | Patrick Schmidlin Katja Zobrist Thomas Attin Florian Wegehaupt |
| author_sort | Patrick Schmidlin |
| collection | DOAJ |
| description | ABSTRACT Objectives To assess the re-hardening potential of enamel matrix derivatives (EMD) and self-assembling peptides in vitro, hypothesizing that these materials may increase the mineralization of artificial carious lesions and improve hardness profiles. Material and Methods Forty-eight enamel samples were prepared from extracted bovine lower central incisors. After embedding and polishing, nail varnish was applied, leaving a defined test area. One third of this area was covered with a flowable composite (non-demineralized control). The remaining area was demineralized in an acidic buffer solution for 18 d to simulate a carious lesion. Half the demineralized area was then covered with composite (demineralized control), while the last third was left open for three test and one control treatments: (A) Application of enamel-matrix proteins (EMD - lyophilized protein fractions dissolved in acetic acid, Straumann), (B) self-assembling peptides (SAP, Curodont), or (C) amine fluoride solution (Am-F, GABA) for 5 min each. Untreated samples (D) served as control. After treatment, samples were immersed in artificial saliva for four weeks (remineralization phase) and microhardness (Knoop) depth profiles (25-300 µm) were obtained at sections. Two-way ANOVA was calculated to determine differences between the areas (re-hardening or softening). Results Decalcification resulted in significant softening of the subsurface enamel in all groups (A-D). A significant re-hardening up to 125 µm was observed in the EMD and SAP groups. Conclusions This study showed that EMD and SAP were able to improve the hardness profiles when applied to deep demineralized artificial lesions. However, further research is needed to verify and improve this observed effect. |
| first_indexed | 2024-12-23T13:49:57Z |
| format | Article |
| id | doaj.art-4e62804529ca4ec680b7eae9130004fa |
| institution | Directory Open Access Journal |
| issn | 1678-7765 |
| language | English |
| last_indexed | 2024-12-23T13:49:57Z |
| publishDate | 2016-02-01 |
| publisher | University of São Paulo |
| record_format | Article |
| series | Journal of Applied Oral Science |
| spelling | doaj.art-4e62804529ca4ec680b7eae9130004fa2022-12-21T17:44:37ZengUniversity of São PauloJournal of Applied Oral Science1678-77652016-02-01241313610.1590/1678-775720150352S1678-77572016000100031In vitro re-hardening of artificial enamel caries lesions using enamel matrix proteins or self-assembling peptidesPatrick SchmidlinKatja ZobristThomas AttinFlorian WegehauptABSTRACT Objectives To assess the re-hardening potential of enamel matrix derivatives (EMD) and self-assembling peptides in vitro, hypothesizing that these materials may increase the mineralization of artificial carious lesions and improve hardness profiles. Material and Methods Forty-eight enamel samples were prepared from extracted bovine lower central incisors. After embedding and polishing, nail varnish was applied, leaving a defined test area. One third of this area was covered with a flowable composite (non-demineralized control). The remaining area was demineralized in an acidic buffer solution for 18 d to simulate a carious lesion. Half the demineralized area was then covered with composite (demineralized control), while the last third was left open for three test and one control treatments: (A) Application of enamel-matrix proteins (EMD - lyophilized protein fractions dissolved in acetic acid, Straumann), (B) self-assembling peptides (SAP, Curodont), or (C) amine fluoride solution (Am-F, GABA) for 5 min each. Untreated samples (D) served as control. After treatment, samples were immersed in artificial saliva for four weeks (remineralization phase) and microhardness (Knoop) depth profiles (25-300 µm) were obtained at sections. Two-way ANOVA was calculated to determine differences between the areas (re-hardening or softening). Results Decalcification resulted in significant softening of the subsurface enamel in all groups (A-D). A significant re-hardening up to 125 µm was observed in the EMD and SAP groups. Conclusions This study showed that EMD and SAP were able to improve the hardness profiles when applied to deep demineralized artificial lesions. However, further research is needed to verify and improve this observed effect.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572016000100031&lng=en&tlng=enTooth remineralizationEnamelHardness |
| spellingShingle | Patrick Schmidlin Katja Zobrist Thomas Attin Florian Wegehaupt In vitro re-hardening of artificial enamel caries lesions using enamel matrix proteins or self-assembling peptides Journal of Applied Oral Science Tooth remineralization Enamel Hardness |
| title | In vitro re-hardening of artificial enamel caries lesions using enamel matrix proteins or self-assembling peptides |
| title_full | In vitro re-hardening of artificial enamel caries lesions using enamel matrix proteins or self-assembling peptides |
| title_fullStr | In vitro re-hardening of artificial enamel caries lesions using enamel matrix proteins or self-assembling peptides |
| title_full_unstemmed | In vitro re-hardening of artificial enamel caries lesions using enamel matrix proteins or self-assembling peptides |
| title_short | In vitro re-hardening of artificial enamel caries lesions using enamel matrix proteins or self-assembling peptides |
| title_sort | in vitro re hardening of artificial enamel caries lesions using enamel matrix proteins or self assembling peptides |
| topic | Tooth remineralization Enamel Hardness |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572016000100031&lng=en&tlng=en |
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