Structural analysis of the Legionella pneumophila Dot/Icm type IV secretion system core complex
Legionella pneumophila is an opportunistic pathogen that causes the potentially fatal pneumonia Legionnaires’ Disease. This infection and subsequent pathology require the Dot/Icm Type IV Secretion System (T4SS) to deliver effector proteins into host cells. Compared to prototypical T4SSs, the Dot/Icm...
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2020-09-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/59530 |
| _version_ | 1818019865423773696 |
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| author | Clarissa L Durie Michael J Sheedlo Jeong Min Chung Brenda G Byrne Min Su Thomas Knight Michele Swanson D Borden Lacy Melanie D Ohi |
| author_facet | Clarissa L Durie Michael J Sheedlo Jeong Min Chung Brenda G Byrne Min Su Thomas Knight Michele Swanson D Borden Lacy Melanie D Ohi |
| author_sort | Clarissa L Durie |
| collection | DOAJ |
| description | Legionella pneumophila is an opportunistic pathogen that causes the potentially fatal pneumonia Legionnaires’ Disease. This infection and subsequent pathology require the Dot/Icm Type IV Secretion System (T4SS) to deliver effector proteins into host cells. Compared to prototypical T4SSs, the Dot/Icm assembly is much larger, containing ~27 different components including a core complex reported to be composed of five proteins: DotC, DotD, DotF, DotG, and DotH. Using single particle cryo-electron microscopy (cryo-EM), we report reconstructions of the core complex of the Dot/Icm T4SS that includes a symmetry mismatch between distinct structural features of the outer membrane cap (OMC) and periplasmic ring (PR). We present models of known core complex proteins, DotC, DotD, and DotH, and two structurally similar proteins within the core complex, DotK and Lpg0657. This analysis reveals the stoichiometry and contact interfaces between the key proteins of the Dot/Icm T4SS core complex and provides a framework for understanding a complex molecular machine. |
| first_indexed | 2024-04-14T07:57:23Z |
| format | Article |
| id | doaj.art-4e727e751c3643ba8cf52314be90d2c1 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-14T07:57:23Z |
| publishDate | 2020-09-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-4e727e751c3643ba8cf52314be90d2c12022-12-22T02:04:59ZengeLife Sciences Publications LtdeLife2050-084X2020-09-01910.7554/eLife.59530Structural analysis of the Legionella pneumophila Dot/Icm type IV secretion system core complexClarissa L Durie0https://orcid.org/0000-0002-4027-4386Michael J Sheedlo1https://orcid.org/0000-0002-3185-1727Jeong Min Chung2https://orcid.org/0000-0002-4285-8764Brenda G Byrne3Min Su4Thomas Knight5Michele Swanson6https://orcid.org/0000-0003-2542-0266D Borden Lacy7https://orcid.org/0000-0003-2273-8121Melanie D Ohi8https://orcid.org/0000-0003-1750-4793Life Sciences Institute, University of Michigan, Ann Arbor, United StatesDepartment of Pathology, Microbiology, and Immunology, Department of Pathology, Vanderbilt University Medical Center, Nashville, United StatesLife Sciences Institute, University of Michigan, Ann Arbor, United StatesDepartment of Microbiology and Immunology, University of Michigan, Ann Arbor, United StatesLife Sciences Institute, University of Michigan, Ann Arbor, United StatesDepartment of Microbiology and Immunology, University of Michigan, Ann Arbor, United StatesDepartment of Microbiology and Immunology, University of Michigan, Ann Arbor, United StatesDepartment of Pathology, Microbiology, and Immunology, Department of Pathology, Vanderbilt University Medical Center, Nashville, United States; The Veterans Affairs Tennessee Valley Healthcare System, Nashville, United States; Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, United StatesLife Sciences Institute, University of Michigan, Ann Arbor, United StatesLegionella pneumophila is an opportunistic pathogen that causes the potentially fatal pneumonia Legionnaires’ Disease. This infection and subsequent pathology require the Dot/Icm Type IV Secretion System (T4SS) to deliver effector proteins into host cells. Compared to prototypical T4SSs, the Dot/Icm assembly is much larger, containing ~27 different components including a core complex reported to be composed of five proteins: DotC, DotD, DotF, DotG, and DotH. Using single particle cryo-electron microscopy (cryo-EM), we report reconstructions of the core complex of the Dot/Icm T4SS that includes a symmetry mismatch between distinct structural features of the outer membrane cap (OMC) and periplasmic ring (PR). We present models of known core complex proteins, DotC, DotD, and DotH, and two structurally similar proteins within the core complex, DotK and Lpg0657. This analysis reveals the stoichiometry and contact interfaces between the key proteins of the Dot/Icm T4SS core complex and provides a framework for understanding a complex molecular machine.https://elifesciences.org/articles/59530Legionella pneumophilaType IV Secretion Systemcryo-EMpathogenesis |
| spellingShingle | Clarissa L Durie Michael J Sheedlo Jeong Min Chung Brenda G Byrne Min Su Thomas Knight Michele Swanson D Borden Lacy Melanie D Ohi Structural analysis of the Legionella pneumophila Dot/Icm type IV secretion system core complex eLife Legionella pneumophila Type IV Secretion System cryo-EM pathogenesis |
| title | Structural analysis of the Legionella pneumophila Dot/Icm type IV secretion system core complex |
| title_full | Structural analysis of the Legionella pneumophila Dot/Icm type IV secretion system core complex |
| title_fullStr | Structural analysis of the Legionella pneumophila Dot/Icm type IV secretion system core complex |
| title_full_unstemmed | Structural analysis of the Legionella pneumophila Dot/Icm type IV secretion system core complex |
| title_short | Structural analysis of the Legionella pneumophila Dot/Icm type IV secretion system core complex |
| title_sort | structural analysis of the legionella pneumophila dot icm type iv secretion system core complex |
| topic | Legionella pneumophila Type IV Secretion System cryo-EM pathogenesis |
| url | https://elifesciences.org/articles/59530 |
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