From intestine to beyond: Salmonella entry factors display distinct transcription pattern upon infection in murine models
The infectious process of bacteria of the genus Salmonella requires the finely regulated use of various virulence factors. Among them, the type 3 secretion system-1 (T3SS-1) and the Rck and PagN invasins are involved in the internalization of the pathogen within eukaryotic cells, but their precise r...
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| Format: | Article |
| Language: | English |
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The Royal Society
2024-01-01
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| Series: | Open Biology |
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| Online Access: | https://royalsocietypublishing.org/doi/10.1098/rsob.230312 |
| _version_ | 1797353662288232448 |
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| author | Michaël Koczerka Isabelle Lantier Marie Morillon Justine Deperne Camille D. Clamagirand Isabelle Virlogeux-Payant Olivier Grépinet |
| author_facet | Michaël Koczerka Isabelle Lantier Marie Morillon Justine Deperne Camille D. Clamagirand Isabelle Virlogeux-Payant Olivier Grépinet |
| author_sort | Michaël Koczerka |
| collection | DOAJ |
| description | The infectious process of bacteria of the genus Salmonella requires the finely regulated use of various virulence factors. Among them, the type 3 secretion system-1 (T3SS-1) and the Rck and PagN invasins are involved in the internalization of the pathogen within eukaryotic cells, but their precise role in the host and in the pathogenic process is still poorly understood. In this study, we aimed to determine the kinetics of expression of these entry factors in a typhoid fever-like and a gastroenteritis model in mice by in vivo imaging using bioluminescent Salmonella Typhimurium reporter strains carrying chromosomal transcriptional fusions. Only pagN and T3SS-1 transcription has been clearly identified. Independently of the pathological model, the caecum was identified as the main transcription site of both pagN and the T3SS-1-encoding gene both at early and late stages of the infection. An intense transcription of pagN was also observed in deep organs in the typhoid fever-like model, while that of T3SS-1 remained quite sporadic in these organs, and mainly focused on the intestine all along the infection. This work will help to understand the respective role of these entry factors at the cellular level in the pathogenesis of Salmonella in vivo. |
| first_indexed | 2024-03-08T13:34:15Z |
| format | Article |
| id | doaj.art-4e7e6278292b4e18983bb1f65948559e |
| institution | Directory Open Access Journal |
| issn | 2046-2441 |
| language | English |
| last_indexed | 2024-03-08T13:34:15Z |
| publishDate | 2024-01-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Open Biology |
| spelling | doaj.art-4e7e6278292b4e18983bb1f65948559e2024-01-17T00:05:15ZengThe Royal SocietyOpen Biology2046-24412024-01-0114110.1098/rsob.230312From intestine to beyond: Salmonella entry factors display distinct transcription pattern upon infection in murine modelsMichaël Koczerka0Isabelle Lantier1Marie Morillon2Justine Deperne3Camille D. Clamagirand4Isabelle Virlogeux-Payant5Olivier Grépinet6INRAE, Université de Tours, ISP, 37380, Nouzilly, FranceINRAE, Université de Tours, ISP, 37380, Nouzilly, FranceINRAE, Université de Tours, ISP, 37380, Nouzilly, FranceINRAE, Université de Tours, ISP, 37380, Nouzilly, FranceINRAE, Université de Tours, ISP, 37380, Nouzilly, FranceINRAE, Université de Tours, ISP, 37380, Nouzilly, FranceINRAE, Université de Tours, ISP, 37380, Nouzilly, FranceThe infectious process of bacteria of the genus Salmonella requires the finely regulated use of various virulence factors. Among them, the type 3 secretion system-1 (T3SS-1) and the Rck and PagN invasins are involved in the internalization of the pathogen within eukaryotic cells, but their precise role in the host and in the pathogenic process is still poorly understood. In this study, we aimed to determine the kinetics of expression of these entry factors in a typhoid fever-like and a gastroenteritis model in mice by in vivo imaging using bioluminescent Salmonella Typhimurium reporter strains carrying chromosomal transcriptional fusions. Only pagN and T3SS-1 transcription has been clearly identified. Independently of the pathological model, the caecum was identified as the main transcription site of both pagN and the T3SS-1-encoding gene both at early and late stages of the infection. An intense transcription of pagN was also observed in deep organs in the typhoid fever-like model, while that of T3SS-1 remained quite sporadic in these organs, and mainly focused on the intestine all along the infection. This work will help to understand the respective role of these entry factors at the cellular level in the pathogenesis of Salmonella in vivo.https://royalsocietypublishing.org/doi/10.1098/rsob.230312SalmonellainvasionT3SS-1invasinsmurine modelsin vivo imaging |
| spellingShingle | Michaël Koczerka Isabelle Lantier Marie Morillon Justine Deperne Camille D. Clamagirand Isabelle Virlogeux-Payant Olivier Grépinet From intestine to beyond: Salmonella entry factors display distinct transcription pattern upon infection in murine models Open Biology Salmonella invasion T3SS-1 invasins murine models in vivo imaging |
| title | From intestine to beyond: Salmonella entry factors display distinct transcription pattern upon infection in murine models |
| title_full | From intestine to beyond: Salmonella entry factors display distinct transcription pattern upon infection in murine models |
| title_fullStr | From intestine to beyond: Salmonella entry factors display distinct transcription pattern upon infection in murine models |
| title_full_unstemmed | From intestine to beyond: Salmonella entry factors display distinct transcription pattern upon infection in murine models |
| title_short | From intestine to beyond: Salmonella entry factors display distinct transcription pattern upon infection in murine models |
| title_sort | from intestine to beyond salmonella entry factors display distinct transcription pattern upon infection in murine models |
| topic | Salmonella invasion T3SS-1 invasins murine models in vivo imaging |
| url | https://royalsocietypublishing.org/doi/10.1098/rsob.230312 |
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