The Association between Cyclin D1 (CCND1) rs9344 AA Genotype and Increased Risk of Colorectal Cancer in An Iranian Population
Background: Colorectal cancer (CRC) is a globally growing disease with a steady decrease in the age of incidence. Pathogenesis of this cancer stems from a complex interaction between environmental factors and genetic predisposition. Among genetic factors, high activity of cyclin D1gene is promine...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Shiraz University of Medical Sciences
2020-07-01
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| Series: | Middle East Journal of Cancer |
| Subjects: | |
| Online Access: | https://mejc.sums.ac.ir/article_46727.html |
| _version_ | 1819055195251802112 |
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| author | Shima Rahimirad Meysam Mosallaei Rasoul Salehi Shervin Shariatpanahi Ahmad Reza Salehi |
| author_facet | Shima Rahimirad Meysam Mosallaei Rasoul Salehi Shervin Shariatpanahi Ahmad Reza Salehi |
| author_sort | Shima Rahimirad |
| collection | DOAJ |
| description | Background: Colorectal cancer (CRC) is a globally growing disease with a steady
decrease in the age of incidence. Pathogenesis of this cancer stems from a complex
interaction between environmental factors and genetic predisposition. Among genetic
factors, high activity of cyclin D1gene is prominent. A polymorphism (G870A) in
exon 4 of cyclin D1 is responsible for a variant transcript with longer half-life and
may culminate in uncontrollable cellular growth, thereby contributing to cancer
development.
Method: This case-control study evaluated the frequency of CCND1 G870A
polymorphism and risk of sporadic CRC in an Iranian population. The study population
comprised 50 CRC patients and 50 CRC-free controls selected on the basis of
colonoscopy examination. For genotyping, we performed polymerase chain reaction
– restriction fragment length polymorphism analysis (PCR-RFLP).
Result: AA genotype frequencies compared to GA+GG genotype frequencies
between cases and controls showed that AA genotype frequency in the case group
was significantly higher than the control group (AA vs. GG + GA: OR= 2.25, 95%
CI: 1.13-5.54, P=0.04). Allele A frequency was 57% in patients and 46% in healthy
subjects. Statistical analysis showed that the odds ratio of carriers with allele A for
risk of CRC was 1.55 more than G allele carriers (OR=1.55, 95% CI: 0.856-2.828).
Moreover, physical activity in cases was significantly less than controls (P=0.001).
We further observed that the subjects in the case group used fewer non-steroidal antiinflammatory
drugs compared to healthy controls (P=0.02). Analysis of body mass
index (BMI) between cases and controls revealed that the average of BMI in cases
was higher than the controls (P =0.04).
Conclusion: Our results showed that individuals carrying the AA genotype ran a
higher risk of developing CRC compared to GG genotype. |
| first_indexed | 2024-12-21T13:03:39Z |
| format | Article |
| id | doaj.art-4e71f253c47141bbb096586421fb8805 |
| institution | Directory Open Access Journal |
| issn | 2008-6709 2008-6687 |
| language | English |
| last_indexed | 2024-12-21T13:03:39Z |
| publishDate | 2020-07-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Middle East Journal of Cancer |
| spelling | doaj.art-4e71f253c47141bbb096586421fb88052022-12-21T19:03:06ZengShiraz University of Medical SciencesMiddle East Journal of Cancer2008-67092008-66872020-07-0111336036410.30476/mejc.2020.81432.1006The Association between Cyclin D1 (CCND1) rs9344 AA Genotype and Increased Risk of Colorectal Cancer in An Iranian PopulationShima Rahimirad0Meysam Mosallaei1Rasoul Salehi2Shervin Shariatpanahi3Ahmad Reza Salehi4Faculty of Sciences, University of Shahrekord, Shahrekord, IranDepartment of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranBackground: Colorectal cancer (CRC) is a globally growing disease with a steady decrease in the age of incidence. Pathogenesis of this cancer stems from a complex interaction between environmental factors and genetic predisposition. Among genetic factors, high activity of cyclin D1gene is prominent. A polymorphism (G870A) in exon 4 of cyclin D1 is responsible for a variant transcript with longer half-life and may culminate in uncontrollable cellular growth, thereby contributing to cancer development. Method: This case-control study evaluated the frequency of CCND1 G870A polymorphism and risk of sporadic CRC in an Iranian population. The study population comprised 50 CRC patients and 50 CRC-free controls selected on the basis of colonoscopy examination. For genotyping, we performed polymerase chain reaction – restriction fragment length polymorphism analysis (PCR-RFLP). Result: AA genotype frequencies compared to GA+GG genotype frequencies between cases and controls showed that AA genotype frequency in the case group was significantly higher than the control group (AA vs. GG + GA: OR= 2.25, 95% CI: 1.13-5.54, P=0.04). Allele A frequency was 57% in patients and 46% in healthy subjects. Statistical analysis showed that the odds ratio of carriers with allele A for risk of CRC was 1.55 more than G allele carriers (OR=1.55, 95% CI: 0.856-2.828). Moreover, physical activity in cases was significantly less than controls (P=0.001). We further observed that the subjects in the case group used fewer non-steroidal antiinflammatory drugs compared to healthy controls (P=0.02). Analysis of body mass index (BMI) between cases and controls revealed that the average of BMI in cases was higher than the controls (P =0.04). Conclusion: Our results showed that individuals carrying the AA genotype ran a higher risk of developing CRC compared to GG genotype.https://mejc.sums.ac.ir/article_46727.htmlcolorectal cancersingle nucleotide polymorphismcyclin d1 gene |
| spellingShingle | Shima Rahimirad Meysam Mosallaei Rasoul Salehi Shervin Shariatpanahi Ahmad Reza Salehi The Association between Cyclin D1 (CCND1) rs9344 AA Genotype and Increased Risk of Colorectal Cancer in An Iranian Population Middle East Journal of Cancer colorectal cancer single nucleotide polymorphism cyclin d1 gene |
| title | The Association between Cyclin D1 (CCND1) rs9344 AA Genotype and Increased Risk of Colorectal Cancer in An Iranian Population |
| title_full | The Association between Cyclin D1 (CCND1) rs9344 AA Genotype and Increased Risk of Colorectal Cancer in An Iranian Population |
| title_fullStr | The Association between Cyclin D1 (CCND1) rs9344 AA Genotype and Increased Risk of Colorectal Cancer in An Iranian Population |
| title_full_unstemmed | The Association between Cyclin D1 (CCND1) rs9344 AA Genotype and Increased Risk of Colorectal Cancer in An Iranian Population |
| title_short | The Association between Cyclin D1 (CCND1) rs9344 AA Genotype and Increased Risk of Colorectal Cancer in An Iranian Population |
| title_sort | association between cyclin d1 ccnd1 rs9344 aa genotype and increased risk of colorectal cancer in an iranian population |
| topic | colorectal cancer single nucleotide polymorphism cyclin d1 gene |
| url | https://mejc.sums.ac.ir/article_46727.html |
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