<i>Limobrevibacterium gyesilva</i> gen. nov., sp. nov., Isolated from Forest Soil
An aerobic, oxidase-positive, catalase-negative, non-motile, oval/rod-shaped, and Gram-negative strain RN2-1<sup>T</sup> was isolated from a Korean forest soil sample. Cells grew in a pH range of 6–9 (optimum range: 7–8), in a temperature range of 20–40 °C (optimum range: 30–35 °C), and...
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MDPI AG
2023-05-01
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| Online Access: | https://www.mdpi.com/1424-2818/15/5/684 |
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| author | Nhan Le Thi Tuyet Jaisoo Kim |
| author_facet | Nhan Le Thi Tuyet Jaisoo Kim |
| author_sort | Nhan Le Thi Tuyet |
| collection | DOAJ |
| description | An aerobic, oxidase-positive, catalase-negative, non-motile, oval/rod-shaped, and Gram-negative strain RN2-1<sup>T</sup> was isolated from a Korean forest soil sample. Cells grew in a pH range of 6–9 (optimum range: 7–8), in a temperature range of 20–40 °C (optimum range: 30–35 °C), and in the presence of 0–1% (<i>w</i>/<i>v</i>) NaCl. The major polar lipid profile consisted of phosphatidylglycerol, diphosphatidyl glycerol, phosphatidylethanolamine, phosphatidylcholine, and a group of phospholipids, aminolipids, and polar lipids with unidentified structures. The major quinone was ubiquinone 10. The main fatty acids were summed feature 8 (C18:1 ω7c/C18:1 ω6c), C16:0, Cyclo-C17:0, cyclo C19:0 ω8c, and C18:1 2OH. Phylogenetic similarities based on 16S rDNA sequences showed that strain RN2-1<sup>T</sup> grouped in the family <i>Acetobacteraceae</i> included the genera <i>Rhodovastum</i>, <i>Acidisoma</i>, <i>Acidisphaera</i>, and <i>Acidibrevibacterium</i>. The aerobic phototrophic bacterium <i>Rhodovastum atsumiense</i> G2-11<sup>T</sup> was the closest relative to RN2-1<sup>T</sup> (96.04% sequence similarity). Genomic DNA revealed 68.7% G+C content. Whole genome analysis and phenotypic data of the strain RN2-1<sup>T</sup> confirmed its distinctive phylogenetic location within the family <i>Acetobacteraceae</i>. Because of these results, strain RN2-1<sup>T</sup> represents the novel species of a new genus for which the name <i>Limobrevibacterium gyesilva</i> gen. nov., sp. nov. was proposed. The type of strain was strain RN2-1<sup>T</sup> (KACC 23001 = JCM 35944). |
| first_indexed | 2024-03-11T03:47:34Z |
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| institution | Directory Open Access Journal |
| issn | 1424-2818 |
| language | English |
| last_indexed | 2024-03-11T03:47:34Z |
| publishDate | 2023-05-01 |
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| spelling | doaj.art-4e74d4dd251448088894ffab863106062023-11-18T01:06:35ZengMDPI AGDiversity1424-28182023-05-0115568410.3390/d15050684<i>Limobrevibacterium gyesilva</i> gen. nov., sp. nov., Isolated from Forest SoilNhan Le Thi Tuyet0Jaisoo Kim1Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon 16227, Gyeonggi-do, Republic of KoreaDepartment of Life Science, College of Natural Sciences, Kyonggi University, Suwon 16227, Gyeonggi-do, Republic of KoreaAn aerobic, oxidase-positive, catalase-negative, non-motile, oval/rod-shaped, and Gram-negative strain RN2-1<sup>T</sup> was isolated from a Korean forest soil sample. Cells grew in a pH range of 6–9 (optimum range: 7–8), in a temperature range of 20–40 °C (optimum range: 30–35 °C), and in the presence of 0–1% (<i>w</i>/<i>v</i>) NaCl. The major polar lipid profile consisted of phosphatidylglycerol, diphosphatidyl glycerol, phosphatidylethanolamine, phosphatidylcholine, and a group of phospholipids, aminolipids, and polar lipids with unidentified structures. The major quinone was ubiquinone 10. The main fatty acids were summed feature 8 (C18:1 ω7c/C18:1 ω6c), C16:0, Cyclo-C17:0, cyclo C19:0 ω8c, and C18:1 2OH. Phylogenetic similarities based on 16S rDNA sequences showed that strain RN2-1<sup>T</sup> grouped in the family <i>Acetobacteraceae</i> included the genera <i>Rhodovastum</i>, <i>Acidisoma</i>, <i>Acidisphaera</i>, and <i>Acidibrevibacterium</i>. The aerobic phototrophic bacterium <i>Rhodovastum atsumiense</i> G2-11<sup>T</sup> was the closest relative to RN2-1<sup>T</sup> (96.04% sequence similarity). Genomic DNA revealed 68.7% G+C content. Whole genome analysis and phenotypic data of the strain RN2-1<sup>T</sup> confirmed its distinctive phylogenetic location within the family <i>Acetobacteraceae</i>. Because of these results, strain RN2-1<sup>T</sup> represents the novel species of a new genus for which the name <i>Limobrevibacterium gyesilva</i> gen. nov., sp. nov. was proposed. The type of strain was strain RN2-1<sup>T</sup> (KACC 23001 = JCM 35944).https://www.mdpi.com/1424-2818/15/5/684<i>Limobrevibacterium gyesilva</i>forest soilnovel genus<i>Acetobacteraceae</i> |
| spellingShingle | Nhan Le Thi Tuyet Jaisoo Kim <i>Limobrevibacterium gyesilva</i> gen. nov., sp. nov., Isolated from Forest Soil Diversity <i>Limobrevibacterium gyesilva</i> forest soil novel genus <i>Acetobacteraceae</i> |
| title | <i>Limobrevibacterium gyesilva</i> gen. nov., sp. nov., Isolated from Forest Soil |
| title_full | <i>Limobrevibacterium gyesilva</i> gen. nov., sp. nov., Isolated from Forest Soil |
| title_fullStr | <i>Limobrevibacterium gyesilva</i> gen. nov., sp. nov., Isolated from Forest Soil |
| title_full_unstemmed | <i>Limobrevibacterium gyesilva</i> gen. nov., sp. nov., Isolated from Forest Soil |
| title_short | <i>Limobrevibacterium gyesilva</i> gen. nov., sp. nov., Isolated from Forest Soil |
| title_sort | i limobrevibacterium gyesilva i gen nov sp nov isolated from forest soil |
| topic | <i>Limobrevibacterium gyesilva</i> forest soil novel genus <i>Acetobacteraceae</i> |
| url | https://www.mdpi.com/1424-2818/15/5/684 |
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