Electrical charge on ferroelectric nanocomposite membranes enhances SHED neural differentiation
Stem cells from human exfoliated deciduous teeth (SHED) uniquely exhibit high proliferative and neurogenic potential. Charged biomaterials have been demonstrated to promote neural differentiation of stem cells, but the dose-response effect of electrical stimuli from these materials on neural differe...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2023-02-01
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| Series: | Bioactive Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X22002183 |
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| author | Xiaochan Li Boon Chin Heng Yunyang Bai Qianqian Wang Min Gao Ying He Xinwen Zhang Xuliang Deng Xuehui Zhang |
| author_facet | Xiaochan Li Boon Chin Heng Yunyang Bai Qianqian Wang Min Gao Ying He Xinwen Zhang Xuliang Deng Xuehui Zhang |
| author_sort | Xiaochan Li |
| collection | DOAJ |
| description | Stem cells from human exfoliated deciduous teeth (SHED) uniquely exhibit high proliferative and neurogenic potential. Charged biomaterials have been demonstrated to promote neural differentiation of stem cells, but the dose-response effect of electrical stimuli from these materials on neural differentiation of SHED remains to be elucidated. Here, by utilizing different annealing temperatures prior to corona poling treatment, BaTiO3/P(VDF-TrFE) ferroelectric nanocomposite membranes with varying charge polarization intensity (d33 ≈ 0, 4, 12 and 19 pC N−1) were fabricated. Enhanced expression of neural markers, increased cell elongation and more prominent neurite outgrowths were observed with increasing surface charge of the nanocomposite membrane indicating a dose-response effect of surface electrical charge on SHED neural differentiation. Further investigations of the underlying molecular mechanisms revealed that intracellular calcium influx, focal adhesion formation, FAK-ERK mechanosensing pathway and neurogenic-related ErbB signaling pathway were implicated in the enhancement of SHED neural differentiation by surface electrical charge. Hence, this study confirms the dose-response effect of biomaterial surface charge on SHED neural differentiation and provides preliminary insights into the molecular mechanisms and signaling pathways involved. |
| first_indexed | 2024-04-12T00:00:30Z |
| format | Article |
| id | doaj.art-4e7bbdfa7d474dee92da0992724d3008 |
| institution | Directory Open Access Journal |
| issn | 2452-199X |
| language | English |
| last_indexed | 2024-04-24T08:40:57Z |
| publishDate | 2023-02-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Bioactive Materials |
| spelling | doaj.art-4e7bbdfa7d474dee92da0992724d30082024-04-16T15:59:09ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2023-02-01208192Electrical charge on ferroelectric nanocomposite membranes enhances SHED neural differentiationXiaochan Li0Boon Chin Heng1Yunyang Bai2Qianqian Wang3Min Gao4Ying He5Xinwen Zhang6Xuliang Deng7Xuehui Zhang8Department of Geriatric Dentistry, Peking University School and Hospital of Stomatology, Beijing, 100081, PR China; Department of Dental Materials & Dental Medical Devices Testing Center, Peking University School and Hospital of Stomatology, Beijing, 100081, PR China; Department of Prosthodontics, Peking University School and Hospital of Stomatology, Beijing, 100081, PR ChinaCentral Laboratory, Peking University School and Hospital of Stomatology, Beijing, 100081, PR ChinaDepartment of Geriatric Dentistry, Peking University School and Hospital of Stomatology, Beijing, 100081, PR ChinaDepartment of Geriatric Dentistry, Peking University School and Hospital of Stomatology, Beijing, 100081, PR ChinaDepartment of Geriatric Dentistry, Peking University School and Hospital of Stomatology, Beijing, 100081, PR ChinaDepartment of Geriatric Dentistry, Peking University School and Hospital of Stomatology, Beijing, 100081, PR ChinaCenter of Implant Dentistry, School and Hospital of Stomatology, China Medical University, Liaoning Provincial Key Laboratory of Oral Diseases, Shenyang, 110002, PR China; Corresponding author.Department of Geriatric Dentistry, Peking University School and Hospital of Stomatology, Beijing, 100081, PR China; National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, NMPA Key Laboratory for Dental Materials, Beijing Laboratory of Biomedical Materials, Peking University School and Hospital of Stomatology, Beijing, 100081, PR China; Corresponding author. Department of Geriatric Dentistry, Peking University School and Hospital of Stomatology, Beijing, 100081, PR China.Department of Dental Materials & Dental Medical Devices Testing Center, Peking University School and Hospital of Stomatology, Beijing, 100081, PR China; National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, NMPA Key Laboratory for Dental Materials, Beijing Laboratory of Biomedical Materials, Peking University School and Hospital of Stomatology, Beijing, 100081, PR China; Corresponding author. Department of Dental Materials & Dental Medical Devices Testing Center, Peking University School and Hospital of Stomatology, Beijing, 100081, PR China.Stem cells from human exfoliated deciduous teeth (SHED) uniquely exhibit high proliferative and neurogenic potential. Charged biomaterials have been demonstrated to promote neural differentiation of stem cells, but the dose-response effect of electrical stimuli from these materials on neural differentiation of SHED remains to be elucidated. Here, by utilizing different annealing temperatures prior to corona poling treatment, BaTiO3/P(VDF-TrFE) ferroelectric nanocomposite membranes with varying charge polarization intensity (d33 ≈ 0, 4, 12 and 19 pC N−1) were fabricated. Enhanced expression of neural markers, increased cell elongation and more prominent neurite outgrowths were observed with increasing surface charge of the nanocomposite membrane indicating a dose-response effect of surface electrical charge on SHED neural differentiation. Further investigations of the underlying molecular mechanisms revealed that intracellular calcium influx, focal adhesion formation, FAK-ERK mechanosensing pathway and neurogenic-related ErbB signaling pathway were implicated in the enhancement of SHED neural differentiation by surface electrical charge. Hence, this study confirms the dose-response effect of biomaterial surface charge on SHED neural differentiation and provides preliminary insights into the molecular mechanisms and signaling pathways involved.http://www.sciencedirect.com/science/article/pii/S2452199X22002183Surface chargeElectric polarizationDose-response effectElectrical microenvironmentNeurogenesis |
| spellingShingle | Xiaochan Li Boon Chin Heng Yunyang Bai Qianqian Wang Min Gao Ying He Xinwen Zhang Xuliang Deng Xuehui Zhang Electrical charge on ferroelectric nanocomposite membranes enhances SHED neural differentiation Bioactive Materials Surface charge Electric polarization Dose-response effect Electrical microenvironment Neurogenesis |
| title | Electrical charge on ferroelectric nanocomposite membranes enhances SHED neural differentiation |
| title_full | Electrical charge on ferroelectric nanocomposite membranes enhances SHED neural differentiation |
| title_fullStr | Electrical charge on ferroelectric nanocomposite membranes enhances SHED neural differentiation |
| title_full_unstemmed | Electrical charge on ferroelectric nanocomposite membranes enhances SHED neural differentiation |
| title_short | Electrical charge on ferroelectric nanocomposite membranes enhances SHED neural differentiation |
| title_sort | electrical charge on ferroelectric nanocomposite membranes enhances shed neural differentiation |
| topic | Surface charge Electric polarization Dose-response effect Electrical microenvironment Neurogenesis |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X22002183 |
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