Proteomic changes between male and female worms of the polychaetous annelid Neanthes arenaceodentata before and after spawning.
The Neanthesacuminata species complex (Polychaeta) are cosmopolitan in distribution. Neanthesarenaceodentata, Southern California member of the N. acuminata complex, has been widely used as toxicological test animal in the marine environment. Method of reproduction is unique in this polychaete compl...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3758283?pdf=render |
| _version_ | 1818326849115127808 |
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| author | Kondethimmanahalli H Chandramouli Timothy Ravasi Donald Reish Pei-Yuan Qian |
| author_facet | Kondethimmanahalli H Chandramouli Timothy Ravasi Donald Reish Pei-Yuan Qian |
| author_sort | Kondethimmanahalli H Chandramouli |
| collection | DOAJ |
| description | The Neanthesacuminata species complex (Polychaeta) are cosmopolitan in distribution. Neanthesarenaceodentata, Southern California member of the N. acuminata complex, has been widely used as toxicological test animal in the marine environment. Method of reproduction is unique in this polychaete complex. Same sexes fight and opposite sexes lie side by side until egg laying. Females lose about 75% of their weight and die after laying eggs. The male, capable of reproducing up to nine times, fertilizes the eggs and incubates the embryos for 3-4 weeks. The objective of this study was to determine if there is any set of proteins that influences this unique pattern of reproduction. Gel-based two-dimensional electrophoresis (2-DE) and gel-free quantitative proteomics methods were used to identify differential protein expression patterns before and after spawning in both male and female N. arenaceodentata. Males showed a higher degree of similarity in protein expression patterns but females showed large changes in phosphoproteme before and after spawning. There was a decrease (about 70%) in the number of detected phosphoproteins in spent females. The proteins involved in muscular development, cell signaling, structure and integrity, and translation were differentially expressed. This study provides proteomic insights of the male and female worms that may serve as a foundation for better understanding of unusual reproductive patterns in polychaete worms. |
| first_indexed | 2024-12-13T12:06:54Z |
| format | Article |
| id | doaj.art-4e7699174fbc4c6586a6c78f18bf3473 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T12:06:54Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-4e7699174fbc4c6586a6c78f18bf34732022-12-21T23:46:55ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0188e7299010.1371/journal.pone.0072990Proteomic changes between male and female worms of the polychaetous annelid Neanthes arenaceodentata before and after spawning.Kondethimmanahalli H ChandramouliTimothy RavasiDonald ReishPei-Yuan QianThe Neanthesacuminata species complex (Polychaeta) are cosmopolitan in distribution. Neanthesarenaceodentata, Southern California member of the N. acuminata complex, has been widely used as toxicological test animal in the marine environment. Method of reproduction is unique in this polychaete complex. Same sexes fight and opposite sexes lie side by side until egg laying. Females lose about 75% of their weight and die after laying eggs. The male, capable of reproducing up to nine times, fertilizes the eggs and incubates the embryos for 3-4 weeks. The objective of this study was to determine if there is any set of proteins that influences this unique pattern of reproduction. Gel-based two-dimensional electrophoresis (2-DE) and gel-free quantitative proteomics methods were used to identify differential protein expression patterns before and after spawning in both male and female N. arenaceodentata. Males showed a higher degree of similarity in protein expression patterns but females showed large changes in phosphoproteme before and after spawning. There was a decrease (about 70%) in the number of detected phosphoproteins in spent females. The proteins involved in muscular development, cell signaling, structure and integrity, and translation were differentially expressed. This study provides proteomic insights of the male and female worms that may serve as a foundation for better understanding of unusual reproductive patterns in polychaete worms.http://europepmc.org/articles/PMC3758283?pdf=render |
| spellingShingle | Kondethimmanahalli H Chandramouli Timothy Ravasi Donald Reish Pei-Yuan Qian Proteomic changes between male and female worms of the polychaetous annelid Neanthes arenaceodentata before and after spawning. PLoS ONE |
| title | Proteomic changes between male and female worms of the polychaetous annelid Neanthes arenaceodentata before and after spawning. |
| title_full | Proteomic changes between male and female worms of the polychaetous annelid Neanthes arenaceodentata before and after spawning. |
| title_fullStr | Proteomic changes between male and female worms of the polychaetous annelid Neanthes arenaceodentata before and after spawning. |
| title_full_unstemmed | Proteomic changes between male and female worms of the polychaetous annelid Neanthes arenaceodentata before and after spawning. |
| title_short | Proteomic changes between male and female worms of the polychaetous annelid Neanthes arenaceodentata before and after spawning. |
| title_sort | proteomic changes between male and female worms of the polychaetous annelid neanthes arenaceodentata before and after spawning |
| url | http://europepmc.org/articles/PMC3758283?pdf=render |
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