Isolation of Mycoplasma spp. from Geese with Pneumonia and Identification of Microbial Isolates via Molecular Methods
ABSTRACT This study aimed to investigate Mycoplasma species in the lungs of 500 geese with pneumonia from the Kars region (Turkey) via cultural and molecular methods. The samples were cultured on Frey’s Broth and Agar media. To identify Mycoplasma species a Growth Inhibition Test was used. The ident...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Fundação APINCO de Ciência e Tecnologia Avícolas
2022-02-01
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| Series: | Brazilian Journal of Poultry Science |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2022000100304&lng=en&tlng=en |
| _version_ | 1818296265979461632 |
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| author | Ö Çelebi Ü Özdemir F Büyük A Ünsal Baca ŞH Erpek M Karahan S Otlu M Şahin MR Coşkun E Çelik A Gülmez Sağlam E Büyük D Akça |
| author_facet | Ö Çelebi Ü Özdemir F Büyük A Ünsal Baca ŞH Erpek M Karahan S Otlu M Şahin MR Coşkun E Çelik A Gülmez Sağlam E Büyük D Akça |
| author_sort | Ö Çelebi |
| collection | DOAJ |
| description | ABSTRACT This study aimed to investigate Mycoplasma species in the lungs of 500 geese with pneumonia from the Kars region (Turkey) via cultural and molecular methods. The samples were cultured on Frey’s Broth and Agar media. To identify Mycoplasma species a Growth Inhibition Test was used. The identification was continued with species-specific PCR and sequence analysis which provide amplification of the genes dnaX, pcrA, rpoB, and the sequence of the 16S rRNA gene, respectively. In addition, Mycoplasma gallisepticum and Mycoplasma synoviae from pneumonic lung samples were directly analyzed via Multiplex Real-time PCR. As a result, 51 Mycoplasma strains were isolated and 32 were identified as Mycoplasma anatis, 9 as Mycoplasma anseris, 5 as Mycoplasma cloacale and 3 as Mycoplasma anserisalpingitis. Two Mycoplasma isolates that could not be identified were grouped in the same branch as a result of 16S RNA sequencing and their nearest neighbour was found to be Mycoplasma sp. 2045 (GenBankNo.MK615061.1). M. gallisepticum DNA was detected in 3 pneumonic lung samples and M. gallisepticum/M. synoviae DNAs were found simultaneously in 1 sample. While some Mycoplasma species identified in this study consolidated their place as pneumonic agents, some increased their potential to become a pneumonic agent when compared with cases caused by well-recognized Mycoplasma strains. Two isolates were identified as -Mycoplasma spp. as their 16S rRNA gene sequence identity levels scored below the threshold of 98.7% for species demarcation and still need to be defined whether they are possible representatives of a novel Mycoplasma species. |
| first_indexed | 2024-12-13T04:00:48Z |
| format | Article |
| id | doaj.art-4e78ca5584a841e3a237527f96728f98 |
| institution | Directory Open Access Journal |
| issn | 1806-9061 |
| language | English |
| last_indexed | 2024-12-13T04:00:48Z |
| publishDate | 2022-02-01 |
| publisher | Fundação APINCO de Ciência e Tecnologia Avícolas |
| record_format | Article |
| series | Brazilian Journal of Poultry Science |
| spelling | doaj.art-4e78ca5584a841e3a237527f96728f982022-12-22T00:00:27ZengFundação APINCO de Ciência e Tecnologia AvícolasBrazilian Journal of Poultry Science1806-90612022-02-0124110.1590/1806-9061-2021-1522Isolation of Mycoplasma spp. from Geese with Pneumonia and Identification of Microbial Isolates via Molecular MethodsÖ Çelebihttps://orcid.org/0000-0002-3478-008XÜ Özdemirhttps://orcid.org/0000-0002-1504-4685F Büyükhttps://orcid.org/0000-0003-3278-4834A Ünsal Bacahttps://orcid.org/0000-0002-6500-151XŞH Erpekhttps://orcid.org/0000-0002-7266-0847M Karahanhttps://orcid.org/0000-0001-7704-7630S Otluhttps://orcid.org/0000-0001-8490-2279M Şahinhttps://orcid.org/0000-0003-0106-5677MR Coşkunhttps://orcid.org/0000-0002-1441-3995E Çelikhttps://orcid.org/0000-0003-4531-3863A Gülmez Sağlamhttps://orcid.org/0000-0002-7639-5075E Büyükhttps://orcid.org/0000-0003-2071-8901D Akçahttps://orcid.org/0000-0002-3986-8769ABSTRACT This study aimed to investigate Mycoplasma species in the lungs of 500 geese with pneumonia from the Kars region (Turkey) via cultural and molecular methods. The samples were cultured on Frey’s Broth and Agar media. To identify Mycoplasma species a Growth Inhibition Test was used. The identification was continued with species-specific PCR and sequence analysis which provide amplification of the genes dnaX, pcrA, rpoB, and the sequence of the 16S rRNA gene, respectively. In addition, Mycoplasma gallisepticum and Mycoplasma synoviae from pneumonic lung samples were directly analyzed via Multiplex Real-time PCR. As a result, 51 Mycoplasma strains were isolated and 32 were identified as Mycoplasma anatis, 9 as Mycoplasma anseris, 5 as Mycoplasma cloacale and 3 as Mycoplasma anserisalpingitis. Two Mycoplasma isolates that could not be identified were grouped in the same branch as a result of 16S RNA sequencing and their nearest neighbour was found to be Mycoplasma sp. 2045 (GenBankNo.MK615061.1). M. gallisepticum DNA was detected in 3 pneumonic lung samples and M. gallisepticum/M. synoviae DNAs were found simultaneously in 1 sample. While some Mycoplasma species identified in this study consolidated their place as pneumonic agents, some increased their potential to become a pneumonic agent when compared with cases caused by well-recognized Mycoplasma strains. Two isolates were identified as -Mycoplasma spp. as their 16S rRNA gene sequence identity levels scored below the threshold of 98.7% for species demarcation and still need to be defined whether they are possible representatives of a novel Mycoplasma species.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2022000100304&lng=en&tlng=enCulturegooseMycoplasma sppPCR16S rRNA sequencing |
| spellingShingle | Ö Çelebi Ü Özdemir F Büyük A Ünsal Baca ŞH Erpek M Karahan S Otlu M Şahin MR Coşkun E Çelik A Gülmez Sağlam E Büyük D Akça Isolation of Mycoplasma spp. from Geese with Pneumonia and Identification of Microbial Isolates via Molecular Methods Brazilian Journal of Poultry Science Culture goose Mycoplasma spp PCR 16S rRNA sequencing |
| title | Isolation of Mycoplasma spp. from Geese with Pneumonia and Identification of Microbial Isolates via Molecular Methods |
| title_full | Isolation of Mycoplasma spp. from Geese with Pneumonia and Identification of Microbial Isolates via Molecular Methods |
| title_fullStr | Isolation of Mycoplasma spp. from Geese with Pneumonia and Identification of Microbial Isolates via Molecular Methods |
| title_full_unstemmed | Isolation of Mycoplasma spp. from Geese with Pneumonia and Identification of Microbial Isolates via Molecular Methods |
| title_short | Isolation of Mycoplasma spp. from Geese with Pneumonia and Identification of Microbial Isolates via Molecular Methods |
| title_sort | isolation of mycoplasma spp from geese with pneumonia and identification of microbial isolates via molecular methods |
| topic | Culture goose Mycoplasma spp PCR 16S rRNA sequencing |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2022000100304&lng=en&tlng=en |
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