Neuroprotective effects of zonisamide on cerebral ischemia injury via inhibition of neuronal apoptosis
It is known that neuronal apoptosis contributes to pathology of cerebral ischemia injury. Zonisamide (ZNS) has shown anti-apoptosis effects in recent studies. The present study investigated whether the anti-apoptotic effect can account for the neuroprotective action of ZNS on cerebral ischemia. Neur...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2021-02-01
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| Series: | Brazilian Journal of Medical and Biological Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021000400608&tlng=en |
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| author | Junna He Xiangjian Zhang Weiliang He Yanzhao Xie Yanxia Chen Yang Yang Rong Chen |
| author_facet | Junna He Xiangjian Zhang Weiliang He Yanzhao Xie Yanxia Chen Yang Yang Rong Chen |
| author_sort | Junna He |
| collection | DOAJ |
| description | It is known that neuronal apoptosis contributes to pathology of cerebral ischemia injury. Zonisamide (ZNS) has shown anti-apoptosis effects in recent studies. The present study investigated whether the anti-apoptotic effect can account for the neuroprotective action of ZNS on cerebral ischemia. Neuronal cells were maintained under oxygen-glucose deprivation conditions to simulate cerebral ischemia and treated with ZNS simultaneously. The apoptosis of the cells and expression of apoptosis-related proteins were investigated by flow cytometry and western blot analysis, respectively. A cerebral ischemia mouse model was created via middle cerebral artery occlusion, and the mice were treated with ZNS. Neurological deficit scores and infarct volumes of the cerebral ischemia mice were measured. The apoptosis status of the neuronal cells was evaluated by TUNEL staining. In vitro, the ZNS treatment inhibited both the apoptosis of the neuronal cells and apoptosis-related protein expression (caspase-3, caspase-8, and calpain-1) induced by the oxygen-glucose deprivation. The anti-apoptosis effect of ZNS could occur through the blocking of reactive oxygen species. Moreover, ZNS treatment significantly ameliorated neurological deficits and reduced infarct volumes in the cerebral ischemia mice model. In this study, ZNS exerted neuroprotective effects by inhibition of apoptosis in neuronal cells in cerebral ischemia. Therefore, ZNS might be a promising therapy for cerebral ischemia. |
| first_indexed | 2024-12-18T02:42:59Z |
| format | Article |
| id | doaj.art-4e785331555d46a58c8cb320968208a5 |
| institution | Directory Open Access Journal |
| issn | 1414-431X |
| language | English |
| last_indexed | 2024-12-18T02:42:59Z |
| publishDate | 2021-02-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-4e785331555d46a58c8cb320968208a52022-12-21T21:23:36ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research1414-431X2021-02-0154410.1590/1414-431x202010498Neuroprotective effects of zonisamide on cerebral ischemia injury via inhibition of neuronal apoptosisJunna Hehttps://orcid.org/0000-0001-6792-4548Xiangjian Zhanghttps://orcid.org/0000-0002-7543-030XWeiliang Hehttps://orcid.org/0000-0002-4703-1336Yanzhao Xiehttps://orcid.org/0000-0001-7394-561XYanxia Chenhttps://orcid.org/0000-0001-8713-5044Yang Yanghttps://orcid.org/0000-0002-4750-4156Rong Chenhttps://orcid.org/0000-0002-1794-1777It is known that neuronal apoptosis contributes to pathology of cerebral ischemia injury. Zonisamide (ZNS) has shown anti-apoptosis effects in recent studies. The present study investigated whether the anti-apoptotic effect can account for the neuroprotective action of ZNS on cerebral ischemia. Neuronal cells were maintained under oxygen-glucose deprivation conditions to simulate cerebral ischemia and treated with ZNS simultaneously. The apoptosis of the cells and expression of apoptosis-related proteins were investigated by flow cytometry and western blot analysis, respectively. A cerebral ischemia mouse model was created via middle cerebral artery occlusion, and the mice were treated with ZNS. Neurological deficit scores and infarct volumes of the cerebral ischemia mice were measured. The apoptosis status of the neuronal cells was evaluated by TUNEL staining. In vitro, the ZNS treatment inhibited both the apoptosis of the neuronal cells and apoptosis-related protein expression (caspase-3, caspase-8, and calpain-1) induced by the oxygen-glucose deprivation. The anti-apoptosis effect of ZNS could occur through the blocking of reactive oxygen species. Moreover, ZNS treatment significantly ameliorated neurological deficits and reduced infarct volumes in the cerebral ischemia mice model. In this study, ZNS exerted neuroprotective effects by inhibition of apoptosis in neuronal cells in cerebral ischemia. Therefore, ZNS might be a promising therapy for cerebral ischemia.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021000400608&tlng=enCerebral ischemiaZonisamideNeuroprotectionApoptosisNeuronal cells |
| spellingShingle | Junna He Xiangjian Zhang Weiliang He Yanzhao Xie Yanxia Chen Yang Yang Rong Chen Neuroprotective effects of zonisamide on cerebral ischemia injury via inhibition of neuronal apoptosis Brazilian Journal of Medical and Biological Research Cerebral ischemia Zonisamide Neuroprotection Apoptosis Neuronal cells |
| title | Neuroprotective effects of zonisamide on cerebral ischemia injury via inhibition of neuronal apoptosis |
| title_full | Neuroprotective effects of zonisamide on cerebral ischemia injury via inhibition of neuronal apoptosis |
| title_fullStr | Neuroprotective effects of zonisamide on cerebral ischemia injury via inhibition of neuronal apoptosis |
| title_full_unstemmed | Neuroprotective effects of zonisamide on cerebral ischemia injury via inhibition of neuronal apoptosis |
| title_short | Neuroprotective effects of zonisamide on cerebral ischemia injury via inhibition of neuronal apoptosis |
| title_sort | neuroprotective effects of zonisamide on cerebral ischemia injury via inhibition of neuronal apoptosis |
| topic | Cerebral ischemia Zonisamide Neuroprotection Apoptosis Neuronal cells |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021000400608&tlng=en |
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