Antimicrobial Activity and Toxicity of Analogs of Wasp Venom EMP Peptides. Potential Influence of Oxidized Methionine
The antibiotic and toxic properties for four synthetic analogs of eumenine mastoparan peptides (EMP) have been tested. These properties were compared to two natural peptides found in the venom of solitary wasps <i>Anterhynchium flavomarginatum micado</i> (natural peptide EMP-AF) and <...
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MDPI AG
2021-10-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/10/10/1208 |
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| author | Roberto de la Salud Bea Lily J. North Sakura Horiuchi Elaine R. Frawley Qian Shen |
| author_facet | Roberto de la Salud Bea Lily J. North Sakura Horiuchi Elaine R. Frawley Qian Shen |
| author_sort | Roberto de la Salud Bea |
| collection | DOAJ |
| description | The antibiotic and toxic properties for four synthetic analogs of eumenine mastoparan peptides (EMP) have been tested. These properties were compared to two natural peptides found in the venom of solitary wasps <i>Anterhynchium flavomarginatum micado</i> (natural peptide EMP-AF) and <i>Eumenes rubrofemoratus</i> (natural peptide EMP-ER), respectively. Only EMP-AF-OR showed concentration-dependent growth inhibition against all bacterial species tested. Gram positive species had MIC values of 10 μg/mL for <i>B. subtilis</i> and 25 μg/mL for <i>S. aureus.</i> Gram negative species had MIC values of 25 μg/mL for <i>E. coli</i> and 200 μg/mL for <i>P. aeruginosa</i>. Of the other tested peptides, EMP-ER-D2K2 also showed activity and inhibited growth of <i>Bacillus subtilis</i> in a concentration-dependent manner at 200 μg/mL. Peptide EMP-ER-OR reduced the final density of <i>Escherichia coli</i> and <i>B. subtilis</i> cultures but did not impact their growth kinetics. Peptides EMP-AF-OR, EMP-ER-OR, and EMP-ER-D2K2 showed limited antifungal activity against <i>Candida albicans</i> or <i>Histoplasma capsulatum</i>. The hemolytic activity of the analogs were moderated though reports of the natural peptides, especially EMP-AF-OR, already showed low toxicity against erythrocytes. These results are discussed in the context of the potential influence of oxidized methionine on EMP activity. |
| first_indexed | 2024-03-10T06:46:09Z |
| format | Article |
| id | doaj.art-4e6aafc4118c4372bf7f387b31633c63 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T06:46:09Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-4e6aafc4118c4372bf7f387b31633c632023-11-22T17:13:46ZengMDPI AGAntibiotics2079-63822021-10-011010120810.3390/antibiotics10101208Antimicrobial Activity and Toxicity of Analogs of Wasp Venom EMP Peptides. Potential Influence of Oxidized MethionineRoberto de la Salud Bea0Lily J. North1Sakura Horiuchi2Elaine R. Frawley3Qian Shen4Department of Chemistry, Rhodes College, 2000 North Parkway, Memphis, TN 38112, USADepartment of Chemistry, The University of Arizona, Tucson, AZ 85721, USASchool of Medicine and Health Sciences, George Washington University, 2300 I St., NW, Washington, DC 20052, USADepartment of Biology, Rhodes College, 2000 North Parkway, Memphis, TN 38112, USADepartment of Biology, Rhodes College, 2000 North Parkway, Memphis, TN 38112, USAThe antibiotic and toxic properties for four synthetic analogs of eumenine mastoparan peptides (EMP) have been tested. These properties were compared to two natural peptides found in the venom of solitary wasps <i>Anterhynchium flavomarginatum micado</i> (natural peptide EMP-AF) and <i>Eumenes rubrofemoratus</i> (natural peptide EMP-ER), respectively. Only EMP-AF-OR showed concentration-dependent growth inhibition against all bacterial species tested. Gram positive species had MIC values of 10 μg/mL for <i>B. subtilis</i> and 25 μg/mL for <i>S. aureus.</i> Gram negative species had MIC values of 25 μg/mL for <i>E. coli</i> and 200 μg/mL for <i>P. aeruginosa</i>. Of the other tested peptides, EMP-ER-D2K2 also showed activity and inhibited growth of <i>Bacillus subtilis</i> in a concentration-dependent manner at 200 μg/mL. Peptide EMP-ER-OR reduced the final density of <i>Escherichia coli</i> and <i>B. subtilis</i> cultures but did not impact their growth kinetics. Peptides EMP-AF-OR, EMP-ER-OR, and EMP-ER-D2K2 showed limited antifungal activity against <i>Candida albicans</i> or <i>Histoplasma capsulatum</i>. The hemolytic activity of the analogs were moderated though reports of the natural peptides, especially EMP-AF-OR, already showed low toxicity against erythrocytes. These results are discussed in the context of the potential influence of oxidized methionine on EMP activity.https://www.mdpi.com/2079-6382/10/10/1208antimicrobial peptidesvenomwaspmethionine |
| spellingShingle | Roberto de la Salud Bea Lily J. North Sakura Horiuchi Elaine R. Frawley Qian Shen Antimicrobial Activity and Toxicity of Analogs of Wasp Venom EMP Peptides. Potential Influence of Oxidized Methionine Antibiotics antimicrobial peptides venom wasp methionine |
| title | Antimicrobial Activity and Toxicity of Analogs of Wasp Venom EMP Peptides. Potential Influence of Oxidized Methionine |
| title_full | Antimicrobial Activity and Toxicity of Analogs of Wasp Venom EMP Peptides. Potential Influence of Oxidized Methionine |
| title_fullStr | Antimicrobial Activity and Toxicity of Analogs of Wasp Venom EMP Peptides. Potential Influence of Oxidized Methionine |
| title_full_unstemmed | Antimicrobial Activity and Toxicity of Analogs of Wasp Venom EMP Peptides. Potential Influence of Oxidized Methionine |
| title_short | Antimicrobial Activity and Toxicity of Analogs of Wasp Venom EMP Peptides. Potential Influence of Oxidized Methionine |
| title_sort | antimicrobial activity and toxicity of analogs of wasp venom emp peptides potential influence of oxidized methionine |
| topic | antimicrobial peptides venom wasp methionine |
| url | https://www.mdpi.com/2079-6382/10/10/1208 |
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