The Association between Cyclin D1 (CCND1) rs9344 AA Genotype and Increased Risk of Colorectal Cancer in An Iranian Population
Background: Colorectal cancer (CRC) is a globally growing disease with a steady decrease in the age of incidence. Pathogenesis of this cancer stems from a complex interaction between environmental factors and genetic predisposition. Among genetic factors, high activity of cyclin D1gene is promine...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Shiraz University of Medical Sciences
2020-07-01
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| Series: | Middle East Journal of Cancer |
| Subjects: | |
| Online Access: | https://mejc.sums.ac.ir/article_46727.html |
| Summary: | Background: Colorectal cancer (CRC) is a globally growing disease with a steady
decrease in the age of incidence. Pathogenesis of this cancer stems from a complex
interaction between environmental factors and genetic predisposition. Among genetic
factors, high activity of cyclin D1gene is prominent. A polymorphism (G870A) in
exon 4 of cyclin D1 is responsible for a variant transcript with longer half-life and
may culminate in uncontrollable cellular growth, thereby contributing to cancer
development.
Method: This case-control study evaluated the frequency of CCND1 G870A
polymorphism and risk of sporadic CRC in an Iranian population. The study population
comprised 50 CRC patients and 50 CRC-free controls selected on the basis of
colonoscopy examination. For genotyping, we performed polymerase chain reaction
– restriction fragment length polymorphism analysis (PCR-RFLP).
Result: AA genotype frequencies compared to GA+GG genotype frequencies
between cases and controls showed that AA genotype frequency in the case group
was significantly higher than the control group (AA vs. GG + GA: OR= 2.25, 95%
CI: 1.13-5.54, P=0.04). Allele A frequency was 57% in patients and 46% in healthy
subjects. Statistical analysis showed that the odds ratio of carriers with allele A for
risk of CRC was 1.55 more than G allele carriers (OR=1.55, 95% CI: 0.856-2.828).
Moreover, physical activity in cases was significantly less than controls (P=0.001).
We further observed that the subjects in the case group used fewer non-steroidal antiinflammatory
drugs compared to healthy controls (P=0.02). Analysis of body mass
index (BMI) between cases and controls revealed that the average of BMI in cases
was higher than the controls (P =0.04).
Conclusion: Our results showed that individuals carrying the AA genotype ran a
higher risk of developing CRC compared to GG genotype. |
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| ISSN: | 2008-6709 2008-6687 |