Mycobacteria-Specific T Cells Are Generated in the Lung During Mucosal BCG Immunization or Infection With Mycobacterium tuberculosis
Specific T cell responses are central for protection against infection with M. tuberculosis. Here we show that mycobacteria-specific CD4 and CD8 T cells accumulated in the lung but not in the mediastinal lymph node (MLN) at different time points after M. tuberculosis infection or BCG immunization. P...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2020-10-01
|
| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2020.566319/full |
| _version_ | 1818972648284094464 |
|---|---|
| author | Juan I. Basile Ruining Liu Wenjun Mou Yu Gao Berit Carow Martin E. Rottenberg |
| author_facet | Juan I. Basile Ruining Liu Wenjun Mou Yu Gao Berit Carow Martin E. Rottenberg |
| author_sort | Juan I. Basile |
| collection | DOAJ |
| description | Specific T cell responses are central for protection against infection with M. tuberculosis. Here we show that mycobacteria-specific CD4 and CD8 T cells accumulated in the lung but not in the mediastinal lymph node (MLN) at different time points after M. tuberculosis infection or BCG immunization. Proliferating specific T cells were found in the lung after infection and immunization. Pulmonary, but not MLN-derived CD4 and CD8 T cells, from M. tuberculosis-infected mice secreted IFN-γ after stimulation with different mycobacterial peptides. Mycobacteria-specific resident memory CD4 and CD8 T cells (TRM) expressing PD-1 accumulated in the lung after aerosol infection and intratracheal (i.t.) -but not subcutaneous (s.c.)- BCG immunization. Chemical inhibition of recirculation indicated that TRM were generated in the lung after BCG i.t. immunization. In summary, mycobacteria specific-TRM accumulate in the lung during i.t. but not s.c. immunization or M. tuberculosis infection. Collectively our data suggests that priming, accumulation and/or expansion of specific T cells during BCG immunization and M. tuberculosis infection occurs in the lung. |
| first_indexed | 2024-12-20T15:11:36Z |
| format | Article |
| id | doaj.art-4e79558d433245a2b4ee3a3fb3b0f516 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-20T15:11:36Z |
| publishDate | 2020-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-4e79558d433245a2b4ee3a3fb3b0f5162022-12-21T19:36:18ZengFrontiers Media S.A.Frontiers in Immunology1664-32242020-10-011110.3389/fimmu.2020.566319566319Mycobacteria-Specific T Cells Are Generated in the Lung During Mucosal BCG Immunization or Infection With Mycobacterium tuberculosisJuan I. BasileRuining LiuWenjun MouYu GaoBerit CarowMartin E. RottenbergSpecific T cell responses are central for protection against infection with M. tuberculosis. Here we show that mycobacteria-specific CD4 and CD8 T cells accumulated in the lung but not in the mediastinal lymph node (MLN) at different time points after M. tuberculosis infection or BCG immunization. Proliferating specific T cells were found in the lung after infection and immunization. Pulmonary, but not MLN-derived CD4 and CD8 T cells, from M. tuberculosis-infected mice secreted IFN-γ after stimulation with different mycobacterial peptides. Mycobacteria-specific resident memory CD4 and CD8 T cells (TRM) expressing PD-1 accumulated in the lung after aerosol infection and intratracheal (i.t.) -but not subcutaneous (s.c.)- BCG immunization. Chemical inhibition of recirculation indicated that TRM were generated in the lung after BCG i.t. immunization. In summary, mycobacteria specific-TRM accumulate in the lung during i.t. but not s.c. immunization or M. tuberculosis infection. Collectively our data suggests that priming, accumulation and/or expansion of specific T cells during BCG immunization and M. tuberculosis infection occurs in the lung.https://www.frontiersin.org/articles/10.3389/fimmu.2020.566319/fullMycobacterium tuberculosisT celllungresident memory T cellsBCG- immunization |
| spellingShingle | Juan I. Basile Ruining Liu Wenjun Mou Yu Gao Berit Carow Martin E. Rottenberg Mycobacteria-Specific T Cells Are Generated in the Lung During Mucosal BCG Immunization or Infection With Mycobacterium tuberculosis Frontiers in Immunology Mycobacterium tuberculosis T cell lung resident memory T cells BCG- immunization |
| title | Mycobacteria-Specific T Cells Are Generated in the Lung During Mucosal BCG Immunization or Infection With Mycobacterium tuberculosis |
| title_full | Mycobacteria-Specific T Cells Are Generated in the Lung During Mucosal BCG Immunization or Infection With Mycobacterium tuberculosis |
| title_fullStr | Mycobacteria-Specific T Cells Are Generated in the Lung During Mucosal BCG Immunization or Infection With Mycobacterium tuberculosis |
| title_full_unstemmed | Mycobacteria-Specific T Cells Are Generated in the Lung During Mucosal BCG Immunization or Infection With Mycobacterium tuberculosis |
| title_short | Mycobacteria-Specific T Cells Are Generated in the Lung During Mucosal BCG Immunization or Infection With Mycobacterium tuberculosis |
| title_sort | mycobacteria specific t cells are generated in the lung during mucosal bcg immunization or infection with mycobacterium tuberculosis |
| topic | Mycobacterium tuberculosis T cell lung resident memory T cells BCG- immunization |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2020.566319/full |
| work_keys_str_mv | AT juanibasile mycobacteriaspecifictcellsaregeneratedinthelungduringmucosalbcgimmunizationorinfectionwithmycobacteriumtuberculosis AT ruiningliu mycobacteriaspecifictcellsaregeneratedinthelungduringmucosalbcgimmunizationorinfectionwithmycobacteriumtuberculosis AT wenjunmou mycobacteriaspecifictcellsaregeneratedinthelungduringmucosalbcgimmunizationorinfectionwithmycobacteriumtuberculosis AT yugao mycobacteriaspecifictcellsaregeneratedinthelungduringmucosalbcgimmunizationorinfectionwithmycobacteriumtuberculosis AT beritcarow mycobacteriaspecifictcellsaregeneratedinthelungduringmucosalbcgimmunizationorinfectionwithmycobacteriumtuberculosis AT martinerottenberg mycobacteriaspecifictcellsaregeneratedinthelungduringmucosalbcgimmunizationorinfectionwithmycobacteriumtuberculosis |