Knockout of lipoprotein lipase with CRISPR/Cas9 causes severe developmental defects and affects lipid deposition in Japanese medaka (Oryzias latipes)
In mammals, lipoprotein lipase (LPL) has been found to play an important role in lipid mentalism and deposition. LPL deficiency in humans (Homo sapiens) and mice (Mus musculus) tends to cause hypertriglyceridemia. The lpl gene is not expressed in adult mammalian liver, but is in adult fish liver. Th...
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| Format: | Article |
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KeAi Communications Co. Ltd.
2022-05-01
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| Series: | Water Biology and Security |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S277273512200052X |
| _version_ | 1797837699855417344 |
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| author | Hexiong Feng Xu-Fang Liang |
| author_facet | Hexiong Feng Xu-Fang Liang |
| author_sort | Hexiong Feng |
| collection | DOAJ |
| description | In mammals, lipoprotein lipase (LPL) has been found to play an important role in lipid mentalism and deposition. LPL deficiency in humans (Homo sapiens) and mice (Mus musculus) tends to cause hypertriglyceridemia. The lpl gene is not expressed in adult mammalian liver, but is in adult fish liver. The functions provided by the lpl gene are diverse in vertebrates. Here, we knocked out the lpl gene in Japanese medaka (Oryzias latipes) with the CRISPR/Cas9 system. The lpl-knockout (KO) homozygous individuals showed severe developmental defects with an extremely emaciated and deformed body and only accounted for about 5% of the F2 fish. This is consistent with the findings in mice but disaccords with the results for zebrafish (Danio rerio). Compared with wild-type (WT) madaka, the mRNA level of lpl in lpl-KO heterozygous mutant was significantly higher in the muscle, showed no significant difference in the liver, and was significantly lower in the heart. Under lpl heterozygous deficiency, the relative area of Oil Red O and triglycerides (TG) level in the liver, heart and muscle tissue covaried with levels of lpl mRNA in medaka. The lpl heterozygous deficiency did not affect the levels of TG, low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C) and total cholesterol (TC) in the plasma of medaka, which is inconsistent with the findings in mammals. In general, the lpl gene plays an important role in the growth and development and is closely related to lipid deposition of medaka. |
| first_indexed | 2024-04-09T15:30:01Z |
| format | Article |
| id | doaj.art-4e6b1d4236674ad4ac2fdbfda996a76d |
| institution | Directory Open Access Journal |
| issn | 2772-7351 |
| language | English |
| last_indexed | 2024-04-09T15:30:01Z |
| publishDate | 2022-05-01 |
| publisher | KeAi Communications Co. Ltd. |
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| series | Water Biology and Security |
| spelling | doaj.art-4e6b1d4236674ad4ac2fdbfda996a76d2023-04-28T08:57:25ZengKeAi Communications Co. Ltd.Water Biology and Security2772-73512022-05-0112100038Knockout of lipoprotein lipase with CRISPR/Cas9 causes severe developmental defects and affects lipid deposition in Japanese medaka (Oryzias latipes)Hexiong Feng0Xu-Fang Liang1College of Fisheries and Chinese Perch Research Center, Huazhong Agricultural University, Wuhan, 430070, China; Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Wuhan, 430070, ChinaCollege of Fisheries and Chinese Perch Research Center, Huazhong Agricultural University, Wuhan, 430070, China; Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Wuhan, 430070, China; Corresponding author. College of Fisheries, Huazhong Agricultural University, No.1, Shizishan Street, Hongshan District, Wuhan, Hubei Province, 430070, China.In mammals, lipoprotein lipase (LPL) has been found to play an important role in lipid mentalism and deposition. LPL deficiency in humans (Homo sapiens) and mice (Mus musculus) tends to cause hypertriglyceridemia. The lpl gene is not expressed in adult mammalian liver, but is in adult fish liver. The functions provided by the lpl gene are diverse in vertebrates. Here, we knocked out the lpl gene in Japanese medaka (Oryzias latipes) with the CRISPR/Cas9 system. The lpl-knockout (KO) homozygous individuals showed severe developmental defects with an extremely emaciated and deformed body and only accounted for about 5% of the F2 fish. This is consistent with the findings in mice but disaccords with the results for zebrafish (Danio rerio). Compared with wild-type (WT) madaka, the mRNA level of lpl in lpl-KO heterozygous mutant was significantly higher in the muscle, showed no significant difference in the liver, and was significantly lower in the heart. Under lpl heterozygous deficiency, the relative area of Oil Red O and triglycerides (TG) level in the liver, heart and muscle tissue covaried with levels of lpl mRNA in medaka. The lpl heterozygous deficiency did not affect the levels of TG, low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C) and total cholesterol (TC) in the plasma of medaka, which is inconsistent with the findings in mammals. In general, the lpl gene plays an important role in the growth and development and is closely related to lipid deposition of medaka.http://www.sciencedirect.com/science/article/pii/S277273512200052XLipoprotein lipaseKnockoutLipidJapanese medaka (Oryzias latipes) |
| spellingShingle | Hexiong Feng Xu-Fang Liang Knockout of lipoprotein lipase with CRISPR/Cas9 causes severe developmental defects and affects lipid deposition in Japanese medaka (Oryzias latipes) Water Biology and Security Lipoprotein lipase Knockout Lipid Japanese medaka (Oryzias latipes) |
| title | Knockout of lipoprotein lipase with CRISPR/Cas9 causes severe developmental defects and affects lipid deposition in Japanese medaka (Oryzias latipes) |
| title_full | Knockout of lipoprotein lipase with CRISPR/Cas9 causes severe developmental defects and affects lipid deposition in Japanese medaka (Oryzias latipes) |
| title_fullStr | Knockout of lipoprotein lipase with CRISPR/Cas9 causes severe developmental defects and affects lipid deposition in Japanese medaka (Oryzias latipes) |
| title_full_unstemmed | Knockout of lipoprotein lipase with CRISPR/Cas9 causes severe developmental defects and affects lipid deposition in Japanese medaka (Oryzias latipes) |
| title_short | Knockout of lipoprotein lipase with CRISPR/Cas9 causes severe developmental defects and affects lipid deposition in Japanese medaka (Oryzias latipes) |
| title_sort | knockout of lipoprotein lipase with crispr cas9 causes severe developmental defects and affects lipid deposition in japanese medaka oryzias latipes |
| topic | Lipoprotein lipase Knockout Lipid Japanese medaka (Oryzias latipes) |
| url | http://www.sciencedirect.com/science/article/pii/S277273512200052X |
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