Ultraviolet metasurface-assisted photoacoustic microscopy with great enhancement in DOF for fast histology imaging
Pathology interpretations of tissue rely on the gold standard of histology imaging, potentially hampering timely access to critical information for diagnosis and management of neoplasms because of tedious sample preparations. Slide-free capture of cell nuclei in unprocessed specimens without stainin...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | Photoacoustics |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213597923000782 |
| _version_ | 1797731014571720704 |
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| author | Wei Song Changkui Guo Yuting Zhao Ya-chao Wang Siwei Zhu Changjun Min Xiaocong Yuan |
| author_facet | Wei Song Changkui Guo Yuting Zhao Ya-chao Wang Siwei Zhu Changjun Min Xiaocong Yuan |
| author_sort | Wei Song |
| collection | DOAJ |
| description | Pathology interpretations of tissue rely on the gold standard of histology imaging, potentially hampering timely access to critical information for diagnosis and management of neoplasms because of tedious sample preparations. Slide-free capture of cell nuclei in unprocessed specimens without staining is preferable; however, inevitable irregular surfaces in fresh tissues results in limitations. An ultraviolet metasurface with the ability to generate an ultraviolet optical focus maintaining < 1.1-µm in lateral resolution and ∼290 µm in depth of field (DOF) is proposed for fast, high resolution, label-free photoacoustic histological imaging of unprocessed tissues with uneven surfaces. Microanatomical characteristics of the cell nuclei can be observed, as demonstrated by the mouse brain samples that were cut by hand and a ∼3 × 3-mm2 field of view was imaged in ∼27 min. Therefore, ultraviolet metasurface-assisted photoacoustic microscopy is anticipated to benefit intraoperative pathological assessments and basic scientific research by alleviating laborious tissue preparations. |
| first_indexed | 2024-03-12T11:53:23Z |
| format | Article |
| id | doaj.art-4e6589525ee74788b1083fb58557758f |
| institution | Directory Open Access Journal |
| issn | 2213-5979 |
| language | English |
| last_indexed | 2024-03-12T11:53:23Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Photoacoustics |
| spelling | doaj.art-4e6589525ee74788b1083fb58557758f2023-08-31T05:02:42ZengElsevierPhotoacoustics2213-59792023-08-0132100525Ultraviolet metasurface-assisted photoacoustic microscopy with great enhancement in DOF for fast histology imagingWei Song0Changkui Guo1Yuting Zhao2Ya-chao Wang3Siwei Zhu4Changjun Min5Xiaocong Yuan6Nanophotonics Research Center, Shenzhen Key Laboratory of Micro-Scale Optical Information Technology, Institute of Microscale Optoelectronics & State Key Laboratory of Radio Frequency Heterogeneous, Shenzhen University, Shenzhen 518060, China; Corresponding authors.Nanophotonics Research Center, Shenzhen Key Laboratory of Micro-Scale Optical Information Technology, Institute of Microscale Optoelectronics & State Key Laboratory of Radio Frequency Heterogeneous, Shenzhen University, Shenzhen 518060, ChinaNanophotonics Research Center, Shenzhen Key Laboratory of Micro-Scale Optical Information Technology, Institute of Microscale Optoelectronics & State Key Laboratory of Radio Frequency Heterogeneous, Shenzhen University, Shenzhen 518060, ChinaDepart of Neurosurgery, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, Shenzhen 518060, ChinaThe Institute of Translational Medicine, Tianjin Union Medical Center of Nankai University, Tianjin 300121, ChinaNanophotonics Research Center, Shenzhen Key Laboratory of Micro-Scale Optical Information Technology, Institute of Microscale Optoelectronics & State Key Laboratory of Radio Frequency Heterogeneous, Shenzhen University, Shenzhen 518060, China; Corresponding authors.Nanophotonics Research Center, Shenzhen Key Laboratory of Micro-Scale Optical Information Technology, Institute of Microscale Optoelectronics & State Key Laboratory of Radio Frequency Heterogeneous, Shenzhen University, Shenzhen 518060, China; Research Center for Humanoid Sensing, Zhejiang Laboratory, Hangzhou 311100, China; Corresponding author at: Nanophotonics Research Center, Shenzhen Key Laboratory of Micro-Scale Optical Information Technology, Institute of Microscale Optoelectronics & State Key Laboratory of Radio Frequency Heterogeneous, Shenzhen University, Shenzhen 518060, China.Pathology interpretations of tissue rely on the gold standard of histology imaging, potentially hampering timely access to critical information for diagnosis and management of neoplasms because of tedious sample preparations. Slide-free capture of cell nuclei in unprocessed specimens without staining is preferable; however, inevitable irregular surfaces in fresh tissues results in limitations. An ultraviolet metasurface with the ability to generate an ultraviolet optical focus maintaining < 1.1-µm in lateral resolution and ∼290 µm in depth of field (DOF) is proposed for fast, high resolution, label-free photoacoustic histological imaging of unprocessed tissues with uneven surfaces. Microanatomical characteristics of the cell nuclei can be observed, as demonstrated by the mouse brain samples that were cut by hand and a ∼3 × 3-mm2 field of view was imaged in ∼27 min. Therefore, ultraviolet metasurface-assisted photoacoustic microscopy is anticipated to benefit intraoperative pathological assessments and basic scientific research by alleviating laborious tissue preparations.http://www.sciencedirect.com/science/article/pii/S2213597923000782Ultraviolet metasurfacePhotoacoustic microscopyLabel-free histology imagingEnlarged focal depthUnprocessed tissue |
| spellingShingle | Wei Song Changkui Guo Yuting Zhao Ya-chao Wang Siwei Zhu Changjun Min Xiaocong Yuan Ultraviolet metasurface-assisted photoacoustic microscopy with great enhancement in DOF for fast histology imaging Photoacoustics Ultraviolet metasurface Photoacoustic microscopy Label-free histology imaging Enlarged focal depth Unprocessed tissue |
| title | Ultraviolet metasurface-assisted photoacoustic microscopy with great enhancement in DOF for fast histology imaging |
| title_full | Ultraviolet metasurface-assisted photoacoustic microscopy with great enhancement in DOF for fast histology imaging |
| title_fullStr | Ultraviolet metasurface-assisted photoacoustic microscopy with great enhancement in DOF for fast histology imaging |
| title_full_unstemmed | Ultraviolet metasurface-assisted photoacoustic microscopy with great enhancement in DOF for fast histology imaging |
| title_short | Ultraviolet metasurface-assisted photoacoustic microscopy with great enhancement in DOF for fast histology imaging |
| title_sort | ultraviolet metasurface assisted photoacoustic microscopy with great enhancement in dof for fast histology imaging |
| topic | Ultraviolet metasurface Photoacoustic microscopy Label-free histology imaging Enlarged focal depth Unprocessed tissue |
| url | http://www.sciencedirect.com/science/article/pii/S2213597923000782 |
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