In vitro evaluation of Aloe saponaria as a potential feed additive to modulate ruminal fermentation and microbial diversity
ABSTRACTSaponin possessed antiprotozoal activity; therefore, it is generally used to mitigate enteric methane (CH4) emission in ruminant nutrition. The objective of this study was to evaluate the effects of Aloe saponaria (AS) supplementation on rumen fermentation, CH4, and microbial diversity in an...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Journal of Applied Animal Research |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/09712119.2023.2165086 |
| _version_ | 1797448324388749312 |
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| author | Hanbeen Kim Byeong-Woo Kim Daekyum Yoo Joonbeom Moon Inhyuk Kwon Yookyung Lee Jakyeom Seo |
| author_facet | Hanbeen Kim Byeong-Woo Kim Daekyum Yoo Joonbeom Moon Inhyuk Kwon Yookyung Lee Jakyeom Seo |
| author_sort | Hanbeen Kim |
| collection | DOAJ |
| description | ABSTRACTSaponin possessed antiprotozoal activity; therefore, it is generally used to mitigate enteric methane (CH4) emission in ruminant nutrition. The objective of this study was to evaluate the effects of Aloe saponaria (AS) supplementation on rumen fermentation, CH4, and microbial diversity in an in vitro experiment over 48 h incubation. Five treatments were used: (1) no additives (CON), 1% and 2% dosage of heat-dried AS (HAS), and freeze-dried AS (FAS), and the experiments were performed three times. After the incubation, volatile fatty acids (VFAs), CH4 proportion in total gas, and microbial diversity were evaluated. Gas production was assessed at 3, 6, 9, 12, 24, 36, and 48 h incubation. FAS addition significantly increased gas production compared to CON except for 6 and 48 h, whereas HAS addition did not significantly affect the gas production at all observed time points. AS addition significantly increased total VFA and the absolute abundances of fungi and Ruminococcus albus regardless of drying method. There was no significant change in the CH4 proportion at 48 h, although the reduction in ciliate protozoa was detected in FAS-treated groups. In conclusion, FAS may be an effective feed additive to improve ruminal fermentation via enhancing feed utilization by rumen microbes. |
| first_indexed | 2024-03-09T14:08:41Z |
| format | Article |
| id | doaj.art-4e74bb0fb2c940b89782469d316dc8e9 |
| institution | Directory Open Access Journal |
| issn | 0971-2119 0974-1844 |
| language | English |
| last_indexed | 2024-03-09T14:08:41Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Applied Animal Research |
| spelling | doaj.art-4e74bb0fb2c940b89782469d316dc8e92023-11-29T18:00:51ZengTaylor & Francis GroupJournal of Applied Animal Research0971-21190974-18442023-12-0151111512210.1080/09712119.2023.2165086In vitro evaluation of Aloe saponaria as a potential feed additive to modulate ruminal fermentation and microbial diversityHanbeen Kim0Byeong-Woo Kim1Daekyum Yoo2Joonbeom Moon3Inhyuk Kwon4Yookyung Lee5Jakyeom Seo6Department of Animal Science, Life and Industry Convergence Research Institute, Pusan National University, Miryang, Republic of KoreaDepartment of Animal Science, Life and Industry Convergence Research Institute, Pusan National University, Miryang, Republic of KoreaDepartment of Animal Science, Life and Industry Convergence Research Institute, Pusan National University, Miryang, Republic of KoreaDepartment of Animal Science, Life and Industry Convergence Research Institute, Pusan National University, Miryang, Republic of KoreaEASY BIO, Inc., Seoul, KoreaAnimal Nutrition & Physiology Team, National Institute of Animal Science, Rural Development Administration, Wanju, KoreaDepartment of Animal Science, Life and Industry Convergence Research Institute, Pusan National University, Miryang, Republic of KoreaABSTRACTSaponin possessed antiprotozoal activity; therefore, it is generally used to mitigate enteric methane (CH4) emission in ruminant nutrition. The objective of this study was to evaluate the effects of Aloe saponaria (AS) supplementation on rumen fermentation, CH4, and microbial diversity in an in vitro experiment over 48 h incubation. Five treatments were used: (1) no additives (CON), 1% and 2% dosage of heat-dried AS (HAS), and freeze-dried AS (FAS), and the experiments were performed three times. After the incubation, volatile fatty acids (VFAs), CH4 proportion in total gas, and microbial diversity were evaluated. Gas production was assessed at 3, 6, 9, 12, 24, 36, and 48 h incubation. FAS addition significantly increased gas production compared to CON except for 6 and 48 h, whereas HAS addition did not significantly affect the gas production at all observed time points. AS addition significantly increased total VFA and the absolute abundances of fungi and Ruminococcus albus regardless of drying method. There was no significant change in the CH4 proportion at 48 h, although the reduction in ciliate protozoa was detected in FAS-treated groups. In conclusion, FAS may be an effective feed additive to improve ruminal fermentation via enhancing feed utilization by rumen microbes.https://www.tandfonline.com/doi/10.1080/09712119.2023.2165086Aloe saponariadrying methodsantiprotozoal efficacymethanerumen microorganism |
| spellingShingle | Hanbeen Kim Byeong-Woo Kim Daekyum Yoo Joonbeom Moon Inhyuk Kwon Yookyung Lee Jakyeom Seo In vitro evaluation of Aloe saponaria as a potential feed additive to modulate ruminal fermentation and microbial diversity Journal of Applied Animal Research Aloe saponaria drying methods antiprotozoal efficacy methane rumen microorganism |
| title | In vitro evaluation of Aloe saponaria as a potential feed additive to modulate ruminal fermentation and microbial diversity |
| title_full | In vitro evaluation of Aloe saponaria as a potential feed additive to modulate ruminal fermentation and microbial diversity |
| title_fullStr | In vitro evaluation of Aloe saponaria as a potential feed additive to modulate ruminal fermentation and microbial diversity |
| title_full_unstemmed | In vitro evaluation of Aloe saponaria as a potential feed additive to modulate ruminal fermentation and microbial diversity |
| title_short | In vitro evaluation of Aloe saponaria as a potential feed additive to modulate ruminal fermentation and microbial diversity |
| title_sort | in vitro evaluation of aloe saponaria as a potential feed additive to modulate ruminal fermentation and microbial diversity |
| topic | Aloe saponaria drying methods antiprotozoal efficacy methane rumen microorganism |
| url | https://www.tandfonline.com/doi/10.1080/09712119.2023.2165086 |
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