Molecular detection and identification of three intracellular parasites of retail mutton products in Beijing, China
Sarcocystis spp., Neospora caninum and Toxoplasma gondii are globally ubiquitous pathogens, and domestic sheep are considered to be one of the intermediate hosts. 83 myocardial samples of sheep were collected from 12 retail stores in Beijing, China. Sarcocystis spp., N. caninum and T. gondii were id...
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2022.1018788/full |
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| author | Zifu Zhu Yajie Chen Xu Yang Lifang Wang Qun Liu Qun Liu Jing Liu Jing Liu |
| author_facet | Zifu Zhu Yajie Chen Xu Yang Lifang Wang Qun Liu Qun Liu Jing Liu Jing Liu |
| author_sort | Zifu Zhu |
| collection | DOAJ |
| description | Sarcocystis spp., Neospora caninum and Toxoplasma gondii are globally ubiquitous pathogens, and domestic sheep are considered to be one of the intermediate hosts. 83 myocardial samples of sheep were collected from 12 retail stores in Beijing, China. Sarcocystis spp., N. caninum and T. gondii were identified by PCR amplification of the 18S rRNA gene, Nc-5 gene and 529bp DNA fragment with a prevalence of 86.7% (95% CI: 77.5–93.2) and 43.4% (95% CI: 32.5–54.7) for Sarcocystis spp. and N. caninum infections, respectively, and no T. gondii was detected. The co-infection prevalence of Sarcocystis and N. caninum was 38.6% (95% CI: 28.1–49.9). Two Sarcocystis species were subtyped by analyzing 18SrRNA sequences and were identified as Sarcocystis tenella and Sarcocystis arieticanis. The prevalence of S. tenella and S. arieticanis infections was 84.3% (95% CI: 74.7–91.4) and 56.6% (95% CI: 45.3–67.5), respectively. This study shows that sheep have a high risk of infection with Sarcocystis and N. caninum, suggests that effective prevention measures are needed to avoid the spread of these parasites in sheep. Toxoplasmosis in sheep poses a threat to human and animal health and requires monitoring and preventing continuously. |
| first_indexed | 2024-04-12T16:59:40Z |
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| id | doaj.art-4e7b25a971634b2f862883761a87fa76 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-04-12T16:59:40Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-4e7b25a971634b2f862883761a87fa762022-12-22T03:24:07ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692022-09-01910.3389/fvets.2022.10187881018788Molecular detection and identification of three intracellular parasites of retail mutton products in Beijing, ChinaZifu Zhu0Yajie Chen1Xu Yang2Lifang Wang3Qun Liu4Qun Liu5Jing Liu6Jing Liu7National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, ChinaNational Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, ChinaNational Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, ChinaNational Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, ChinaNational Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, ChinaKey Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, ChinaNational Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, ChinaKey Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, ChinaSarcocystis spp., Neospora caninum and Toxoplasma gondii are globally ubiquitous pathogens, and domestic sheep are considered to be one of the intermediate hosts. 83 myocardial samples of sheep were collected from 12 retail stores in Beijing, China. Sarcocystis spp., N. caninum and T. gondii were identified by PCR amplification of the 18S rRNA gene, Nc-5 gene and 529bp DNA fragment with a prevalence of 86.7% (95% CI: 77.5–93.2) and 43.4% (95% CI: 32.5–54.7) for Sarcocystis spp. and N. caninum infections, respectively, and no T. gondii was detected. The co-infection prevalence of Sarcocystis and N. caninum was 38.6% (95% CI: 28.1–49.9). Two Sarcocystis species were subtyped by analyzing 18SrRNA sequences and were identified as Sarcocystis tenella and Sarcocystis arieticanis. The prevalence of S. tenella and S. arieticanis infections was 84.3% (95% CI: 74.7–91.4) and 56.6% (95% CI: 45.3–67.5), respectively. This study shows that sheep have a high risk of infection with Sarcocystis and N. caninum, suggests that effective prevention measures are needed to avoid the spread of these parasites in sheep. Toxoplasmosis in sheep poses a threat to human and animal health and requires monitoring and preventing continuously.https://www.frontiersin.org/articles/10.3389/fvets.2022.1018788/fullsheepSarcocystis spp.N. caninumT. gondiiprevalence |
| spellingShingle | Zifu Zhu Yajie Chen Xu Yang Lifang Wang Qun Liu Qun Liu Jing Liu Jing Liu Molecular detection and identification of three intracellular parasites of retail mutton products in Beijing, China Frontiers in Veterinary Science sheep Sarcocystis spp. N. caninum T. gondii prevalence |
| title | Molecular detection and identification of three intracellular parasites of retail mutton products in Beijing, China |
| title_full | Molecular detection and identification of three intracellular parasites of retail mutton products in Beijing, China |
| title_fullStr | Molecular detection and identification of three intracellular parasites of retail mutton products in Beijing, China |
| title_full_unstemmed | Molecular detection and identification of three intracellular parasites of retail mutton products in Beijing, China |
| title_short | Molecular detection and identification of three intracellular parasites of retail mutton products in Beijing, China |
| title_sort | molecular detection and identification of three intracellular parasites of retail mutton products in beijing china |
| topic | sheep Sarcocystis spp. N. caninum T. gondii prevalence |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2022.1018788/full |
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