Protective Effects of Melon Extracts on Bone Strength, Mineralization, and Metabolism in Rats with Ovariectomy-Induced Osteoporosis
A polyphenolic extract from melon (<i>Cucumis melo</i> L.), as a potential source of natural antioxidants, has been reported to have a positive effect on osteoblast activity. In this study, the protective effects of heat-treated melon extract (ECO-A) on bone strength, mineralization, and...
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MDPI AG
2019-08-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/8/8/306 |
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| author | Bongju Kim Sung-Ho Lee Su-Jin Song Won Hyeon Kim Eun-Sung Song Jae-Chang Lee Sung-Jae Lee Dong-Wook Han Jong-Ho Lee |
| author_facet | Bongju Kim Sung-Ho Lee Su-Jin Song Won Hyeon Kim Eun-Sung Song Jae-Chang Lee Sung-Jae Lee Dong-Wook Han Jong-Ho Lee |
| author_sort | Bongju Kim |
| collection | DOAJ |
| description | A polyphenolic extract from melon (<i>Cucumis melo</i> L.), as a potential source of natural antioxidants, has been reported to have a positive effect on osteoblast activity. In this study, the protective effects of heat-treated melon extract (ECO-A) on bone strength, mineralization, and metabolism were examined in osteoporotic rat models. Osteoporosis was induced by ovariectomy (OVX) in female rats and then maintained for 8 weeks, along with the ingestion of phosphate-buffered saline (PBS, OVXP) or ECO-A (OVXE) for an additional 4 weeks. At a pre-determined timepoint, bone strengths, as well as bone mineral contents (BMC) and the density (BMD) of femurs and/or lumbar spines extracted from each animal, were measured by a mechanical test and dual-energy X-ray absorptiometry, respectively. Moreover, several biochemical markers for bone turnover were analyzed by respective colorimetric assay kits in addition to clinical analyses. The maximum load and stiffness of femurs from the OVXE group were found to be significantly higher than the other groups. Furthermore, the OVXE group showed significantly higher BMC, BMD, and bone volume than the OVX and OVXP groups, which were comparable to the non-OVX (sham) group. The levels of bone formation and resorption markers in the OVXE group were similar to the sham group, but significantly different from other groups. In conclusion, these results suggest that ECO-A can play potentially positive roles in the protection of bone loss in rats with OVX-induced osteoporosis. |
| first_indexed | 2024-03-12T09:43:03Z |
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| id | doaj.art-4e6b39e97a3543d08ed7aca05c8390f6 |
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| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-12T09:43:03Z |
| publishDate | 2019-08-01 |
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| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-4e6b39e97a3543d08ed7aca05c8390f62023-09-02T13:04:56ZengMDPI AGAntioxidants2076-39212019-08-018830610.3390/antiox8080306antiox8080306Protective Effects of Melon Extracts on Bone Strength, Mineralization, and Metabolism in Rats with Ovariectomy-Induced OsteoporosisBongju Kim0Sung-Ho Lee1Su-Jin Song2Won Hyeon Kim3Eun-Sung Song4Jae-Chang Lee5Sung-Jae Lee6Dong-Wook Han7Jong-Ho Lee8Dental Life Science Research Institute & Clinical Translational Research Center for Dental Science, Seoul National University Dental Hospital, Seoul 03080, KoreaDental Life Science Research Institute & Clinical Translational Research Center for Dental Science, Seoul National University Dental Hospital, Seoul 03080, KoreaDepartment of Cogno-Mechatronics Engineering, College of Nanoscience & Nanotechnology, Pusan National University, Busan 46241, KoreaDental Life Science Research Institute & Clinical Translational Research Center for Dental Science, Seoul National University Dental Hospital, Seoul 03080, KoreaDental Life Science Research Institute & Clinical Translational Research Center for Dental Science, Seoul National University Dental Hospital, Seoul 03080, KoreaBio-based Chemistry Research Center, Korea Research Institute of Chemical Technology, Ulsan 44429, KoreaDepartment of Biomedical Engineering, Inje University, Gimhae 50834, KoreaDepartment of Cogno-Mechatronics Engineering, College of Nanoscience & Nanotechnology, Pusan National University, Busan 46241, KoreaDental Life Science Research Institute & Clinical Translational Research Center for Dental Science, Seoul National University Dental Hospital, Seoul 03080, KoreaA polyphenolic extract from melon (<i>Cucumis melo</i> L.), as a potential source of natural antioxidants, has been reported to have a positive effect on osteoblast activity. In this study, the protective effects of heat-treated melon extract (ECO-A) on bone strength, mineralization, and metabolism were examined in osteoporotic rat models. Osteoporosis was induced by ovariectomy (OVX) in female rats and then maintained for 8 weeks, along with the ingestion of phosphate-buffered saline (PBS, OVXP) or ECO-A (OVXE) for an additional 4 weeks. At a pre-determined timepoint, bone strengths, as well as bone mineral contents (BMC) and the density (BMD) of femurs and/or lumbar spines extracted from each animal, were measured by a mechanical test and dual-energy X-ray absorptiometry, respectively. Moreover, several biochemical markers for bone turnover were analyzed by respective colorimetric assay kits in addition to clinical analyses. The maximum load and stiffness of femurs from the OVXE group were found to be significantly higher than the other groups. Furthermore, the OVXE group showed significantly higher BMC, BMD, and bone volume than the OVX and OVXP groups, which were comparable to the non-OVX (sham) group. The levels of bone formation and resorption markers in the OVXE group were similar to the sham group, but significantly different from other groups. In conclusion, these results suggest that ECO-A can play potentially positive roles in the protection of bone loss in rats with OVX-induced osteoporosis.https://www.mdpi.com/2076-3921/8/8/306melon extractantioxidantosteoporosisbone strengthbone mineralizationbone metabolism |
| spellingShingle | Bongju Kim Sung-Ho Lee Su-Jin Song Won Hyeon Kim Eun-Sung Song Jae-Chang Lee Sung-Jae Lee Dong-Wook Han Jong-Ho Lee Protective Effects of Melon Extracts on Bone Strength, Mineralization, and Metabolism in Rats with Ovariectomy-Induced Osteoporosis Antioxidants melon extract antioxidant osteoporosis bone strength bone mineralization bone metabolism |
| title | Protective Effects of Melon Extracts on Bone Strength, Mineralization, and Metabolism in Rats with Ovariectomy-Induced Osteoporosis |
| title_full | Protective Effects of Melon Extracts on Bone Strength, Mineralization, and Metabolism in Rats with Ovariectomy-Induced Osteoporosis |
| title_fullStr | Protective Effects of Melon Extracts on Bone Strength, Mineralization, and Metabolism in Rats with Ovariectomy-Induced Osteoporosis |
| title_full_unstemmed | Protective Effects of Melon Extracts on Bone Strength, Mineralization, and Metabolism in Rats with Ovariectomy-Induced Osteoporosis |
| title_short | Protective Effects of Melon Extracts on Bone Strength, Mineralization, and Metabolism in Rats with Ovariectomy-Induced Osteoporosis |
| title_sort | protective effects of melon extracts on bone strength mineralization and metabolism in rats with ovariectomy induced osteoporosis |
| topic | melon extract antioxidant osteoporosis bone strength bone mineralization bone metabolism |
| url | https://www.mdpi.com/2076-3921/8/8/306 |
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