In Vitro Evaluation of Lavandula angustifolia Essential Oil on Anti-Toxoplasma Activity
The current methods of treating toxoplasmosis have a number of side effects, and these therapies are only effective against the acute stage of the disease. Thus, development of new low toxicity and efficient anti-Toxoplasma drugs is extremely important. Natural products are important sources for scr...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-09-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2021.755715/full |
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| author | Na Yao Na Yao Na Yao Jia-Kang He Ming Pan Ming Pan Ming Pan Zhao-Feng Hou Zhao-Feng Hou Zhao-Feng Hou Jin-Jun Xu Jin-Jun Xu Jin-Jun Xu Yi Yang Jian-Ping Tao Jian-Ping Tao Jian-Ping Tao Si-Yang Huang Si-Yang Huang |
| author_facet | Na Yao Na Yao Na Yao Jia-Kang He Ming Pan Ming Pan Ming Pan Zhao-Feng Hou Zhao-Feng Hou Zhao-Feng Hou Jin-Jun Xu Jin-Jun Xu Jin-Jun Xu Yi Yang Jian-Ping Tao Jian-Ping Tao Jian-Ping Tao Si-Yang Huang Si-Yang Huang |
| author_sort | Na Yao |
| collection | DOAJ |
| description | The current methods of treating toxoplasmosis have a number of side effects, and these therapies are only effective against the acute stage of the disease. Thus, development of new low toxicity and efficient anti-Toxoplasma drugs is extremely important. Natural products are important sources for screening new drugs; among them, essential oils (EOs) have efficacy in anti-bacterial, anti-inflammatory, anti-insect, and other aspects. In this study, 16 EOs were screened for their anti-T. gondii activity. Lavandula angustifolia essential oil (La EO)was found to have an anti-parasitic effect on T. gondii. The cytotoxicity of La EO was firstly evaluated using the MTT assay on human foreskin fibroblast (HFF) cells, and then the anti-T. gondii activity was evaluated by plaque assay. Finally, the invasion experiment and electron microscope observation were used to study the mechanism of La EO in anti-toxoplasma activity. The results indicated that the CC50 of La EO was 4.48 mg/ml and that La EO had activity against T. gondii and the inhibition was in a dose-dependent manner under safe concentrations. La EO was able to reduce T. gondii invasion, which may be due to its detrimental effect on changes of the morphology of tachyzoites. These findings indicated that La EO could be a potential drug for treating toxoplasmosis. |
| first_indexed | 2024-12-24T03:53:49Z |
| format | Article |
| id | doaj.art-4e819c0106dc4119b2b683184aca95c9 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-24T03:53:49Z |
| publishDate | 2021-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-4e819c0106dc4119b2b683184aca95c92022-12-21T17:16:30ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882021-09-011110.3389/fcimb.2021.755715755715In Vitro Evaluation of Lavandula angustifolia Essential Oil on Anti-Toxoplasma ActivityNa Yao0Na Yao1Na Yao2Jia-Kang He3Ming Pan4Ming Pan5Ming Pan6Zhao-Feng Hou7Zhao-Feng Hou8Zhao-Feng Hou9Jin-Jun Xu10Jin-Jun Xu11Jin-Jun Xu12Yi Yang13Jian-Ping Tao14Jian-Ping Tao15Jian-Ping Tao16Si-Yang Huang17Si-Yang Huang18Institute of Comparative Medicine, College of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaJiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis, Yangzhou University, Yangzhou, ChinaJiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning, ChinaInstitute of Comparative Medicine, College of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaJiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis, Yangzhou University, Yangzhou, ChinaJiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, ChinaInstitute of Comparative Medicine, College of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaJiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis, Yangzhou University, Yangzhou, ChinaJiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, ChinaInstitute of Comparative Medicine, College of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaJiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis, Yangzhou University, Yangzhou, ChinaJiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, ChinaCollege of Animal Sciences, Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, ChinaInstitute of Comparative Medicine, College of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaJiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis, Yangzhou University, Yangzhou, ChinaJiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, ChinaInstitute of Comparative Medicine, College of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, ChinaThe current methods of treating toxoplasmosis have a number of side effects, and these therapies are only effective against the acute stage of the disease. Thus, development of new low toxicity and efficient anti-Toxoplasma drugs is extremely important. Natural products are important sources for screening new drugs; among them, essential oils (EOs) have efficacy in anti-bacterial, anti-inflammatory, anti-insect, and other aspects. In this study, 16 EOs were screened for their anti-T. gondii activity. Lavandula angustifolia essential oil (La EO)was found to have an anti-parasitic effect on T. gondii. The cytotoxicity of La EO was firstly evaluated using the MTT assay on human foreskin fibroblast (HFF) cells, and then the anti-T. gondii activity was evaluated by plaque assay. Finally, the invasion experiment and electron microscope observation were used to study the mechanism of La EO in anti-toxoplasma activity. The results indicated that the CC50 of La EO was 4.48 mg/ml and that La EO had activity against T. gondii and the inhibition was in a dose-dependent manner under safe concentrations. La EO was able to reduce T. gondii invasion, which may be due to its detrimental effect on changes of the morphology of tachyzoites. These findings indicated that La EO could be a potential drug for treating toxoplasmosis.https://www.frontiersin.org/articles/10.3389/fcimb.2021.755715/fullToxoplasma gondiinatural medicineLavandula angustifolia essential oilin vitrotreatment |
| spellingShingle | Na Yao Na Yao Na Yao Jia-Kang He Ming Pan Ming Pan Ming Pan Zhao-Feng Hou Zhao-Feng Hou Zhao-Feng Hou Jin-Jun Xu Jin-Jun Xu Jin-Jun Xu Yi Yang Jian-Ping Tao Jian-Ping Tao Jian-Ping Tao Si-Yang Huang Si-Yang Huang In Vitro Evaluation of Lavandula angustifolia Essential Oil on Anti-Toxoplasma Activity Frontiers in Cellular and Infection Microbiology Toxoplasma gondii natural medicine Lavandula angustifolia essential oil in vitro treatment |
| title | In Vitro Evaluation of Lavandula angustifolia Essential Oil on Anti-Toxoplasma Activity |
| title_full | In Vitro Evaluation of Lavandula angustifolia Essential Oil on Anti-Toxoplasma Activity |
| title_fullStr | In Vitro Evaluation of Lavandula angustifolia Essential Oil on Anti-Toxoplasma Activity |
| title_full_unstemmed | In Vitro Evaluation of Lavandula angustifolia Essential Oil on Anti-Toxoplasma Activity |
| title_short | In Vitro Evaluation of Lavandula angustifolia Essential Oil on Anti-Toxoplasma Activity |
| title_sort | in vitro evaluation of lavandula angustifolia essential oil on anti toxoplasma activity |
| topic | Toxoplasma gondii natural medicine Lavandula angustifolia essential oil in vitro treatment |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2021.755715/full |
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