| Summary: | An aerobic, oxidase-positive, catalase-negative, non-motile, oval/rod-shaped, and Gram-negative strain RN2-1<sup>T</sup> was isolated from a Korean forest soil sample. Cells grew in a pH range of 6–9 (optimum range: 7–8), in a temperature range of 20–40 °C (optimum range: 30–35 °C), and in the presence of 0–1% (<i>w</i>/<i>v</i>) NaCl. The major polar lipid profile consisted of phosphatidylglycerol, diphosphatidyl glycerol, phosphatidylethanolamine, phosphatidylcholine, and a group of phospholipids, aminolipids, and polar lipids with unidentified structures. The major quinone was ubiquinone 10. The main fatty acids were summed feature 8 (C18:1 ω7c/C18:1 ω6c), C16:0, Cyclo-C17:0, cyclo C19:0 ω8c, and C18:1 2OH. Phylogenetic similarities based on 16S rDNA sequences showed that strain RN2-1<sup>T</sup> grouped in the family <i>Acetobacteraceae</i> included the genera <i>Rhodovastum</i>, <i>Acidisoma</i>, <i>Acidisphaera</i>, and <i>Acidibrevibacterium</i>. The aerobic phototrophic bacterium <i>Rhodovastum atsumiense</i> G2-11<sup>T</sup> was the closest relative to RN2-1<sup>T</sup> (96.04% sequence similarity). Genomic DNA revealed 68.7% G+C content. Whole genome analysis and phenotypic data of the strain RN2-1<sup>T</sup> confirmed its distinctive phylogenetic location within the family <i>Acetobacteraceae</i>. Because of these results, strain RN2-1<sup>T</sup> represents the novel species of a new genus for which the name <i>Limobrevibacterium gyesilva</i> gen. nov., sp. nov. was proposed. The type of strain was strain RN2-1<sup>T</sup> (KACC 23001 = JCM 35944).
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